PDB entry 6rin

View 6rin on RCSB PDB site
Description: cryo-em structure of e. coli rna polymerase backtracked elongation complex bound to greb transcription factor
Deposited on 2019-04-24, released 2019-07-03
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 3.7 Å
R-factor: N/A
AEROSPACI score: 0.08 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: rpoA, pez, phs, sez, b3295, JW3257
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: rpoA, pez, phs, sez, b3295, JW3257
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: DNA-directed RNA polymerase subunit beta
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: DNA-directed RNA polymerase subunit beta'
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: rpoC, tabB, b3988, JW3951
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: DNA-directed RNA polymerase subunit omega
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: rpoZ, b3649, JW3624
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: Transcription elongation factor greB
    Species: Escherichia coli K12 [TaxId:83333]
    Gene: greB, A6581_04870, A6592_17860, A9819_19355, ACN81_28045, AJ318_12850, AM446_03280, AM465_06470, AW059_21840, AW106_06800, AWP75_19390, B1K96_23455, B7C53_02740, B9M99_05190, B9N33_11850, B9T59_15180, BB545_11775, BE963_07880, BEN53_20780, BER14_23830, BIQ87_19125, BIU72_15205, BIZ41_07270, BN17_33411, BTQ04_09175, BTQ06_11100, BWP17_00335, BZL69_06140, C1I57_22680, C2M16_11315, C4K41_11925, C4M78_15275, C5715_11665, C5P43_01370, C5P44_10555, C6986_21785, C6B13_13810, C7B02_12915, C7B06_17550, C7B07_17730, C7B08_15410, CCZ14_09505, CCZ17_12365, CDL37_28230, CIJ94_13670, COD30_08990, COD46_24585, CQP61_02655, CR538_01935, CR539_22285, CRD98_08015, CRE06_04505, CRM83_22430, CRT43_20450, CRT46_19780, CSB64_14420, CT143_16930, CT146_14880, CVH05_26415, CWM24_10205, CXB56_05285, D1900_14635, D2183_13810, D2F89_07605, D3I61_20125, D5F38_05860, D6X47_11590, D7K63_10550, D7Y10_09590, D9D33_12115, D9D43_04330, D9E30_17490, D9E34_05680, D9E35_12335, D9E49_05660, D9F02_06885, D9F12_05730, D9F17_12145, D9F21_10030, D9G11_13190, D9G22_12525, D9G42_17600, D9G48_02635, D9G53_04685, D9H12_12450, D9H53_09445, D9H70_16165, D9H94_02685, D9I20_11335, D9I47_16295, D9I52_07930, D9I82_17890, D9I88_04675, D9J03_03135, D9J39_10095, D9J44_13380, D9J46_06810, D9K10_10415, DD762_12135, DIV22_04250, DL545_02535, DL800_24380, DL925_02870, DLU27_04270, DNQ45_10270, DNR41_15135, DNX30_18525, DOT75_01925, DP258_06460, DQE83_21230, DQF57_08805, DQP22_03685, DS732_24825, DS966_15260, DTL90_06605, DTM10_13330, DTM25_09000, DTM45_15925, DU309_12225, DU321_12435, DU333_19320, EA140_12925, EAI36_16755, EB510_07630, EB553_06270, EB569_05025, EB595_04935, ECs4248, ED060_02600, ED098_03500, ED124_04345, ED287_06925, ED600_01330, ED648_05650, ED653_03405, ED658_07555, ED944_05965, EEO96_14735, EEP03_05760, EF364_04490, EFV06_08470, EGT48_13510, EGY17_06960, EIA08_11750, EIA21_13775, HMPREF3040_00520
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: non-template DNA
    Species: Escherichia coli, synthetic [TaxId:562]
  • Chain 'R':
    Compound: RNA
    Species: Escherichia coli, synthetic [TaxId:562]
  • Chain 'T':
    Compound: Template DNA
    Species: Escherichia coli, synthetic [TaxId:562]
  • Heterogens: ZN, MG

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6rinA (A:)
mqgsvteflkprlvdieqvssthakvtleplergfghtlgnalrrillssmpgcavteve
idgvlheystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgd
veivkpqhvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspv
eriaynveaarveqrtdldklviemetngtidpeeairraatilaeqleafvdlrdvrqp
evkeekpefdpillrpvddleltvrsanclkaeaihyigdlvqrtevellktpnlgkksl
teikdvlasrglslgmrlenwppasiade

