PDB entry 6sqs

View 6sqs on RCSB PDB site
Description: Crystal structure of cat phospho-Ser429 MDM2 RING domain bound to UbcH5B-Ub
Class: ligase
Keywords: MDM2, MDMX, E3, E2, ubiquitin ligase, ubiquitin, phosphorylation, LIGASE
Deposited on 2019-09-04, released 2020-05-06
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-05-13, with a file datestamp of 2020-05-08.
Experiment type: XRAY
Resolution: 1.83 Å
R-factor: N/A
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

• Chain 'A':
  Compound: E3 ubiquitin-protein ligase Mdm2
  Species: Felis catus [TaxId:9685]
  Gene: MDM2
  Database cross-references and differences (RAF-indexed):
  • Uniprot Q7YRZ8 (0-63)
    • engineered mutation (15)
• Chain 'B':
  Compound: ubiquitin-conjugating enzyme e2 d2
  Species: Homo sapiens [TaxId:9606]
  Gene: UBE2D2, PUBC1, UBC4, UBC5B, UBCH4, UBCH5B
  Database cross-references and differences (RAF-indexed):
  • Uniprot P62837 (0-145)
    • engineered mutation (20)
    • engineered mutation (83)
  Domains in SCOPe 2.08: d6sqsb_
• Chain 'C':
  Compound: Ubiquitin-40S ribosomal protein S27a
  Species: Homo sapiens [TaxId:9606]
  Gene: RPS27A
  Database cross-references and differences (RAF-indexed):
  • Uniprot J3QTR3 (1-76)
    • expression tag (0)
  Domains in SCOPe 2.08: d6sqsc1, d6sqsc2
• Chain 'D':
  Compound: E3 ubiquitin-protein ligase Mdm2
  Species: Felis catus [TaxId:9685]
  Gene: MDM2
  Database cross-references and differences (RAF-indexed):
  • Uniprot Q7YRZ8 (0-63)
    • engineered mutation (15)
• Chain 'E':
  Compound: ubiquitin-conjugating enzyme e2 d2
  Species: Homo sapiens [TaxId:9606]
  Gene: UBE2D2, PUBC1, UBC4, UBC5B, UBCH4, UBCH5B
  Database cross-references and differences (RAF-indexed):
  • Uniprot P62837 (0-145)
    • engineered mutation (20)
    • engineered mutation (83)
  Domains in SCOPe 2.08: d6sqse_
• Chain 'F':
  Compound: Ubiquitin-40S ribosomal protein S27a
  Species: Homo sapiens [TaxId:9606]
  Gene: RPS27A
  Database cross-references and differences (RAF-indexed):
  • Uniprot J3QTR3 (1-76)
    • expression tag (0)
  Domains in SCOPe 2.08: d6sqsf1, d6sqsf2
• Heterogens: SEP, ZN, HOH

PDB Chain Sequences:

• Chain 'A':
  No sequence available.
  
  
• Chain 'B':
  Sequence; same for both SEQRES and ATOM records: (download)
  

  >6sqsB (B:)
  alkrihkelndlardppaqcragpvgddmfhwqatimgpndspyqggvffltihfptdyp
  fkppkvafttriyhpninsngsikldilrsqwspaltiskvllsicsllcdpnpddplvp
  eiariyktdrekynriarewtqkyam
  

  
  
• Chain 'C':
  Sequence; same for both SEQRES and ATOM records: (download)
  

  >6sqsC (C:)
  smqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdy
  niqkestlhlvlrlrgg
  

  
  
• Chain 'D':
  No sequence available.
  
  
• Chain 'E':
  Sequence; same for both SEQRES and ATOM records: (download)
  

  >6sqsE (E:)
  alkrihkelndlardppaqcragpvgddmfhwqatimgpndspyqggvffltihfptdyp
  fkppkvafttriyhpninsngsikldilrsqwspaltiskvllsicsllcdpnpddplvp
  eiariyktdrekynriarewtqkyam
  

  
  
• Chain 'F':
  Sequence; same for both SEQRES and ATOM records: (download)
  

  >6sqsF (F:)
  smqifvktltgktitlevepsdtienvkakiqdkegippdqqrlifagkqledgrtlsdy
  niqkestlhlvlrlrgg