PDB entry 6wz4

View 6wz4 on RCSB PDB site
Description: Complex of mutant (K173M) of Pseudomonas 7A Glutaminase-Asparaginase with L-Asp at pH 6. Covalent acyl-enzyme intermediate
Class: hydrolase
Keywords: HYDROLASE, Amidohydrolase, Glutaminase-Asparaginase, L-Asn/L-Gln-Hydrolase
Deposited on 2020-05-13, released 2020-10-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-10-21, with a file datestamp of 2020-10-16.
Experiment type: XRAY
Resolution: 1.58 Å
R-factor: N/A
AEROSPACI score: 0.53 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: glutaminase-asparaginase
    Species: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) [TaxId:160488]
    Gene: ansB, PP_2453
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q88K39 (Start-336)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6wz4a_
  • Chain 'B':
    Compound: glutaminase-asparaginase
    Species: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) [TaxId:160488]
    Gene: ansB, PP_2453
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q88K39 (Start-336)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6wz4b_
  • Chain 'C':
    Compound: glutaminase-asparaginase
    Species: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) [TaxId:160488]
    Gene: ansB, PP_2453
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q88K39 (Start-336)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6wz4c_
  • Chain 'D':
    Compound: glutaminase-asparaginase
    Species: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) [TaxId:160488]
    Gene: ansB, PP_2453
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q88K39 (Start-336)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6wz4d_
  • Heterogens: ASP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6wz4A (A:)
keaetqqklanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrge
qvmqiasesisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpi
vvvgsmrpgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikte
afksawgpmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtayka
laqngakalihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkn
dwvvahdlnpqkarilamvamtktqdskelqrifwey

    Sequence, based on observed residues (ATOM records): (download) 
    >6wz4A (A:)
klanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrgeqvmqias
esisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpivvvgsmr
pgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikteafksawg
pmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtaykalaqngak
alihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkndwvvahd
lnpqkarilamvamtktqdskelqrifwey

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6wz4B (B:)
keaetqqklanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrge
qvmqiasesisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpi
vvvgsmrpgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikte
afksawgpmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtayka
laqngakalihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkn
dwvvahdlnpqkarilamvamtktqdskelqrifwey

    Sequence, based on observed residues (ATOM records): (download) 
    >6wz4B (B:)
klanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrgeqvmqias
esisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpivvvgsmr
pgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikteafksawg
pmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtaykalaqngak
alihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkndwvvahd
lnpqkarilamvamtktqdskelqrifwey

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6wz4C (C:)
keaetqqklanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrge
qvmqiasesisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpi
vvvgsmrpgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikte
afksawgpmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtayka
laqngakalihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkn
dwvvahdlnpqkarilamvamtktqdskelqrifwey

    Sequence, based on observed residues (ATOM records): (download) 
    >6wz4C (C:)
klanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrgeqvmqias
esisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpivvvgsmr
pgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikteafksawg
pmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtaykalaqngak
alihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkndwvvahd
lnpqkarilamvamtktqdskelqrifwey

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6wz4D (D:)
keaetqqklanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrge
qvmqiasesisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpi
vvvgsmrpgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikte
afksawgpmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtayka
laqngakalihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkn
dwvvahdlnpqkarilamvamtktqdskelqrifwey

    Sequence, based on observed residues (ATOM records): (download) 
    >6wz4D (D:)
klanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrgeqvmqias
esisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpivvvgsmr
pgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikteafksawg
pmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtaykalaqngak
alihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkndwvvahd
lnpqkarilamvamtktqdskelqrifwey