PDB entry 6usf

View 6usf on RCSB PDB site
Description: cryoem structure of human alpha4beta2 nicotinic acetylcholine receptor with varenicline in complex with anti-bril synthetic antibody bak5
Deposited on 2019-10-26, released 2020-04-29
The last revision was dated 2020-07-29, with a file datestamp of 2020-07-04.
Experiment type: EM
Resolution: 3.87 Å
R-factor: N/A
AEROSPACI score: 0.11 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Chimera of soluble cytochrome b562 (BRIL) and neuronal acetylcholine receptor subunit alpha-4
    Species: Homo sapiens [TaxId:9606]
    Gene: CHRNA4, NACRA4
    Database cross-references and differences (RAF-indexed):
    • Uniprot P43681 (Start-331)
    • Uniprot P0ABE7 (342-442)
      • conflict (348)
      • linker (443-444)
    • Uniprot P43681
  • Chain 'B':
    Compound: Neuronal acetylcholine receptor subunit beta-2
    Species: Homo sapiens [TaxId:9606]
    Gene: CHRNB2
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: Neuronal acetylcholine receptor subunit beta-2
    Species: Homo sapiens [TaxId:9606]
    Gene: CHRNB2
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: Chimera of soluble cytochrome b562 (BRIL) and neuronal acetylcholine receptor subunit alpha-4
    Species: Homo sapiens [TaxId:9606]
    Gene: CHRNA4, NACRA4
    Database cross-references and differences (RAF-indexed):
    • Uniprot P43681 (Start-331)
    • Uniprot P0ABE7 (342-442)
      • conflict (348)
      • linker (443-444)
    • Uniprot P43681
  • Chain 'E':
    Compound: Neuronal acetylcholine receptor subunit beta-2
    Species: Homo sapiens [TaxId:9606]
    Gene: CHRNB2
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: Fab heavy chain
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • PDB 6USF (Start-228)
  • Chain 'K':
    Compound: Fab heavy chain
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • PDB 6USF (Start-228)
  • Chain 'L':
    Compound: Fab light chain
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • PDB 6USF
  • Chain 'M':
    Compound: Fab light chain
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • PDB 6USF
  • Heterogens: QMR, NAG, BMA

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6usfA (A:)
sshvetrahaeerllkklfsgynkwsrpvanisdvvlvrfglsiaqlidvdeknqmmttn
vwvkqewhdyklrwdpadyenvtsiripseliwrpdivlynnadgdfavthltkahlfhd
grvqwtppaiyksscsidvtffpfdqqnctmkfgswtydkakidlvnmhsrvdqldfwes
gewvivdavgtyntrkyeccaeiypdityafvirrlplfytinliipclliscltvlvfy
lpsecgekitlcisvllsltvfllliteiipstslvipligeyllftmifvtlsivitvf
vlnvhhrsprthtmptwvrrvfldivprlllmkrpsvvdtdfadlednwetlndnlkvie
kadnaaqvkdaltkmraaaldaqkatppkledkspdspemkdfrhgfdilvgqiddalkl
anegkvkeaqaaaeqlkttrnayiqkyedwkyvamvidriflwmfiivcllgtvglflpp
wlagmi

    Sequence, based on observed residues (ATOM records):
    >6usfA (A:)
etrahaeerllkklfsgynkwsrpvanisdvvlvrfglsiaqlidvdeknqmmttnvwvk
qewhdyklrwdpadyenvtsiripseliwrpdivlynnadgdfavthltkahlfhdgrvq
wtppaiyksscsidvtffpfdqqnctmkfgswtydkakidlvnmhsrvdqldfwesgewv
ivdavgtyntrkyeccaeiypdityafvirrlplfytinliipclliscltvlvfylpse
cgekitlcisvllsltvfllliteiipstslvipligeyllftmifvtlsivitvfvlnv
hhrsprthtmptwvrrvfldivprlllmadlednwetlndnlkviekadnaaqvkdaltk
mraaaldaqkatppkledkspdspemkdfrhgfdilvgqiddalklanegkvkeaqaaae
qlkttrnayiqdwkyvamvidriflwmfiivcllgtvglflppw

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6usfB (B:)
tdteerlvehlldpsrynklirpatngselvtvqlmvslaqlisvhereqimttnvwltq
ewedyrltwkpeefdnmkkvrlpskhiwlpdvvlynnadgmyevsfysnavvsydgsifw
lppaiyksackievkhfpfdqqnctmkfrswtydrteidlvlksevaslddftpsgewdi
valpgrrnenpddstyvditydfiirrkplfytinliipcvlitslailvfylpsdcgek
mtlcisvllaltvfllliskivpptsldvplvgkylmftmvlvtfsivtsvcvlnvhhrs
ptthtmapwvkvvfleklpallfmqqdddqsvsedwkyvamvidrlflwifvfvcvfgti
gmflqplfqnyttttflhsdhsapssksawshpqfek

