PDB entry 6wrf

View 6wrf on RCSB PDB site
Description: ClpX-ClpP complex bound to GFP-ssrA, recognition complex
Class: chaperone
Keywords: Protein degradation, AAA+ protease complex, CHAPERONE
Deposited on 2020-04-29, released 2020-11-04
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-11-04, with a file datestamp of 2020-10-30.
Experiment type: EM
Resolution: 3.14 Å
R-factor: N/A
AEROSPACI score: 0.21 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: ATP-dependent Clp protease ATP-binding subunit clpX
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpX, lopC, b0438, JW0428
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6H1
      • conflict (132)
      • conflict (371)
    Domains in SCOPe 2.08: d6wrfa_
  • Chain 'B':
    Compound: ATP-dependent Clp protease ATP-binding subunit clpX
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpX, lopC, b0438, JW0428
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6H1
      • conflict (132)
      • conflict (371)
    Domains in SCOPe 2.08: d6wrfb_
  • Chain 'C':
    Compound: ATP-dependent Clp protease ATP-binding subunit clpX
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpX, lopC, b0438, JW0428
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6H1 (25-End)
      • conflict (132)
      • conflict (371)
    Domains in SCOPe 2.08: d6wrfc_
  • Chain 'D':
    Compound: ATP-dependent Clp protease ATP-binding subunit clpX
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpX, lopC, b0438, JW0428
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6H1 (25-End)
      • conflict (132)
      • conflict (371)
    Domains in SCOPe 2.08: d6wrfd_
  • Chain 'E':
    Compound: ATP-dependent Clp protease ATP-binding subunit clpX
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpX, lopC, b0438, JW0428
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6H1 (25-End)
      • conflict (132)
      • conflict (371)
    Domains in SCOPe 2.08: d6wrfe_
  • Chain 'F':
    Compound: ATP-dependent Clp protease ATP-binding subunit clpX
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpX, lopC, b0438, JW0428
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6H1
      • conflict (132)
      • conflict (371)
    Domains in SCOPe 2.08: d6wrff_
  • Chain 'H':
    Compound: ATP-dependent Clp protease proteolytic subunit
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpP, lopP, b0437, JW0427
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6wrfh_
  • Chain 'I':
    Compound: ATP-dependent Clp protease proteolytic subunit
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpP, lopP, b0437, JW0427
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6wrfi_
  • Chain 'J':
    Compound: ATP-dependent Clp protease proteolytic subunit
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpP, lopP, b0437, JW0427
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6wrfj_
  • Chain 'K':
    Compound: ATP-dependent Clp protease proteolytic subunit
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpP, lopP, b0437, JW0427
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6wrfk_
  • Chain 'L':
    Compound: ATP-dependent Clp protease proteolytic subunit
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpP, lopP, b0437, JW0427
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6wrfl_
  • Chain 'M':
    Compound: ATP-dependent Clp protease proteolytic subunit
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpP, lopP, b0437, JW0427
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A6G7 (0-191)
      • expression tag (192-196)
    Domains in SCOPe 2.08: d6wrfm1, d6wrfm2
  • Chain 'N':
    Compound: ATP-dependent Clp protease proteolytic subunit
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: clpP, lopP, b0437, JW0427
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d6wrfn_
  • Chain 'S':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212
      • expression tag (267-273)
  • Heterogens: AGS, MG, ADP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6wrfA (A:)
mgsshhhhhhdydipttenlyfqgssalptpheirnhlddyvigqeqakkvlavavynhy
krlrngdtsngvelgksnilligptgsgktllaetlarlldvpftmadattlteagyvge
dveniiqkllqksdydvqkaqrgivyideidkisrksdnpsitrdvsgegvqqallklie
gtvaavppqggrkhpqqeflqvdtskilficggafagldkvishrvetgsgigfgatvka
ksdkasegellaqvepedlikfglipefigrlpvvatlnelseealiqilkepknaltkq
yqalfnlegvdlefrdealdaiakkamarktgarglrsiveaalldtmydlpsmedvekv
videsvidgqseplliygkpeaqqasge

