PDB entry 6w1x

View 6w1x on RCSB PDB site
Description: cryo-em structure of anti-crispr acrif9, bound to the type i-f crrna- guided crispr surveillance complex
Deposited on 2020-03-04, released 2020-05-13
The last revision was dated 2020-06-17, with a file datestamp of 2020-06-12.
Experiment type: EM
Resolution: 3.9 Å
R-factor: N/A
AEROSPACI score: 0.07 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated protein Csy1
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: csy1, PA14_33330
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: Type I-F CRISPR-associated protein Csy2
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: csy2, ALP65_00953, EQH76_13810, FCG96_17775, PACL_0128
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: CRISPR-associated protein Csy3
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: EQH76_13805, FCG96_17770
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: CRISPR-associated protein Csy3
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: EQH76_13805, FCG96_17770
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: CRISPR-associated protein Csy3
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: EQH76_13805, FCG96_17770
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: CRISPR-associated protein Csy3
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: EQH76_13805, FCG96_17770
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: CRISPR-associated protein Csy3
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: EQH76_13805, FCG96_17770
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: CRISPR-associated protein Csy3
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: EQH76_13805, FCG96_17770
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: anti-CRISPR AcrIF9
    Species: Proteus penneri [TaxId:102862]
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: anti-CRISPR AcrIF9
    Species: Proteus penneri [TaxId:102862]
    Database cross-references and differences (RAF-indexed):
  • Chain 'L':
    Compound: CRISPR-associated endonuclease Cas6/Csy4
    Species: Pseudomonas aeruginosa [TaxId:287]
    Gene: cas6f, csy4, PA14_33300
    Database cross-references and differences (RAF-indexed):
  • Chain 'M':
    Compound: RNA (60-mer)
    Species: Pseudomonas aeruginosa [TaxId:287]

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6w1xA (A:)
mtsplptptwqelrqfiesfiqerlqgkldklqpdeddkrqtllathrreawladaarrv
gqlqlvthtlkpihpdargsnlhslpqapgqpglagshelgdrlvsdvvgnaaaldvfkf
lslqyqgknllnwltedsaealqalsdnaeqarewrqafigittvkgapashslakqlyf
plpgsgyhllaplfptslvhhvhallrearfgdaakaarearsrqeswphgfseypnlai
qkfggtkpqnisqlnnerrgenwllpslppnwqrqnvnapmrhssvfehdfgrtpevsrl
trtlqrflaktvhnnlairqrraqlvaqicdealqyaarlrelepgwsatpgcqlhdaeq
lwldplraqtdetflqrrlrgdwpaevgnrfanwlnravssdsqilgspeaaqwsqelsk
eltmfkeilederd

    Sequence, based on observed residues (ATOM records):
    >6w1xA (A:)
qlvthtlkpihpdargsnlhslpqapgqpglagshelgdrlvsdvvgapashslakqlyf
plpgsgyhllaplfptslvhhvhallrearfgdaakaarearsrqeswphgfseypnlai
qkfggtkpqnisqlnnerrgenwllpslppnwqrqnvnapmrhssvfehdfgrtpevsrl
trtlqrflaktvhnnlairqrraqlvaqicdealqyaarlrelepgwsatpgcqlhdaeq
lwldplraqtdetflqrrlrgdwpaevgnrfanwlnravssdsqilgspeaaqwsqelsk
eltmfkeilederd

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6w1xB (B:)
msvtdpeallllprlsiqnanaisspltwgfpspgaftgfvhalqrrvgisldieldgvg
ivchrfeaqisqpagkrtkvfnltrnplnrdgstaaiveegrahlevslllgvhgdgldd
hpaqeiarqvqeqagamrlaggsilpwcnerfpapnaellmlggsdeqrrknqrrltrrl
lpgfalvsreallqqhletlrttlpeattldalldlcrinfeppatsseeeasppdaawq
vrdkpgwlvpipagynalsplylpgevrnardretplrfvenlfglgewlsphrvaalsd
llwyhhaepdkglyrwstprfvehaia

    Sequence, based on observed residues (ATOM records):
    >6w1xB (B:)
vtdpeallllprlsiqnanaisspltwgfpspgaftgfvhalqrrvgisldieldgvgiv
chrfeaqisqpagkrtkvfnltrnplnrdgstaaiveegrahlevslllgvhgdglddhp
aqeiarqvqeqagamrlaggsilpwcnerfpapnaellmlggsdeqrrknqrrltrrllp
gfalvsreallqqhletlrttlpeattldalldlcrinfewqvrdkpgwlvpipagynal
splylpgevrnardretplrfvenlfglgewlsphrvaalsdllwyhhaepdkglyrwst
prfv

