PDB entry 6xox

View 6xox on RCSB PDB site
Description: cryo-EM of human GLP-1R bound to non-peptide agonist LY3502970
Class: membrane protein
Keywords: Class B GPCR, glucagon-like peptide-1 receptor, G protein nucleotide exchange factor., MEMBRANE PROTEIN
Deposited on 2020-07-07, released 2020-11-18
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-12-09, with a file datestamp of 2020-12-04.
Experiment type: EM
Resolution: 3.1 Å
R-factor: N/A
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Alpha subunit of Gs with N-terminus swapped with equivalent residues in Gi,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q5FWY2 (18-End)
      • expression tag (4-17)
    • Uniprot Q5JWF2 (Start-372)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6xoxb_
  • Chain 'E':
    Compound: single-chain variable fragment scFv16
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6XOX
    Domains in SCOPe 2.08: d6xoxe1, d6xoxe2
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6xoxg_
  • Chain 'N':
    Compound: nanobody 35
    Species: Lama glama [TaxId:9844]
    Database cross-references and differences (RAF-indexed):
    • PDB 6XOX
    Domains in SCOPe 2.08: d6xoxn1, d6xoxn2
  • Chain 'R':
    Compound: Glucagon-like peptide 1 receptor
    Species: Homo sapiens [TaxId:9606]
    Gene: GLP1R
    Database cross-references and differences (RAF-indexed):
    • Uniprot P43220
      • variant (282)
  • Heterogens: V6G

PDB Chain Sequences:

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6xoxB (B:)
mhhhhhhgssgseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrt
lrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgn
yvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwd
ietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdin
aicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyd
dfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records): (download) 
    >6xoxB (B:)
seldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyam
hwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnic
siynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttft
ghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnaf
atgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalk
adragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >6xoxE (E:)
dvqlvesggglvqpggsrklscsasgfafssfgmhwvrqapekglewvayissgsgtiyy
adtvkgrftisrddpkntlflqmtslrsedtamyycvrsiyyygsspfdfwgqgttltvs
sggggsggggsggggsdivmtqatssvpvtpgesvsiscrssksllhsngntylywflqr
pgqspqlliyrmsnlasgvpdrfsgsgsgtaftltisrleaedvgvyycmqhleypltfg
agtklelkaaahhhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6xoxE (E:)
vqlvesggglvqpggsrklscsasgfafssfgmhwvrqapekglewvayissgsgtiyya
dtvkgrftisrddpkntlflqmtslrsedtamyycvrsiyyygsspfdfwgqgttltvss
sdivmtqatssvpvtpgesvsiscrssksllhsngntylywflqrpgqspqlliyrmsnl
asgvpdrfsgsgsgtaftltisrleaedvgvyycmqhleypltfgagtklel

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >6xoxG (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records): (download) 
    >6xoxG (G:)
ntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfre

  • Chain 'N':
    Sequence, based on SEQRES records: (download) 
    >6xoxN (N:)
mkyllptaaagllllaaqpamaqvqlqesggglvqpggslrlscaasgftfsnykmnwvr
qapgkglewvsdisqsgasisytgsvkgrftisrdnakntlylqmnslkpedtavyycar
cpapftrdcfdvtsttyayrgqgtqvtvsshhhhhhepea

    Sequence, based on observed residues (ATOM records): (download) 
    >6xoxN (N:)
qvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisy
tgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgq
gtqvtvss

  • Chain 'R':
    No sequence available.