PDB entry 6sgr

View 6sgr on RCSB PDB site
Description: cryo-em structure of escherichia coli acrbz and darpin in saposin a- nanodisc with cardiolipin
Deposited on 2019-08-05, released 2020-05-13
The last revision was dated 2020-06-10, with a file datestamp of 2020-06-05.
Experiment type: EM
Resolution: 3.17 Å
R-factor: N/A
AEROSPACI score: 0.12 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Multidrug efflux pump subunit AcrB
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: acrB, acrE, b0462, JW0451
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: Multidrug efflux pump subunit AcrB
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: acrB, acrE, b0462, JW0451
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: Multidrug efflux pump subunit AcrB
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: acrB, acrE, b0462, JW0451
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: darpin
    Species: synthetic construct, synthetic [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 6SGR
  • Chain 'E':
    Compound: darpin
    Species: synthetic construct, synthetic [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 6SGR
  • Chain 'F':
    Compound: Multidrug efflux pump accessory protein AcrZ
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: acrZ, ybhT, b0762, JW5102
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Multidrug efflux pump accessory protein AcrZ
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: acrZ, ybhT, b0762, JW5102
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: Multidrug efflux pump accessory protein AcrZ
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: acrZ, ybhT, b0762, JW5102
    Database cross-references and differences (RAF-indexed):

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6sgrA (A:)
mpnffidrpifawviaiiimlagglailklpvaqyptiappavtisasypgadaktvqdt
vtqvieqnmngidnlmymssnsdstgtvqitltfesgtdadiaqvqvqnklqlampllpq
evqqqgvsvekssssflmvvgvintdgtmtqedisdyvaanmkdaisrtsgvgdvqlfgs
qyamriwmnpnelnkfqltpvdvitaikaqnaqvaagqlggtppvkgqqlnasiiaqtrl
tsteefgkillkvnqdgsrvllrdvakielggenydiiaefngqpasglgiklatganal
dtaaairaelakmepffpsglkivypydttpfvkisihevvktlveaiilvflvmylflq
nfratliptiavpvvllgtfavlaafgfsintltmfgmvlaigllvddaivvvenvervm
aeeglppkeatrksmgqiqgalvgiamvlsavfvpmaffggstgaiyrqfsitivsamal
svlvaliltpalcatmlkpiakgdhgegkkgffgwfnrmfeksthhytdsvggilrstgr
ylvlyliivvgmaylfvrlpssflpdedqgvfmtmvqlpagatqertqkvlnevthyylt
keknnvesvfavngfgfagrgqntgiafvslkdwadrpgeenkveaitmratrafsqikd
amvfafnlpaivelgtatgfdfelidqaglghekltqarnqllaeaakhpdmltsvrpng
ledtpqfkididqekaqalgvsindinttlgaawggsyvndfidrgrvkkvyvmseakyr
mlpddigdwyvraadgqmvpfsafsssrweygsprlerynglpsmeilgqaapgkstgea
melmeqlasklptgvgydwtgmsyqerlsgnqapslyaislivvflclaalyeswsipfs
vmlvvplgvigallaatfrgltndvyfqvgllttiglsaknailivefakdlmdkegkgl
ieatldavrmrlrpilmtslafilgvmplvistgagsgaqnavgtgvmggmvtatvlaif
fvpvffvvvrrrfsrknediehshtvdhh