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfA (A:)
lptpheirnhlddyvigqeqakkvlavavynhykrlrngdtsngvelgksnilligptgs
gktllaetlarlldvpftmadattlteagyvgedveniiqkllqksdydvqkaqrgivyi
deidkisrksdnpsitrdvsgegvqqallkliegtvaavppqggrkhpqqeflqvdtski
lficggafagldkvishrvetgsgigfgatvkaksdkasegellaqvepedlikfglipe
figrlpvvatlnelseealiqilkepknaltkqyqalfnlegvdlefrdealdaiakkam
arktgarglrsiveaalldtmydlpsmedvekvvidesvidgqseplliyg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6wrfB (B:)
mgsshhhhhhdydipttenlyfqgssalptpheirnhlddyvigqeqakkvlavavynhy
krlrngdtsngvelgksnilligptgsgktllaetlarlldvpftmadattlteagyvge
dveniiqkllqksdydvqkaqrgivyideidkisrksdnpsitrdvsgegvqqallklie
gtvaavppqggrkhpqqeflqvdtskilficggafagldkvishrvetgsgigfgatvka
ksdkasegellaqvepedlikfglipefigrlpvvatlnelseealiqilkepknaltkq
yqalfnlegvdlefrdealdaiakkamarktgarglrsiveaalldtmydlpsmedvekv
videsvidgqseplliygkpeaqqasge

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfB (B:)
alptpheirnhlddyvigqeqakkvlavavynhykrlrngdtsngvelgksnilligptg
sgktllaetlarlldvpftmadattlteagyvgedveniiqkllqksdydvqkaqrgivy
ideidkisrksdnpsitrdvsgegvqqallkliegtvaavppqggrkhpqqeflqvdtsk
ilficggafagldkvishrvetgsgigfgatvkaksdkasegellaqvepedlikfglip
efigrlpvvatlnelseealiqilkepknaltkqyqalfnlegvdlefrdealdaiakka
marktgarglrsiveaalldtmydlpsmedvekvvidesvidgqseplliyg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6wrfC (C:)
mgsshhhhhhdydipttenlyfqgssalptpheirnhlddyvigqeqakkvlavavynhy
krlrngdtsngvelgksnilligptgsgktllaetlarlldvpftmadattlteagyvge
dveniiqkllqksdydvqkaqrgivyideidkisrksdnpsitrdvsgegvqqallklie
gtvaavppqggrkhpqqeflqvdtskilficggafagldkvishrvetgsgigfgatvka
ksdkasegellaqvepedlikfglipefigrlpvvatlnelseealiqilkepknaltkq
yqalfnlegvdlefrdealdaiakkamarktgarglrsiveaalldtmydlpsmedvekv
videsvidgqseplliygkpeaqqasge

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfC (C:)
salptpheirnhlddyvigqeqakkvlavavynhykrlrngdtsngvelgksnilligpt
gsgktllaetlarlldvpftmadattlteagyvgedveniiqkllqksdydvqkaqrgiv
yideidkisrksdnpsitrdvsgegvqqallkliegtvaavppqggrkhpqqeflqvdts
kilficggafagldkvishrvetgsgigfgatvkaksdkasegellaqvepedlikfgli
pefigrlpvvatlnelseealiqilkepknaltkqyqalfnlegvdlefrdealdaiakk
amarktgarglrsiveaalldtmydlpsmedvekvvidesvidgqseplliyg

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6wrfD (D:)
mgsshhhhhhdydipttenlyfqgssalptpheirnhlddyvigqeqakkvlavavynhy
krlrngdtsngvelgksnilligptgsgktllaetlarlldvpftmadattlteagyvge
dveniiqkllqksdydvqkaqrgivyideidkisrksdnpsitrdvsgegvqqallklie
gtvaavppqggrkhpqqeflqvdtskilficggafagldkvishrvetgsgigfgatvka
ksdkasegellaqvepedlikfglipefigrlpvvatlnelseealiqilkepknaltkq
yqalfnlegvdlefrdealdaiakkamarktgarglrsiveaalldtmydlpsmedvekv
videsvidgqseplliygkpeaqqasge

