PDB entry 6uwg

View 6uwg on RCSB PDB site
Description: engineered variant of i-onui meganuclease with improved thermostability and e178d mutation at catalytic site
Deposited on 2019-11-05, released 2019-12-18
The last revision was dated 2020-07-22, with a file datestamp of 2020-07-17.
Experiment type: XRAY
Resolution: 2.22 Å
R-factor: N/A
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: I-OnuI-e-Therm-E178D
    Species: synthetic construct, synthetic [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 6UWG (Start-301)
  • Chain 'B':
    Compound: DNA (26-mer)
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'C':
    Compound: DNA (26-mer)
    Species: synthetic construct, synthetic [TaxId:32630]
  • Heterogens: CA, EDO, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6uwgA (A:)
    massrresinpwiltgfadaegsfllrirknnkssvgystelgfqitlhnkdksileniq
    stwgvgviansgdnavslkvtrfedlkviidhfekyplitqkyadymlfkqafnvmenke
    hltiegikelvrikaklnwgltdelkkafpeiiskerslinknipnfkwlagftsgdgcf
    fvnliksksklgvqvqlvfsitqhirdknlmnslitylgcgyikkknksefswldfvvtk
    fsdirdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgr
    vf
    

    Sequence, based on observed residues (ATOM records):
    >6uwgA (A:)
    rresinpwiltgfadaegsfllrirknnkssvgystelgfqitlhnkdksileniqstwg
    vgviansgdnavslkvtrfedlkviidhfekyplitqkyadymlfkqafnvmenkehlti
    egikelvrikaklnwgltdelkkafpeiiskerslinknipnfkwlagftsgdgcffvnl
    iksksklgvqvqlvfsitqhirdknlmnslitylgcgyikkknksefswldfvvtkfsdi
    rdkiipffqeytligtklkdfedwckvaklieekkhlteegldeikkiklnmnkgrvf
    

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.