    Sequence, based on observed residues (ATOM records):
    >6sgrA (A:)
mpnffidrpifawviaiiimlagglailklpvaqyptiappavtisasypgadaktvqdt
vtqvieqnmngidnlmymssnsdstgtvqitltfesgtdadiaqvqvqnklqlampllpq
evqqqgvsvekssssflmvvgvintdgtmtqedisdyvaanmkdaisrtsgvgdvqlfgs
qyamriwmnpnelnkfqltpvdvitaikaqnaqvaagqlggtppvkgqqlnasiiaqtrl
tsteefgkillkvnqdgsrvllrdvakielggenydiiaefngqpasglgiklatganal
dtaaairaelakmepffpsglkivypydttpfvkisihevvktlveaiilvflvmylflq
nfratliptiavpvvllgtfavlaafgfsintltmfgmvlaigllvddaivvvenvervm
aeeglppkeatrksmgqiqgalvgiamvlsavfvpmaffggstgaiyrqfsitivsamal
svlvaliltpalcatmlkpiakgdhgegkkgffgwfnrmfeksthhytdsvggilrstgr
ylvlyliivvgmaylfvrlpssflpdedqgvfmtmvqlpagatqertqkvlnevthyylt
keknnvesvfavngfgfagrgqntgiafvslkdwadrpgeenkveaitmratrafsqikd
amvfafnlpaivelgtatgfdfelidqaglghekltqarnqllaeaakhpdmltsvrpng
ledtpqfkididqekaqalgvsindinttlgaawggsyvndfidrgrvkkvyvmseakyr
mlpddigdwyvraadgqmvpfsafsssrweygsprlerynglpsmeilgqaapgkstgea
melmeqlasklptgvgydwtgmsyqerlsgnqapslyaislivvflclaalyeswsipfs
vmlvvplgvigallaatfrgltndvyfqvgllttiglsaknailivefakdlmdkegkgl
ieatldavrmrlrpilmtslafilgvmplvistgagsgaqnavgtgvmggmvtatvlaif
fvpvffvvvrrrfsrknediehsh

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6sgrB (B:)
mpnffidrpifawviaiiimlagglailklpvaqyptiappavtisasypgadaktvqdt
vtqvieqnmngidnlmymssnsdstgtvqitltfesgtdadiaqvqvqnklqlampllpq
evqqqgvsvekssssflmvvgvintdgtmtqedisdyvaanmkdaisrtsgvgdvqlfgs
qyamriwmnpnelnkfqltpvdvitaikaqnaqvaagqlggtppvkgqqlnasiiaqtrl
tsteefgkillkvnqdgsrvllrdvakielggenydiiaefngqpasglgiklatganal
dtaaairaelakmepffpsglkivypydttpfvkisihevvktlveaiilvflvmylflq
nfratliptiavpvvllgtfavlaafgfsintltmfgmvlaigllvddaivvvenvervm
aeeglppkeatrksmgqiqgalvgiamvlsavfvpmaffggstgaiyrqfsitivsamal
svlvaliltpalcatmlkpiakgdhgegkkgffgwfnrmfeksthhytdsvggilrstgr
ylvlyliivvgmaylfvrlpssflpdedqgvfmtmvqlpagatqertqkvlnevthyylt
keknnvesvfavngfgfagrgqntgiafvslkdwadrpgeenkveaitmratrafsqikd
amvfafnlpaivelgtatgfdfelidqaglghekltqarnqllaeaakhpdmltsvrpng
ledtpqfkididqekaqalgvsindinttlgaawggsyvndfidrgrvkkvyvmseakyr
mlpddigdwyvraadgqmvpfsafsssrweygsprlerynglpsmeilgqaapgkstgea
melmeqlasklptgvgydwtgmsyqerlsgnqapslyaislivvflclaalyeswsipfs
vmlvvplgvigallaatfrgltndvyfqvgllttiglsaknailivefakdlmdkegkgl
ieatldavrmrlrpilmtslafilgvmplvistgagsgaqnavgtgvmggmvtatvlaif
fvpvffvvvrrrfsrknediehshtvdhh