    Sequence, based on observed residues (ATOM records):
    >6rinA (A:)
eflkprlvdieqvssthakvtleplergfghtlgnalrrillssmpgcavteveidgvlh
eystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgdveivkp
qhvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspveriayn
veaarveqrtdldklviemetngtidpeeairraatilaeqleafvdl

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6rinB (B:)
mqgsvteflkprlvdieqvssthakvtleplergfghtlgnalrrillssmpgcavteve
idgvlheystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgd
veivkpqhvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspv
eriaynveaarveqrtdldklviemetngtidpeeairraatilaeqleafvdlrdvrqp
evkeekpefdpillrpvddleltvrsanclkaeaihyigdlvqrtevellktpnlgkksl
teikdvlasrglslgmrlenwppasiade

    Sequence, based on observed residues (ATOM records):
    >6rinB (B:)
svteflkprlvdieqvssthakvtleplergfghtlgnalrrillssmpgcavteveidg
vlheystkegvqedileillnlkglavrvqgkdeviltlnksgigpvtaadithdgdvei
vkpqhvichltdenasismrikvqrgrgyvpastrihseederpigrllvdacyspveri
aynveaarveqrtdldklviemetngtidpeeairraatilaeqleafv

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6rinC (C:)
mvysytekkrirkdfgkrpqvldvpyllsiqldsfqkfieqdpegqygleaafrsvfpiq
sysgnselqyvsyrlgepvfdvqecqirgvtysaplrvklrlviyereapegtvkdikeq
evymgeiplmtdngtfvingtervivsqlhrspgvffdsdkgkthssgkvlynariipyr
gswldfefdpkdnlfvridrrrklpatiilralnytteqildlffekvifeirdnklqme
lvperlrgetasfdieangkvyvekgrritarhirqlekddvklievpveyiagkvvakd
yidestgelicaanmelsldllaklsqsghkrietlftndldhgpyisetlrvdptndrl
salveiyrmmrpgepptreaaeslfenlffsedrydlsavgrmkfnrsllreeiegsgil
skddiidvmkklidirngkgevddidhlgnrrirsvgemaenqfrvglvrveravkerls
lgdldtlmpqdminakpisaavkeffgssqlsqfmdqnnplseithkrrisalgpggltr
eragfevrdvhpthygrvcpietpegpniglinslsvyaqtneygfletpyrkvtdgvvt
deihylsaieegnyviaqansnldeeghfvedlvtcrskgesslfsrdqvdymdvstqqv
vsvgaslipflehddanralmganmqrqavptlradkplvgtgmeravavdsgvtavakr
ggvvqyvdasrivikvnedemypgeagidiynltkytrsnqntcinqmpcvslgepverg
dvladgpstdlgelalgqnmrvafmpwngynfedsilvservvqedrfttihiqelacvs
rdtklgpeeitadipnvgeaalskldesgivyigaevtggdilvgkvtpkgetqltpeek
llraifgekasdvkdsslrvpngvsgtvidvqvftrdgvekdkraleieemqlkqakkdl
seelqileaglfsriravlvaggveaekldklprdrwlelgltdeekqnqleqlaeqyde
lkhefekkleakrrkitqgddlapgvlkivkvylavkrriqpgdkmagrhgnkgviskin
piedmpydengtpvdivlnplgvpsrmnigqilethlgmaakgigdkinamlkqqqevak
lrefiqraydlgadvrqkvdlstfsdeevmrlaenlrkgmpiatpvfdgakeaeikellk
lgdlptsgqirlydgrtgeqferpvtvgymymlklnhlvddkmharstgsyslvtqqplg
gkaqfggqrfgemevwaleaygaaytlqemltvksddvngrtkmyknivdgnhqmepgmp
esfnvllkeirslginielede

