PDB entry 7a8h

View 7a8h on RCSB PDB site
Description: rsGreen0.7-K206A-H148S in the green-on state
Class: fluorescent protein
Keywords: Reversible photoswitchable fluorescent protein, FLUORESCENT PROTEIN
Deposited on 2020-08-30, released 2021-02-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-05-05, with a file datestamp of 2021-04-30.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: N/A
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059PIQ0 (36-End)
      • expression tag (35)
      • conflict (63)
      • chromophore (99)
      • conflict (102)
      • conflict (138)
      • engineered mutation (181)
      • conflict (183)
      • conflict (196)
      • conflict (238)
    Domains in SCOPe 2.08: d7a8ha1, d7a8ha2
  • Chain 'B':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059PIQ0 (36-End)
      • expression tag (35)
      • conflict (63)
      • chromophore (99)
      • conflict (102)
      • conflict (138)
      • engineered mutation (181)
      • conflict (183)
      • conflict (196)
      • conflict (238)
    Domains in SCOPe 2.08: d7a8hb1, d7a8hb2
  • Chain 'C':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059PIQ0 (36-End)
      • expression tag (34-35)
      • conflict (63)
      • chromophore (99)
      • conflict (102)
      • conflict (138)
      • engineered mutation (181)
      • conflict (183)
      • conflict (196)
      • conflict (238)
      • conflict (264)
    Domains in SCOPe 2.08: d7a8hc1, d7a8hc2
  • Chain 'D':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059PIQ0 (36-End)
      • expression tag (34-35)
      • conflict (63)
      • chromophore (99)
      • conflict (102)
      • conflict (138)
      • engineered mutation (181)
      • conflict (183)
      • conflict (196)
      • conflict (238)
      • conflict (264)
    Domains in SCOPe 2.08: d7a8hd1, d7a8hd2
  • Heterogens: EDO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >7a8hA (A:)
    mrgshhhhhhgmasmtggqqmgrdlyddddkdpmvskgeelftgvvpilveldgdvnghk
    fsvrgegegdatngkltlkficttgklpvpwptlvttlgygvlcfarypdhmkqhdffks
    ampegyvqertisfkddgyyktraevkfegdtlvnrielkgidfkedgnilghkleynfn
    ssnayitadkqkngiksnfkirhnvedgsvqladhyqqntpigdgpvllpdnhylstqna
    lskdpnekrdhmvllefvtaagitlgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >7a8hA (A:)
    skgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptlv
    ttlgygvlcfarypdhmkqhdffksampegyvqertisfkddgyyktraevkfegdtlvn
    rielkgidfkedgnilghkleynfnssnayitadkqkngiksnfkirhnvedgsvqladh
    yqqntpigdgpvllpdnhylstqnalskdpnekrdhmvllefvtaag
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >7a8hB (B:)
    mrgshhhhhhgmasmtggqqmgrdlyddddkdpmvskgeelftgvvpilveldgdvnghk
    fsvrgegegdatngkltlkficttgklpvpwptlvttlgygvlcfarypdhmkqhdffks
    ampegyvqertisfkddgyyktraevkfegdtlvnrielkgidfkedgnilghkleynfn
    ssnayitadkqkngiksnfkirhnvedgsvqladhyqqntpigdgpvllpdnhylstqna
    lskdpnekrdhmvllefvtaagitlgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >7a8hB (B:)
    skgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptlv
    ttlgygvlcfarypdhmkqhdffksampegyvqertisfkddgyyktraevkfegdtlvn
    rielkgidfkedgnilghkleynfnssnayitadkqkngiksnfkirhnvedgsvqladh
    yqqntpigdgpvllpdnhylstqnalskdpnekrdhmvllefvtaagit
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >7a8hC (C:)
    mrgshhhhhhgmasmtggqqmgrdlyddddkdpmvskgeelftgvvpilveldgdvnghk
    fsvrgegegdatngkltlkficttgklpvpwptlvttlgygvlcfarypdhmkqhdffks
    ampegyvqertisfkddgyyktraevkfegdtlvnrielkgidfkedgnilghkleynfn
    ssnayitadkqkngiksnfkirhnvedgsvqladhyqqntpigdgpvllpdnhylstqna
    lskdpnekrdhmvllefvtaagitlgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >7a8hC (C:)
    vskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptl
    vttlgygvlcfarypdhmkqhdffksampegyvqertisfkddgyyktraevkfegdtlv
    nrielkgidfkedgnilghkleynfnssnayitadkqkngiksnfkirhnvedgsvqlad
    hyqqntpigdgpvllpdnhylstqnalskdpnekrdhmvllefvtaagitlg
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >7a8hD (D:)
    mrgshhhhhhgmasmtggqqmgrdlyddddkdpmvskgeelftgvvpilveldgdvnghk
    fsvrgegegdatngkltlkficttgklpvpwptlvttlgygvlcfarypdhmkqhdffks
    ampegyvqertisfkddgyyktraevkfegdtlvnrielkgidfkedgnilghkleynfn
    ssnayitadkqkngiksnfkirhnvedgsvqladhyqqntpigdgpvllpdnhylstqna
    lskdpnekrdhmvllefvtaagitlgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >7a8hD (D:)
    vskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptl
    vttlgygvlcfarypdhmkqhdffksampegyvqertisfkddgyyktraevkfegdtlv
    nrielkgidfkedgnilghkleynfnssnayitadkqkngiksnfkirhnvedgsvqlad
    hyqqntpigdgpvllpdnhylstqnalskdpnekrdhmvllefvtaagitl