PDB entry 7am3

View 7am3 on RCSB PDB site
Description: Crystal structure of Peptiligase mutant - M222P
Class: ligase
Keywords: subtilisin, peptide ligase, LIGASE
Deposited on 2020-10-08, released 2021-02-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-03-24, with a file datestamp of 2021-03-19.
Experiment type: XRAY
Resolution: 1.61 Å
R-factor: N/A
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: subtilisin bpn'
    Species: Bacillus amyloliquefaciens [TaxId:1390]
    Gene: APR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00782 (0-265)
      • engineered mutation (1-2)
      • engineered mutation (4)
      • conflict (42)
      • engineered mutation (49)
      • conflict (73)
      • engineered mutation (146)
      • engineered mutation (156)
      • engineered mutation (159)
      • engineered mutation (178)
      • engineered mutation (196)
      • conflict (207)
      • engineered mutation (208)
      • engineered mutation (211-212)
      • engineered mutation (215)
      • engineered mutation (244)
      • engineered mutation (261)
      • expression tag (266-268)
    Domains in SCOPe 2.08: d7am3a1, d7am3a2
  • Heterogens: GOL, SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >7am3A (A:)
akcvsygvsqikapalhsqgytgsnvkvavidsgidsshpdlnvaggasfvpsetnpfqd
nnshgthvagtvlavapsaslyavkvlgadgsgqyswiingiewaiannmdvinmslggp
sgsaalkaavdkavasgvvvvaaagnsgtsgssstvsypakypsviavgavdssnqrapf
ssvgpeldvmapgvsicstlpgnkygalsgtcpasahvagaaalilskhpnwtntqvrss
lentatklgdsfyygkglinveaaaqhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >7am3A (A:)
akcvsygvsqikapalhsqgytgsnvkvavidsgidsshpdlnvaggasfvpsetnpfqd
nnshgthvagtvlavapsaslyavkvlgadgsgqyswiingiewaiannmdvinmslggp
sgsaalkaavdkavasgvvvvaaagnsgtsgssstvsypakypsviavgavdssnqrapf
ssvgpeldvmapgvsicstlpgnkygalsgtcpasahvagaaalilskhpnwtntqvrss
lentatklgdsfyygkglinveaaaqhhh