PDB entry 6t32

View 6t32 on RCSB PDB site
Description: Streptavidin variants harbouring an artificial organocatalyst based cofactor
Class: Biotin-binding protein
Keywords: artificial cofactor, streptavidin, catalyst, Biotin-binding protein
Deposited on 2019-10-10, released 2020-11-18
The last revision prior to the SCOPe 2.08 freeze date was dated 2021-05-12, with a file datestamp of 2021-05-07.
Experiment type: XRAY
Resolution: 1.75 Å
R-factor: N/A
AEROSPACI score: 0.46 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered mutation (46)
      • engineered mutation (48)
      • engineered mutation (111)
      • engineered mutation (113)
      • engineered mutation (118)
      • engineered mutation (120)
      • engineered mutation (123)
    Domains in SCOPe 2.08: d6t32a_
  • Chain 'B':
    Compound: streptavidin
    Species: Streptomyces avidinii [TaxId:1895]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22629
      • engineered mutation (46)
      • engineered mutation (48)
      • engineered mutation (111)
      • engineered mutation (113)
      • engineered mutation (118)
      • engineered mutation (120)
      • engineered mutation (123)
    Domains in SCOPe 2.08: d6t32b_
  • Heterogens: HL9, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6t32A (A:)
masmtggqqmgrdeagitgtwynqlgstfivtagadgaltgtyesaagkaesryvltgry
dsapatdgsgtalgwtvawknnyrnahsattwsgqyvggaearintqwlltigateangw
astyvghdtftkvkpsaasidaakkagvnngnpldavqq

    Sequence, based on observed residues (ATOM records): (download) 
    >6t32A (A:)
agitgtwynqlgstfivtagadgaltgtyesaagkaesryvltgrydsapatdgsgtalg
wtvawknnyrnahsattwsgqyvggaearintqwlltigateangwastyvghdtftkvk
p

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6t32B (B:)
masmtggqqmgrdeagitgtwynqlgstfivtagadgaltgtyesaagkaesryvltgry
dsapatdgsgtalgwtvawknnyrnahsattwsgqyvggaearintqwlltigateangw
astyvghdtftkvkpsaasidaakkagvnngnpldavqq

    Sequence, based on observed residues (ATOM records): (download) 
    >6t32B (B:)
agitgtwynqlgstfivtagadgaltgtyesaagkaesryvltgrydsapatdgsgtalg
wtvawknnyrnahsattwsgqyvggaearintqwlltigateangwastyvghdtftkvk
p