    Sequence, based on observed residues (ATOM records):
    >6rinC (C:)
vysytekkrirkdfgkrpqvldvpyllsiqldsfqkfieqdpegqygleaafrsvfpiqs
ysgnselqyvsyrlgepvfdvqecqirgvtysaplrvklrlviyereapegtvkdikeqe
vymgeiplmtdngtfvingtervivsqlhrspgvffdsdkgkthssgkvlynariipyrg
swldfefdpkdnlfvridrrrklpatiilralnytteqildlffekvifeirdnklqmel
vperlrgetasfdieangkvyvekgrritarhirqlekddvklievpveyiagkvvakdy
idestgelicaanmelsldllaklsqsghkrietlftndldhgpyisetlrvdptndrls
alveiyrmmrpgepptreaaeslfenlffsedrydlsavgrmkfnrsllreeiegsgils
kddiidvmkklidirngkgevddidhlgnrrirsvgemaenqfrvglvrveravkerlsl
gdldtlmpqdminakpisaavkeffgssqlsqfmdqnnplseithkrrisalgpggltre
ragfevrdvhpthygrvcpietpegpniglinslsvyaqtneygfletpyrkvtdgvvtd
eihylsaieegnyviaqansnldeeghfvedlvtcrskgesslfsrdqvdymdvstqqvv
svgaslipflehddanralmganmqrqavptlradkplvgtgmeravavdsgvtavakrg
gvvqyvdasrivikvnedemypgeagidiynltkytrsnqntcinqmpcvslgepvergd
vladgpstdlgelalgqnmrvafmpwngynfedsilvservvqedrfttihiqelacvsr
dtklgpeeitadipnvgeaalskldesgivyigaevtggdilvgkvtpkvkdsslrvpng
vsgtvidvqvftrdgvekdkraleieemqlkqakkdlseelqileaglfsriravlvagg
veaekldklprdrwlelgltdeekqnqleqlaeqydelkhefekkleakrrkitqgddla
pgvlkivkvylavkrriqpgdkmagrhgnkgviskinpiedmpydengtpvdivlnplgv
psrmnigqilethlgmaakgigdkinamlkqqqevaklrefiqraydlgadvrqkvdlst
fsdeevmrlaenlrkgmpiatpvfdgakeaeikellklgdlptsgqirlydgrtgeqfer
pvtvgymymlklnhlvddkmharstgsyslvtqqplggkaqfggqrfgemevwaleayga
aytlqemltvksddvngrtkmyknivdgnhqmepgmpesfnvllkeirslginielede

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6rinD (D:)
mkdllkflkaqtkteefdaikialaspdmirswsfgevkkpetinyrtfkperdglfcar
ifgpvkdyeclcgkykrlkhrgvicekcgvevtqtkvrrermghielasptahiwflksl
psrigllldmplrdiervlyfesyvvieggmtnlerqqilteeqyldaleefgdefdakm
gaeaiqallksmdleqeceqlreelnetnsetkrkkltkriklleafvqsgnkpewmilt
vlpvlppdlrplvpldggrfatsdlndlyrrvinrnnrlkrlldlaapdiivrnekrmlq
eavdalldngrrgraitgsnkrplksladmikgkqgrfrqnllgkrvdysgrsvitvgpy
lrlhqcglpkkmalelfkpfiygklelrglattikaakkmvereeavvwdildevirehp
vllnraptlhrlgiqafepvliegkaiqlhplvcaaynadfdgdqmavhvpltleaqlea
ralmmstnnilspangepiivpsqdvvlglyymtrdcvnakgegmvltgpkeaerlyrsg
laslharvkvriteyekdangelvaktslkdttvgrailwmivpkglpysivnqalgkka
iskmlntcyrilglkptvifadqimytgfayaarsgasvgiddmvipekkheiiseaeae
vaeiqeqfqsglvtagerynkvidiwaaandrvskammdnlqtetvinrdgqeekqvsfn
siymmadsgargsaaqirqlagmrglmakpdgsiietpitanfreglnvlqyfisthgar
kgladtalktansgyltrrlvdvaqdlvvteddcgthegimmtpvieggdvkeplrdrvl
grvtaedvlkpgtadilvprntllheqwcdlleensvdavkvrsvvscdtdfgvcahcyg
rdlarghiinkgeaigviaaqsigepgtqltmrtfhiggaasraaaessiqvknkgsikl
snvksvvnssgklvitsrntelklidefgrtkesykvpygavlakgdgeqvaggetvanw
dphtmpvitevsgfvrftdmidgqtitrqtdeltglsslvvldsaertaggkdlrpalki
vdaqgndvlipgtdmpaqyflpgkaivqledgvqissgdtlaripqesggtkditgglpr
vadlfearrpkepailaeisgivsfgketkgkrrlvitpvdgsdpyeemipkwrqlnvfe
gervergdvisdgpeaphdilrlrgvhavtryivnevqdvyrlqgvkindkhievivrqm
lrkativnagssdflegeqveysrvkianreleangkvgatysrdllgitkaslatesfi
saasfqettrvlteaavagkrdelrglkenvivgrlipagtgyayhqdrmrrraageapa
apqvtaedasaslaellnaglggsdne