    Sequence, based on observed residues (ATOM records):
    >6sgrB (B:)
mpnffidrpifawviaiiimlagglailklpvaqyptiappavtisasypgadaktvqdt
vtqvieqnmngidnlmymssnsdstgtvqitltfesgtdadiaqvqvqnklqlampllpq
evqqqgvsvekssssflmvvgvintdgtmtqedisdyvaanmkdaisrtsgvgdvqlfgs
qyamriwmnpnelnkfqltpvdvitaikaqnaqvaagqlggtppvkgqqlnasiiaqtrl
tsteefgkillkvnqdgsrvllrdvakielggenydiiaefngqpasglgiklatganal
dtaaairaelakmepffpsglkivypydttpfvkisihevvktlveaiilvflvmylflq
nfratliptiavpvvllgtfavlaafgfsintltmfgmvlaigllvddaivvvenvervm
aeeglppkeatrksmgqiqgalvgiamvlsavfvpmaffggstgaiyrqfsitivsamal
svlvaliltpalcatmlkpiakgdhgegkkgffgwfnrmfeksthhytdsvggilrstgr
ylvlyliivvgmaylfvrlpssflpdedqgvfmtmvqlpagatqertqkvlnevthyylt
keknnvesvfavngfgfagrgqntgiafvslkdwadrpgeenkveaitmratrafsqikd
amvfafnlpaivelgtatgfdfelidqaglghekltqarnqllaeaakhpdmltsvrpng
ledtpqfkididqekaqalgvsindinttlgaawggsyvndfidrgrvkkvyvmseakyr
mlpddigdwyvraadgqmvpfsafsssrweygsprlerynglpsmeilgqaapgkstgea
melmeqlasklptgvgydwtgmsyqerlsgnqapslyaislivvflclaalyeswsipfs
vmlvvplgvigallaatfrgltndvyfqvgllttiglsaknailivefakdlmdkegkgl
ieatldavrmrlrpilmtslafilgvmplvistgagsgaqnavgtgvmggmvtatvlaif
fvpvffvvvrrrf

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6sgrC (C:)
mpnffidrpifawviaiiimlagglailklpvaqyptiappavtisasypgadaktvqdt
vtqvieqnmngidnlmymssnsdstgtvqitltfesgtdadiaqvqvqnklqlampllpq
evqqqgvsvekssssflmvvgvintdgtmtqedisdyvaanmkdaisrtsgvgdvqlfgs
qyamriwmnpnelnkfqltpvdvitaikaqnaqvaagqlggtppvkgqqlnasiiaqtrl
tsteefgkillkvnqdgsrvllrdvakielggenydiiaefngqpasglgiklatganal
dtaaairaelakmepffpsglkivypydttpfvkisihevvktlveaiilvflvmylflq
nfratliptiavpvvllgtfavlaafgfsintltmfgmvlaigllvddaivvvenvervm
aeeglppkeatrksmgqiqgalvgiamvlsavfvpmaffggstgaiyrqfsitivsamal
svlvaliltpalcatmlkpiakgdhgegkkgffgwfnrmfeksthhytdsvggilrstgr
ylvlyliivvgmaylfvrlpssflpdedqgvfmtmvqlpagatqertqkvlnevthyylt
keknnvesvfavngfgfagrgqntgiafvslkdwadrpgeenkveaitmratrafsqikd
amvfafnlpaivelgtatgfdfelidqaglghekltqarnqllaeaakhpdmltsvrpng
ledtpqfkididqekaqalgvsindinttlgaawggsyvndfidrgrvkkvyvmseakyr
mlpddigdwyvraadgqmvpfsafsssrweygsprlerynglpsmeilgqaapgkstgea
melmeqlasklptgvgydwtgmsyqerlsgnqapslyaislivvflclaalyeswsipfs
vmlvvplgvigallaatfrgltndvyfqvgllttiglsaknailivefakdlmdkegkgl
ieatldavrmrlrpilmtslafilgvmplvistgagsgaqnavgtgvmggmvtatvlaif
fvpvffvvvrrrfsrknediehshtvdhh