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6w1xC (C:)
mksshhhhhhenlyfqsnaskpilstasvlaferkldpsdalmsagawaqrdasqewpav
tvreksvrgtisnrlktkdrdpakldasiqspnlqtvdvanlpsdadtlkvrftlrvlgg
agtpsacndaayrdkllqtvatyvndqgfaelarryahnlanarflwrnrvgaeavevri
nhirqgevarawrfdalaiglrdfkadaeldalaeliasglsgsghvllevvafarigdg
qevfpsqelildkgdkkgqksktlysvrdaaaihsqkignalrtidtwypdedglgpiav
epygsvtsqgkayrqpkqkldfytlldnwvlrdeapaveqqhyvianlirggvfgeaeek

    Sequence, based on observed residues (ATOM records):
    >6w1xC (C:)
lstasvlaferkldpsdalmsagawaqrdasqewpavtvrekvdvanlpsdadtlkvrft
lrvlggagtpsacndaayrdkllqtvatyvndqgfaelarryahnlanarflwrnrvgae
avevrinhirqgevarawrfdalaiglrdfkadaeldalaeliasglsgsghvllevvaf
arigdgqevfpsqelktlysvrdaaaihsqkignalrtidtwypdedglgpiavepygsv
tsqgkayrqpkqkldfytlldnwvlrdeapaveqqhyvianlirggvfge

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6w1xD (D:)
mksshhhhhhenlyfqsnaskpilstasvlaferkldpsdalmsagawaqrdasqewpav
tvreksvrgtisnrlktkdrdpakldasiqspnlqtvdvanlpsdadtlkvrftlrvlgg
agtpsacndaayrdkllqtvatyvndqgfaelarryahnlanarflwrnrvgaeavevri
nhirqgevarawrfdalaiglrdfkadaeldalaeliasglsgsghvllevvafarigdg
qevfpsqelildkgdkkgqksktlysvrdaaaihsqkignalrtidtwypdedglgpiav
epygsvtsqgkayrqpkqkldfytlldnwvlrdeapaveqqhyvianlirggvfgeaeek

    Sequence, based on observed residues (ATOM records):
    >6w1xD (D:)
ilstasvlaferkldpsdalmsagawaqrdasqewpavtvreksvrgtisnrlktkdrdp
akldasiqspnlqtvdvanlpsdadtlkvrftlrvlggagtpsacndaayrdkllqtvat
yvndqgfaelarryahnlanarflwrnrvgaeavevrinhirqgevarawrfdalaiglr
dfkadaeldalaeliasglsgsghvllevvafarigdgqevfpsqelilgqksktlysvr
daaaihsqkignalrtidtwypdedglgpiavepygsvtsqgkayrqpkqkldfytlldn
wvlrdeapaveqqhyvianlirggvfge

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6w1xE (E:)
mksshhhhhhenlyfqsnaskpilstasvlaferkldpsdalmsagawaqrdasqewpav
tvreksvrgtisnrlktkdrdpakldasiqspnlqtvdvanlpsdadtlkvrftlrvlgg
agtpsacndaayrdkllqtvatyvndqgfaelarryahnlanarflwrnrvgaeavevri
nhirqgevarawrfdalaiglrdfkadaeldalaeliasglsgsghvllevvafarigdg
qevfpsqelildkgdkkgqksktlysvrdaaaihsqkignalrtidtwypdedglgpiav
epygsvtsqgkayrqpkqkldfytlldnwvlrdeapaveqqhyvianlirggvfgeaeek

    Sequence, based on observed residues (ATOM records):
    >6w1xE (E:)
ilstasvlaferkldpsdalmsagawaqrdasqewpavtvreksvrgtisnrlktkdrdp
akldasiqspnlqtvdvanlpsdadtlkvrftlrvlggagtpsacndaayrdkllqtvat
yvndqgfaelarryahnlanarflwrnrvgaeavevrinhirqgevarawrfdalaiglr
dfkadaeldalaeliasglsgsghvllevvafarigdgqevfpsqelilgqksktlysvr
daaaihsqkignalrtidtwypdedglgpiavepygsvtsqgkayrqpkqkldfytlldn
wvlrdeapaveqqhyvianlirggvfgea

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6w1xF (F:)
mksshhhhhhenlyfqsnaskpilstasvlaferkldpsdalmsagawaqrdasqewpav
tvreksvrgtisnrlktkdrdpakldasiqspnlqtvdvanlpsdadtlkvrftlrvlgg
agtpsacndaayrdkllqtvatyvndqgfaelarryahnlanarflwrnrvgaeavevri
nhirqgevarawrfdalaiglrdfkadaeldalaeliasglsgsghvllevvafarigdg
qevfpsqelildkgdkkgqksktlysvrdaaaihsqkignalrtidtwypdedglgpiav
epygsvtsqgkayrqpkqkldfytlldnwvlrdeapaveqqhyvianlirggvfgeaeek