    Sequence, based on observed residues (ATOM records):
    >6rinD (D:)
efdaikialaspdmirswsfgevkkpetinyrtfkperdglfcarifgpvkdyeclcgky
krlkhrgvicekcgvevtqtkvrrermghielasptahiwflkslpsrigllldmplrdi
ervlyfesyvvieggmtnlerqqilteeqyldaleefgdefdakmgaeaiqallksmdle
qeceqlreelnetnsetkrkkltkriklleafvqsgnkpewmiltvlpvlppdlrplvpl
dggrfatsdlndlyrrvinrnnrlkrlldlaapdiivrnekrmlqeavdalldngrrgra
itgsnkrplksladmikgkqgrfrqnllgkrvdysgrsvitvgpylrlhqcglpkkmale
lfkpfiygklelrglattikaakkmvereeavvwdildevirehpvllnraptlhrlgiq
afepvliegkaiqlhplvcaaynadfdgdqmavhvpltleaqlearalmmstnnilspan
gepiivpsqdvvlglyymtrdcvnakgegmvltgpkeaerlyrsglaslharvkvritey
ekdangelvaktslkdttvgrailwmivpkglpysivnqalgkkaiskmlntcyrilglk
ptvifadqimytgfayaarsgasvgiddmvipekkheiiseaeaevaeiqeqfqsglvta
gerynkvidiwaaandrvskammdnlqtetvinrdgqeekqvsfnsiymmadsgargsaa
qirqlagmrglmakpdgsiietpitanfreglnvlqyfisthgarkgladtalktansgy
ltrrlvdvaqdlvvteddcgthegimmtpvieggdvkeplrdrvlgrvtaedvlkpgtad
ilvprntllheqwcdlleensvdavkvrsvvscdtdfgvcahcygrdlarghiinkgeai
gviaaqsigepgtqltmrtfhiggaasraaaessiqvknkgsiklsnvksvvnssgklvi
tsrntelklidefgrtkesykvpygavlakgdgeqvaggetvanwdphtmpvitevsgfv
rftdmidgqtitrqtdeltglsslvvldsaertaggkdlrpalkivdaqgndvlipgtdm
paqyflpgkaivqledgvqissgdtlaripqesggtkditgglprvadlfearrpkepai
laeisgivsfgketkgkrrlvitpvdgsdpyeemipkwrqlnvfegervergdvisdgpe
aphdilrlrgvhavtryivnevqdvyrlqgvkindkhievivrqmlrkativnagssdfl
egeqveysrvkianreleangkvgatysrdllgitkaslatesfisaasfqettrvltea
avagkrdelrglkenvivgrlipagtgyayhqdrmrrr

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6rinE (E:)
marvtvqdavekignrfdlvlvaarrarqmqvggkdplvpeendkttvialreieeglin
nqildvrerqeqqeqeaaelqavtaiaegrr

    Sequence, based on observed residues (ATOM records):
    >6rinE (E:)
arvtvqdavekignrfdlvlvaarrarqmqvggkdplvpeendkttvialreieeglinn
qildvrerqeqqe

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6rinF (F:)
mktplvtregyeklkqelnylwreerpevtkkvtwaaslgdrsenadyqynkkrlreidr
rvryltkcmenlkivdyspqqegkvffgawveienddgvthrfrivgydeifgrkdyisi
dspmarallkkevgdlavvntpageaswyvnaieyvkp

    Sequence, based on observed residues (ATOM records):
    >6rinF (F:)
mktplvtregyeklkqelnylwreerpevtkkvtwaaslgdrsenadyqynkkrlreidr
rvryltkcmenlkivdyspqqegkvffgawveienddgvthrfrivgydeifgrkdyisi
dspmarallkkevgdlavvntpageaswyvnaieyv

  • Chain 'N':
    No sequence available.

  • Chain 'R':
    No sequence available.

  • Chain 'T':
    No sequence available.