PDB entry 7am7

View 7am7 on RCSB PDB site
Description: crystal structure of peptiligase mutant - m222p/l217h/a225n/f189w/n218d
Deposited on 2020-10-08, released 2021-02-17
The last revision was dated 2021-03-24, with a file datestamp of 2021-03-19.
Experiment type: XRAY
Resolution: 2.61 Å
R-factor: N/A
AEROSPACI score: 0.26 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: subtilisin bpn'
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00782 (0-265)
      • engineered mutation (1-2)
      • engineered mutation (4)
      • engineered mutation (42)
      • engineered mutation (49)
      • engineered mutation (73)
      • engineered mutation (146)
      • engineered mutation (156)
      • engineered mutation (159)
      • engineered mutation (178-179)
      • engineered mutation (196)
      • engineered mutation (207-208)
      • engineered mutation (211-212)
      • engineered mutation (215)
      • engineered mutation (244)
      • engineered mutation (261)
      • expression tag (266)
  • Chain 'B':
    Compound: subtilisin bpn'
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00782 (0-265)
      • engineered mutation (1-2)
      • engineered mutation (4)
      • engineered mutation (42)
      • engineered mutation (49)
      • engineered mutation (73)
      • engineered mutation (146)
      • engineered mutation (156)
      • engineered mutation (159)
      • engineered mutation (178-179)
      • engineered mutation (196)
      • engineered mutation (207-208)
      • engineered mutation (211-212)
      • engineered mutation (215)
      • engineered mutation (244)
      • engineered mutation (261)
      • expression tag (266-267)
  • Chain 'C':
    Compound: subtilisin bpn'
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00782 (0-265)
      • engineered mutation (1-2)
      • engineered mutation (4)
      • engineered mutation (42)
      • engineered mutation (49)
      • engineered mutation (73)
      • engineered mutation (146)
      • engineered mutation (156)
      • engineered mutation (159)
      • engineered mutation (178-179)
      • engineered mutation (196)
      • engineered mutation (207-208)
      • engineered mutation (211-212)
      • engineered mutation (215)
      • engineered mutation (244)
      • engineered mutation (261)
  • Chain 'P':
    Compound: Eglin C fragment
    Species: Hirudo medicinalis, synthetic [TaxId:6421]
    Database cross-references and differences (RAF-indexed):
    • PDB 7AM7
  • Heterogens: GOL, SO4, PGE, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >7am7A (A:)
akcvsygvsqikapalhsqgytgsnvkvavidsgidsshpdlnvaggasfvpsetnpfqd
nnshgthvagtvlavapsaslyavkvlgadgsgqyswiingiewaiannmdvinmslggp
sgsaalkaavdkavasgvvvvaaagnsgtsgssstvsypakypsviavgavdssnqrapw
ssvgpeldvmapgvsicstlpgnkygahdgtcpasnhvagaaalilskhpnwtntqvrss
lentatklgdsfyygkglinveaaaqhhhhhh

    Sequence, based on observed residues (ATOM records):
    >7am7A (A:)
akcvsygvsqikapalhsqgytgsnvkvavidsgidsshpdlnvaggasfvpsetnpfqd
nnshgthvagtvlavapsaslyavkvlgadgsgqyswiingiewaiannmdvinmslggp
sgsaalkaavdkavasgvvvvaaagnsgtsgssstvsypakypsviavgavdssnqrapw
ssvgpeldvmapgvsicstlpgnkygahdgtcpasnhvagaaalilskhpnwtntqvrss
lentatklgdsfyygkglinveaaaqh

  • Chain 'B':
    Sequence, based on SEQRES records:
    >7am7B (B:)
akcvsygvsqikapalhsqgytgsnvkvavidsgidsshpdlnvaggasfvpsetnpfqd
nnshgthvagtvlavapsaslyavkvlgadgsgqyswiingiewaiannmdvinmslggp
sgsaalkaavdkavasgvvvvaaagnsgtsgssstvsypakypsviavgavdssnqrapw
ssvgpeldvmapgvsicstlpgnkygahdgtcpasnhvagaaalilskhpnwtntqvrss
lentatklgdsfyygkglinveaaaqhhhhhh

    Sequence, based on observed residues (ATOM records):
    >7am7B (B:)
akcvsygvsqikapalhsqgytgsnvkvavidsgidsshpdlnvaggasfvpsetnpfqd
nnshgthvagtvlavapsaslyavkvlgadgsgqyswiingiewaiannmdvinmslggp
sgsaalkaavdkavasgvvvvaaagnsgtsgssstvsypakypsviavgavdssnqrapw
ssvgpeldvmapgvsicstlpgnkygahdgtcpasnhvagaaalilskhpnwtntqvrss
lentatklgdsfyygkglinveaaaqhh

  • Chain 'C':
    Sequence, based on SEQRES records:
    >7am7C (C:)
akcvsygvsqikapalhsqgytgsnvkvavidsgidsshpdlnvaggasfvpsetnpfqd
nnshgthvagtvlavapsaslyavkvlgadgsgqyswiingiewaiannmdvinmslggp
sgsaalkaavdkavasgvvvvaaagnsgtsgssstvsypakypsviavgavdssnqrapw
ssvgpeldvmapgvsicstlpgnkygahdgtcpasnhvagaaalilskhpnwtntqvrss
lentatklgdsfyygkglinveaaaqhhhhhh

    Sequence, based on observed residues (ATOM records):
    >7am7C (C:)
akcvsygvsqikapalhsqgytgsnvkvavidsgidsshpdlnvaggasfvpsetnpfqd
nnshgthvagtvlavapsaslyavkvlgadgsgqyswiingiewaiannmdvinmslggp
sgsaalkaavdkavasgvvvvaaagnsgtsgssstvsypakypsviavgavdssnqrapw
ssvgpeldvmapgvsicstlpgnkyahdgtcpasnhvagaaalilskhpnwtntqvrssl
entatklgdsfyygkglinveaaaq

  • Chain 'P':
    Sequence, based on SEQRES records:
    >7am7P (P:)
lpegspvtldlry

    Sequence, based on observed residues (ATOM records):
    >7am7P (P:)
spvtld