PDB entry 6uwg
View 6uwg on RCSB PDB site
Description: engineered variant of i-onui meganuclease with improved thermostability and e178d mutation at catalytic site
Deposited on
2019-11-05, released
2019-12-18
The last revision was dated
2020-07-22, with a file datestamp of
2020-07-17.
Experiment type: XRAY
Resolution: 2.22 Å
R-factor: N/A
AEROSPACI score: 0.34
(click here for full SPACI score report)
Chains and heterogens:
- Chain 'A':
Compound: I-OnuI-e-Therm-E178D
Species: synthetic construct, synthetic [TaxId:32630]
Database cross-references and differences (RAF-indexed):
- Chain 'B':
Compound: DNA (26-mer)
Species: synthetic construct, synthetic [TaxId:32630]
- Chain 'C':
Compound: DNA (26-mer)
Species: synthetic construct, synthetic [TaxId:32630]
- Heterogens: CA, EDO, HOH
PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.
- Chain 'A':
Sequence, based on SEQRES records:
>6uwgA (A:)
massrresinpwiltgfadaegsfllrirknnkssvgystelgfqitlhnkdksileniq
stwgvgviansgdnavslkvtrfedlkviidhfekyplitqkyadymlfkqafnvmenke
hltiegikelvrikaklnwgltdelkkafpeiiskerslinknipnfkwlagftsgdgcf
fvnliksksklgvqvqlvfsitqhirdknlmnslitylgcgyikkknksefswldfvvtk
fsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgr
vf
Sequence, based on observed residues (ATOM records):
>6uwgA (A:)
rresinpwiltgfadaegsfllrirknnkssvgystelgfqitlhnkdksileniqstwg
vgviansgdnavslkvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehlti
egikelvrikaklnwgltdelkkafpeiiskerslinknipnfkwlagftsgdgcffvnl
iksksklgvqvqlvfsitqhirdknlmnslitylgcgyikkknksefswldfvvtkfsdi
rdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgrvf
- Chain 'B':
No sequence available.
- Chain 'C':
No sequence available.