    Sequence, based on observed residues (ATOM records):
    >6w1xF (F:)
ilstasvlaferkldpsdalmsagawaqrdasqewpavtvreksvrgtisnrlktkdrdp
akldasiqspnlqtvdvanlpsdadtlkvrftlrvlggagtpsacndaayrdkllqtvat
yvndqgfaelarryahnlanarflwrnrvgaeavevrinhirqgevarawrfdalaiglr
dfkadaeldalaeliasglsgsghvllevvafarigdgqevfpsqelildkgdkkgqksk
tlysvrdaaaihsqkignalrtidtwypdedglgpiavepygsvtsqgkayrqpkqkldf
ytlldnwvlrdeapaveqqhyvianlirggvfgea

  • Chain 'G':
    Sequence, based on SEQRES records:
    >6w1xG (G:)
mksshhhhhhenlyfqsnaskpilstasvlaferkldpsdalmsagawaqrdasqewpav
tvreksvrgtisnrlktkdrdpakldasiqspnlqtvdvanlpsdadtlkvrftlrvlgg
agtpsacndaayrdkllqtvatyvndqgfaelarryahnlanarflwrnrvgaeavevri
nhirqgevarawrfdalaiglrdfkadaeldalaeliasglsgsghvllevvafarigdg
qevfpsqelildkgdkkgqksktlysvrdaaaihsqkignalrtidtwypdedglgpiav
epygsvtsqgkayrqpkqkldfytlldnwvlrdeapaveqqhyvianlirggvfgeaeek

    Sequence, based on observed residues (ATOM records):
    >6w1xG (G:)
lstasvlaferkldpsdalmsagawaqrdasqewpavtvreksvrgtisnrlktkdrdpa
kldasiqspnlqtvdvanlpsdadtlkvrftlrvlggagtpsacndaayrdkllqtvaty
vndqgfaelarryahnlanarflwrnrvgaeavevrinhirqgevarawrfdalaiglrd
fkadaeldalaeliasglsgsghvllevvafarigdgqevfpsqelilqksktlysvrda
aaihsqkignalrtidtwypdedglgpiavepygsvtsqgkayrqpkqkldfytlldnwv
lrdeapaveqqhyvianlirggvfge

  • Chain 'H':
    Sequence, based on SEQRES records:
    >6w1xH (H:)
mksshhhhhhenlyfqsnaskpilstasvlaferkldpsdalmsagawaqrdasqewpav
tvreksvrgtisnrlktkdrdpakldasiqspnlqtvdvanlpsdadtlkvrftlrvlgg
agtpsacndaayrdkllqtvatyvndqgfaelarryahnlanarflwrnrvgaeavevri
nhirqgevarawrfdalaiglrdfkadaeldalaeliasglsgsghvllevvafarigdg
qevfpsqelildkgdkkgqksktlysvrdaaaihsqkignalrtidtwypdedglgpiav
epygsvtsqgkayrqpkqkldfytlldnwvlrdeapaveqqhyvianlirggvfgeaeek

    Sequence, based on observed residues (ATOM records):
    >6w1xH (H:)
lstasvlaferkldpsdalmsagawaqrdasqewpavtvreksvrgtisnrlktkdrdpa
kldasiqspnlqtvdvanlpsdadtlkvrftlrvlggagtpsacndaayrdkllqtvaty
vndqgfaelarryahnlanarflwrnrvgaeavevrinhirqgevarawrfdalaiglrd
fkadaeldalaeliasglsgsghvllevvafarigdgqevfpsqelildkgdkkgqkskt
lysvrdaaaihsqkignalrtidtwypdedglgpiavepygsvtsqgkayrqpkqkldfy
tlldnwvlrdeapaveqqhyvianlirggvfge

  • Chain 'I':
    Sequence, based on SEQRES records:
    >6w1xI (I:)
mkstyiikevqninsdregvkvettsltsakriasknqffhgtvlriesesgnwlayked
gkrwiece

    Sequence, based on observed residues (ATOM records):
    >6w1xI (I:)
styiikevqninsdregvkvettsltsakriasknqffhgtvlriesesgnwlaykedgk
rwie

  • Chain 'J':
    Sequence, based on SEQRES records:
    >6w1xJ (J:)
mkstyiikevqninsdregvkvettsltsakriasknqffhgtvlriesesgnwlayked
gkrwiece

    Sequence, based on observed residues (ATOM records):
    >6w1xJ (J:)
styiikevqninsdregvkvettsltsakriasknqffhgtvlriesesgnwlaykedgk
rwie

  • Chain 'L':
    Sequence; same for both SEQRES and ATOM records:
    >6w1xL (L:)
mdhyldirlrpdpefppaqlmsvlfgklhqalvaqggdrigvsfpdldesrsrlgerlri
hasaddlrallarpwleglrdhlqfgepavvphptpyrqvsrvqaksnperlrrrlmrrh
dlseeearkripdtvaraldlpfvtlrsqstgqhfrlfirhgplqvtaeeggftcyglsk
ggfvpwf

  • Chain 'M':
    No sequence available.