PDB entry 6v2c

View 6v2c on RCSB PDB site
Description: Complex of mutant (K162M) of E. coli L-asparaginase II with L-Asp. Covalent acyl-enzyme intermediate and tetrahedral intermediate
Class: hydrolase
Keywords: L-asparagine hydrolase, anti-cancer drug, catalytically deficient mutant, HYDROLASE
Deposited on 2019-11-22, released 2020-05-20
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-06-10, with a file datestamp of 2020-06-05.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • conflict (18)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6v2ca_
  • Chain 'B':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • expression tag (6)
      • conflict (18)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6v2cb1, d6v2cb2
  • Chain 'C':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • expression tag (6)
      • conflict (18)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6v2cc1, d6v2cc2
  • Chain 'D':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • expression tag (6)
      • conflict (18)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6v2cd1, d6v2cd2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6v2cA (A:)
mhhhhhhlpnitilatggdiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtmtnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6v2cA (A:)
lpnitilatggdiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqvvnigsqd
mndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvgamrpsts
msadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtmtnttdvatfksvnygplg
yihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgiv
sagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpq
karvllqlaltqtkdpqqiqqifnqy

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6v2cB (B:)
mhhhhhhlpnitilatggdiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtmtnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6v2cB (B:)
hlpnitilatggdiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqvvnigsq
dmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvgamrpst
smsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtmtnttdvatfksvnygpl
gyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgi
vsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnp
qkarvllqlaltqtkdpqqiqqifnqy

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6v2cC (C:)
mhhhhhhlpnitilatggdiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtmtnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6v2cC (C:)
hlpnitilatggdiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqvvnigsq
dmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvgamrpst
smsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtmtnttdvatfksvnygpl
gyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgi
vsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnp
qkarvllqlaltqtkdpqqiqqifnqy

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6v2cD (D:)
mhhhhhhlpnitilatggdiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtmtnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6v2cD (D:)
hlpnitilatggdiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqvvnigsq
dmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvgamrpst
smsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtmtnttdvatfksvnygpl
gyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgi
vsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnp
qkarvllqlaltqtkdpqqiqqifnqy