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfD (D:)
salptpheirnhlddyvigqeqakkvlavavynhykrlrngdtsngvelgksnilligpt
gsgktllaetlarlldvpftmadattlteagyvgedveniiqkllqksdydvqkaqrgiv
yideidkisrksdnpsitrdvsgegvqqallkliegtvaavppqggrkhpqqeflqvdts
kilficggafagldkvishrvetgsgigfgatvkaksdkasegellaqvepedlikfgli
pefigrlpvvatlnelseealiqilkepknaltkqyqalfnlegvdlefrdealdaiakk
amarktgarglrsiveaalldtmydlpsmedvekvvidesvidgqseplliyg

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >6wrfE (E:)
mgsshhhhhhdydipttenlyfqgssalptpheirnhlddyvigqeqakkvlavavynhy
krlrngdtsngvelgksnilligptgsgktllaetlarlldvpftmadattlteagyvge
dveniiqkllqksdydvqkaqrgivyideidkisrksdnpsitrdvsgegvqqallklie
gtvaavppqggrkhpqqeflqvdtskilficggafagldkvishrvetgsgigfgatvka
ksdkasegellaqvepedlikfglipefigrlpvvatlnelseealiqilkepknaltkq
yqalfnlegvdlefrdealdaiakkamarktgarglrsiveaalldtmydlpsmedvekv
videsvidgqseplliygkpeaqqasge

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfE (E:)
salptpheirnhlddyvigqeqakkvlavavynhykrlrngdtsngvelgksnilligpt
gsgktllaetlarlldvpftmadattlteagyvgedveniiqkllqksdydvqkaqrgiv
yideidkisrksdnpsitrdvsgegvqqallkliegtvaavppqggrkhpqqeflqvdts
kilficggafagldkvishrvetgsgigfgatvksegellaqvepedlikfglipefigr
lpvvatlnelseealiqilkepknaltkqyqalfnlegvdlefrdealdaiakkamarkt
garglrsiveaalldtmydlpsmedvekvvidesvidgqseplliyg

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >6wrfF (F:)
mgsshhhhhhdydipttenlyfqgssalptpheirnhlddyvigqeqakkvlavavynhy
krlrngdtsngvelgksnilligptgsgktllaetlarlldvpftmadattlteagyvge
dveniiqkllqksdydvqkaqrgivyideidkisrksdnpsitrdvsgegvqqallklie
gtvaavppqggrkhpqqeflqvdtskilficggafagldkvishrvetgsgigfgatvka
ksdkasegellaqvepedlikfglipefigrlpvvatlnelseealiqilkepknaltkq
yqalfnlegvdlefrdealdaiakkamarktgarglrsiveaalldtmydlpsmedvekv
videsvidgqseplliygkpeaqqasge

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfF (F:)
lptpheirnhlddyvigqeqakkvlavavynhykrlrngdtsngvelgksnilligptgs
gktllaetlarlldvpftmadattlteagyvgedveniiqkllqksdydvqkaqrgivyi
deidkisrgegvqqallkliegtvaavppqggrkhpqqeflqvdtskilficggafagld
kvishrvetgsgigfgatvkaksdkasegellaqvepedlikfglipefigrlpvvatln
elseealiqilkepknaltkqyqalfnlegvdlefrdealdaiakkamarktgarglrsi
veaalldtmydlpsmedvekvvidesvidgqseplliyg

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >6wrfH (H:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrnenlyfqslehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfH (H:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrn

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >6wrfI (I:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrnenlyfqslehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfI (I:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrn

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >6wrfJ (J:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrnenlyfqslehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfJ (J:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrn

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >6wrfK (K:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrnenlyfqslehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfK (K:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrn

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >6wrfL (L:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrnenlyfqslehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfL (L:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrn

  • Chain 'M':
    Sequence, based on SEQRES records: (download) 
    >6wrfM (M:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrnenlyfqslehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfM (M:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrnenlyf

  • Chain 'N':
    Sequence, based on SEQRES records: (download) 
    >6wrfN (N:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthrnenlyfqslehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6wrfN (N:)
lvpmvieqtsrgersfdiysrllkervifltgqvedhmanlivaqmlfleaenpekdiyl
yinspggvitagmsiydtmqfikpdvsticmgqaasmgaflltagakgkrfclpnsrvmi
hqplggyqgqatdieihareilkvkgrmnelmalhtgqsleqierdterdrflsapeave
yglvdsilthr

  • Chain 'S':
    No sequence available.