PDB entry 6wyz

View 6wyz on RCSB PDB site
Description: Crystal structure of Pseudomonas 7A Glutaminase-Asparaginase (mutant K173M) in complex with D-Glu at pH 5.5
Class: hydrolase
Keywords: HYDROLASE, Amidohydrolase, Glutaminase-Asparaginase, L-Asn/L-Gln-Hydrolase
Deposited on 2020-05-13, released 2020-10-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-10-21, with a file datestamp of 2020-10-16.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: N/A
AEROSPACI score: 0.46 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: glutaminase-asparaginase
    Species: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) [TaxId:160488]
    Gene: ansB, PP_2453
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q88K39 (Start-336)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6wyza_
  • Chain 'B':
    Compound: glutaminase-asparaginase
    Species: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) [TaxId:160488]
    Gene: ansB, PP_2453
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q88K39 (Start-336)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6wyzb_
  • Chain 'C':
    Compound: glutaminase-asparaginase
    Species: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) [TaxId:160488]
    Gene: ansB, PP_2453
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q88K39 (Start-336)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6wyzc_
  • Chain 'D':
    Compound: glutaminase-asparaginase
    Species: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) [TaxId:160488]
    Gene: ansB, PP_2453
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q88K39 (Start-336)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6wyzd_
  • Heterogens: DGL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6wyzA (A:)
keaetqqklanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrge
qvmqiasesisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpi
vvvgsmrpgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikte
afksawgpmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtayka
laqngakalihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkn
dwvvahdlnpqkarilamvamtktqdskelqrifwey

    Sequence, based on observed residues (ATOM records): (download) 
    >6wyzA (A:)
lanvvilatggtiagvdkliagvpeladianvrgeqvmqiasesisnddllklgkrvael
aeskdvdgivithgtdtleetafflnlvektdkpivvvgsmrpgtamsadgmlnlynava
vasdkqsrgkgvlvtmndeiqsgrdvsmavnikteafksawgpmgmvvegksywfrlpak
rhtvnsefdikqisslpqvdiaygygnvtdtaykalaqngakalihagtgngsvssrvvp
alqelrkngvqiirsshvnqggfvlrnaeqpddkndwvvahdlnpqkarilamvamtktq
dskelqrifwey

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6wyzB (B:)
keaetqqklanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrge
qvmqiasesisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpi
vvvgsmrpgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikte
afksawgpmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtayka
laqngakalihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkn
dwvvahdlnpqkarilamvamtktqdskelqrifwey

    Sequence, based on observed residues (ATOM records): (download) 
    >6wyzB (B:)
klanvvilatggtiaglgvdkliagvpeladianvrgeqvmqiasesisnddllklgkrv
aelaeskdvdgivithgtdtleetafflnlvektdkpivvvgsmrpgtamsadgmlnlyn
avavasdkqsrgkgvlvtmndeiqsgrdvsmavnikteafksawgpmgmvvegksywfrl
pakrhtvnsefdikqisslpqvdiaygygnvtdtaykalaqngakalihagtgngsvssr
vvpalqelrkngvqiirsshvnqggfvlrnaeqpddkndwvvahdlnpqkarilamvamt
ktqdskelqrifwey

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6wyzC (C:)
keaetqqklanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrge
qvmqiasesisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpi
vvvgsmrpgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikte
afksawgpmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtayka
laqngakalihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkn
dwvvahdlnpqkarilamvamtktqdskelqrifwey

    Sequence, based on observed residues (ATOM records): (download) 
    >6wyzC (C:)
klanvvilatggtiaggvdkliagvpeladianvrgeqvmqiasesisnddllklgkrva
elaeskdvdgivithgtdtleetafflnlvektdkpivvvgsmrpgtamsadgmlnlyna
vavasdkqsrgkgvlvtmndeiqsgrdvsmavnikteafksawgpmgmvvegksywfrlp
akrhtvnsefdikqisslpqvdiaygygnvtdtaykalaqngakalihagtgngsvssrv
vpalqelrkngvqiirsshvnqggfvlrnaeqpddkndwvvahdlnpqkarilamvamtk
tqdskelqrifwey

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6wyzD (D:)
keaetqqklanvvilatggtiagagasaansatyqaaklgvdkliagvpeladianvrge
qvmqiasesisnddllklgkrvaelaeskdvdgivithgtdtleetafflnlvektdkpi
vvvgsmrpgtamsadgmlnlynavavasdkqsrgkgvlvtmndeiqsgrdvsmavnikte
afksawgpmgmvvegksywfrlpakrhtvnsefdikqisslpqvdiaygygnvtdtayka
laqngakalihagtgngsvssrvvpalqelrkngvqiirsshvnqggfvlrnaeqpddkn
dwvvahdlnpqkarilamvamtktqdskelqrifwey

    Sequence, based on observed residues (ATOM records): (download) 
    >6wyzD (D:)
lanvvilatggtiagvdkliagvpeladianvrgeqvmqiasesisnddllklgkrvael
aeskdvdgivithgtdtleetafflnlvektdkpivvvgsmrpgtamsadgmlnlynava
vasdkqsrgkgvlvtmndeiqsgrdvsmavnikteafksawgpmgmvvegksywfrlpak
rhtvnsefdikqisslpqvdiaygygnvtdtaykalaqngakalihagtgngsvssrvvp
alqelrkngvqiirsshvnqggfvlrnaeqpddkndwvvahdlnpqkarilamvamtktq
dskelqrifwey