    Sequence, based on observed residues (ATOM records):
    >6usfB (B:)
dteerlvehlldpsrynklirpatngselvtvqlmvslaqlisvhereqimttnvwltqe
wedyrltwkpeefdnmkkvrlpskhiwlpdvvlynnadgmyevsfysnavvsydgsifwl
ppaiyksackievkhfpfdqqnctmkfrswtydrteidlvlksevaslddftpsgewdiv
alpgrrnenpddstyvditydfiirrkplfytinliipcvlitslailvfylpsdcgekm
tlcisvllaltvfllliskivpptsldvplvgkylmftmvlvtfsivtsvcvlnvhhrsp
tthtmapwvkvvfleklpallfmqqsvsedwkyvamvidrlflwifvfvcvfgtigmf

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6usfC (C:)
tdteerlvehlldpsrynklirpatngselvtvqlmvslaqlisvhereqimttnvwltq
ewedyrltwkpeefdnmkkvrlpskhiwlpdvvlynnadgmyevsfysnavvsydgsifw
lppaiyksackievkhfpfdqqnctmkfrswtydrteidlvlksevaslddftpsgewdi
valpgrrnenpddstyvditydfiirrkplfytinliipcvlitslailvfylpsdcgek
mtlcisvllaltvfllliskivpptsldvplvgkylmftmvlvtfsivtsvcvlnvhhrs
ptthtmapwvkvvfleklpallfmqqdddqsvsedwkyvamvidrlflwifvfvcvfgti
gmflqplfqnyttttflhsdhsapssksawshpqfek

    Sequence, based on observed residues (ATOM records):
    >6usfC (C:)
dteerlvehlldpsrynklirpatngselvtvqlmvslaqlisvhereqimttnvwltqe
wedyrltwkpeefdnmkkvrlpskhiwlpdvvlynnadgmyevsfysnavvsydgsifwl
ppaiyksackievkhfpfdqqnctmkfrswtydrteidlvlksevaslddftpsgewdiv
alpgrrnenpddstyvditydfiirrkplfytinliipcvlitslailvfylpsdcgekm
tlcisvllaltvfllliskivpptsldvplvgkylmftmvlvtfsivtsvcvlnvhhrsp
tthtmapwvkvvfleklpallfmqsvsedwkyvamvidrlflwifvfvcvfgtigmf

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6usfD (D:)
sshvetrahaeerllkklfsgynkwsrpvanisdvvlvrfglsiaqlidvdeknqmmttn
vwvkqewhdyklrwdpadyenvtsiripseliwrpdivlynnadgdfavthltkahlfhd
grvqwtppaiyksscsidvtffpfdqqnctmkfgswtydkakidlvnmhsrvdqldfwes
gewvivdavgtyntrkyeccaeiypdityafvirrlplfytinliipclliscltvlvfy
lpsecgekitlcisvllsltvfllliteiipstslvipligeyllftmifvtlsivitvf
vlnvhhrsprthtmptwvrrvfldivprlllmkrpsvvdtdfadlednwetlndnlkvie
kadnaaqvkdaltkmraaaldaqkatppkledkspdspemkdfrhgfdilvgqiddalkl
anegkvkeaqaaaeqlkttrnayiqkyedwkyvamvidriflwmfiivcllgtvglflpp
wlagmi

    Sequence, based on observed residues (ATOM records):
    >6usfD (D:)
etrahaeerllkklfsgynkwsrpvanisdvvlvrfglsiaqlidvdeknqmmttnvwvk
qewhdyklrwdpadyenvtsiripseliwrpdivlynnadgdfavthltkahlfhdgrvq
wtppaiyksscsidvtffpfdqqnctmkfgswtydkakidlvnmhsrvdqldfwesgewv
ivdavgtyntrkyeccaeiypdityafvirrlplfytinliipclliscltvlvfylpse
cgekitlcisvllsltvfllliteiipstslvipligeyllftmifvtlsivitvfvlnv
hhrsprthtmptwvrrvfldivprlllmadlednwetlndnlkviekadnaaqvkdaltk
mraaaldaqkatppkledkspdspemkdfrhgfdilvgqiddalklanegkvkeaqaaae
qlkttrnayiqdwkyvamvidriflwmfiivcllgtvglflppw