    Sequence, based on observed residues (ATOM records):
    >6sgrC (C:)
mpnffidrpifawviaiiimlagglailklpvaqyptiappavtisasypgadaktvqdt
vtqvieqnmngidnlmymssnsdstgtvqitltfesgtdadiaqvqvqnklqlampllpq
evqqqgvsvekssssflmvvgvintdgtmtqedisdyvaanmkdaisrtsgvgdvqlfgs
qyamriwmnpnelnkfqltpvdvitaikaqnaqvaagqlggtppvkgqqlnasiiaqtrl
tsteefgkillkvnqdgsrvllrdvakielggenydiiaefngqpasglgiklatganal
dtaaairaelakmepffpsglkivypydttpfvkisihevvktlveaiilvflvmylflq
nfratliptiavpvvllgtfavlaafgfsintltmfgmvlaigllvddaivvvenvervm
aeeglppkeatrksmgqiqgalvgiamvlsavfvpmaffggstgaiyrqfsitivsamal
svlvaliltpalcatmlkpiakgdhgegkkgffgwfnrmfeksthhytdsvggilrstgr
ylvlyliivvgmaylfvrlpssflpdedqgvfmtmvqlpagatqertqkvlnevthyylt
keknnvesvfavngfgfagrgqntgiafvslkdwadrpgeenkveaitmratrafsqikd
amvfafnlpaivelgtatgfdfelidqaglghekltqarnqllaeaakhpdmltsvrpng
ledtpqfkididqekaqalgvsindinttlgaawggsyvndfidrgrvkkvyvmseakyr
mlpddigdwyvraadgqmvpfsafsssrweygsprlerynglpsmeilgqaapgkstgea
melmeqlasklptgvgydwtgmsyqerlsgnqapslyaislivvflclaalyeswsipfs
vmlvvplgvigallaatfrgltndvyfqvgllttiglsaknailivefakdlmdkegkgl
ieatldavrmrlrpilmtslafilgvmplvistgagsgaqnavgtgvmggmvtatvlaif
fvpvffvvvrrrf

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6sgrD (D:)
mrgshhhhhhgsdlgkklleaaragrddevrilmangadvnaadvvgwtplhlaaywghl
eivevllkngadvnaydtlgstplhlaahfghleivevllkngadvnakddngitplhla
anrghleivevllkygadvnaqdkfgktafdisinngnedlaeilqkln

    Sequence, based on observed residues (ATOM records):
    >6sgrD (D:)
gsdlgkklleaaragrddevrilmangadvnaadvvgwtplhlaaywghleivevllkng
advnaydtlgstplhlaahfghleivevllkngadvnakddngitplhlaanrghleive
vllkygadvnaqdkfgktafdisinngnedlaeilq

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6sgrE (E:)
mrgshhhhhhgsdlgkklleaaragrddevrilmangadvnaadvvgwtplhlaaywghl
eivevllkngadvnaydtlgstplhlaahfghleivevllkngadvnakddngitplhla
anrghleivevllkygadvnaqdkfgktafdisinngnedlaeilqkln

    Sequence, based on observed residues (ATOM records):
    >6sgrE (E:)
gkklleaaragrddevrilmangadvnaadvvgwtplhlaaywghleivevllkngadvn
aydtlgstplhlaahfghleivevllkngadvnakddngitplhlaanrghleivevllk
ygadvnaqdkfgktafdisinngnedlaeilq

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6sgrF (F:)
mlellkslvfavimvpvvmaiilgliyglgevfnifsgvgkkdqpgqnh

    Sequence, based on observed residues (ATOM records):
    >6sgrF (F:)
mlellkslvfavimvpvvmaiilgliyglgevfnifs

  • Chain 'G':
    Sequence, based on SEQRES records:
    >6sgrG (G:)
mlellkslvfavimvpvvmaiilgliyglgevfnifsgvgkkdqpgqnh

    Sequence, based on observed residues (ATOM records):
    >6sgrG (G:)
mlellkslvfavimvpvvmaiilgliyglgevfnif

  • Chain 'H':
    Sequence, based on SEQRES records:
    >6sgrH (H:)
mlellkslvfavimvpvvmaiilgliyglgevfnifsgvgkkdqpgqnh

    Sequence, based on observed residues (ATOM records):
    >6sgrH (H:)
mlellkslvfavimvpvvmaiilgliyglgevfnifs