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6usfE (E:)
tdteerlvehlldpsrynklirpatngselvtvqlmvslaqlisvhereqimttnvwltq
ewedyrltwkpeefdnmkkvrlpskhiwlpdvvlynnadgmyevsfysnavvsydgsifw
lppaiyksackievkhfpfdqqnctmkfrswtydrteidlvlksevaslddftpsgewdi
valpgrrnenpddstyvditydfiirrkplfytinliipcvlitslailvfylpsdcgek
mtlcisvllaltvfllliskivpptsldvplvgkylmftmvlvtfsivtsvcvlnvhhrs
ptthtmapwvkvvfleklpallfmqqdddqsvsedwkyvamvidrlflwifvfvcvfgti
gmflqplfqnyttttflhsdhsapssksawshpqfek

    Sequence, based on observed residues (ATOM records):
    >6usfE (E:)
dteerlvehlldpsrynklirpatngselvtvqlmvslaqlisvhereqimttnvwltqe
wedyrltwkpeefdnmkkvrlpskhiwlpdvvlynnadgmyevsfysnavvsydgsifwl
ppaiyksackievkhfpfdqqnctmkfrswtydrteidlvlksevaslddftpsgewdiv
alpgrrnenpddstyvditydfiirrkplfytinliipcvlitslailvfylpsdcgekm
tlcisvllaltvfllliskivpptsldvplvgkylmftmvlvtfsivtsvcvlnvhhrsp
tthtmapwvkvvfleklpallfmqsvsedwkyvamvidrlflwifvfvcvfgtigmf

  • Chain 'H':
    Sequence, based on SEQRES records:
    >6usfH (H:)
eisevqlvesggglvqpggslrlscaasgfnvrsfsihwvrqapgkglewvayissssgs
tsyadsvkgrftisadtskntaylqmnslraedtavyycarwgywpgepwwkafdywgqg
tlvtvssastkgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtf
pavlqssglyslssvvtvpssslgtqtyicnvnhkpsntkvdkkvepks

    Sequence, based on observed residues (ATOM records):
    >6usfH (H:)
sevqlvesggglvqpggslrlscaasgfnvrsfsihwvrqapgkglewvayissssgsts
yadsvkgrftisadtskntaylqmnslraedtavyycarwgywpgepwwkafdywgqgtl
kgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssgly
slssvvtvpssslgtqtyicnvnhkpsntkvdkkvepks

  • Chain 'K':
    Sequence, based on SEQRES records:
    >6usfK (K:)
eisevqlvesggglvqpggslrlscaasgfnvrsfsihwvrqapgkglewvayissssgs
tsyadsvkgrftisadtskntaylqmnslraedtavyycarwgywpgepwwkafdywgqg
tlvtvssastkgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtf
pavlqssglyslssvvtvpssslgtqtyicnvnhkpsntkvdkkvepks

    Sequence, based on observed residues (ATOM records):
    >6usfK (K:)
sevqlvesggglvqpggslrlscaasgfnvrsfsihwvrqapgkglewvayissssgsts
yadsvkgrftisadtskntaylqmnslraedtavyycarwgywpgepwwkafdywgqgtl
vtvssastkgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpa
vlqssglyslssvvtvpssslgtqtyicnvnhkpsntkvdkkvepks

  • Chain 'L':
    Sequence, based on SEQRES records:
    >6usfL (L:)
sdiqmtqspsslsasvgdrvtitcrasqsvssavawyqqkpgkapklliysasslysgvp
srfsgsrsgtdftltisslqpedfatyycqqylyyslvtfgqgtkveikrtvaapsvfif
ppsdsqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsst
ltlskadyekhkvyacevthqglsspvtksfnrge

    Sequence, based on observed residues (ATOM records):
    >6usfL (L:)
diqmtqspsslsasvgdrvtitcrasqsvssavawyqqkpgkapklliysasslysgvps
rfsgsrsgtdftltisslqpedfatyycqqylyyslvtfgqgtkveiaapsvfifppsds
qlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlsk
adyekhkvyacevthqglsspvtksfnrg

  • Chain 'M':
    Sequence, based on SEQRES records:
    >6usfM (M:)
sdiqmtqspsslsasvgdrvtitcrasqsvssavawyqqkpgkapklliysasslysgvp
srfsgsrsgtdftltisslqpedfatyycqqylyyslvtfgqgtkveikrtvaapsvfif
ppsdsqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsst
ltlskadyekhkvyacevthqglsspvtksfnrge

    Sequence, based on observed residues (ATOM records):
    >6usfM (M:)
diqmtqspsslsasvgdrvtitcrasqsvssavawyqqkpgkapklliysasslysgvps
rfsgsrsgtdftltisslqpedfatyycqqylyyslvtfgqgtkveikrtvaapsvfifp
psdsqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstl
tlskadyekhkvyacevthqglsspvtksfnrg