PDB entry 6qxf

View 6qxf on RCSB PDB site
Description: cas1-cas2-csn2-dna complex from the type ii-a crispr-cas system
Deposited on 2019-03-07, released 2019-05-08
The last revision was dated 2019-07-24, with a file datestamp of 2019-07-19.
Experiment type: EM
Resolution: 3.6 Å
R-factor: N/A
AEROSPACI score: 0.1 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated protein Csn2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: csn2
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: CRISPR-associated protein Csn2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: csn2
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: CRISPR-associated protein Csn2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: csn2
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: CRISPR-associated protein Csn2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: csn2
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: CRISPR-associated protein Csn2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: csn2
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: CRISPR-associated protein Csn2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: csn2
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: CRISPR-associated protein Csn2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: csn2
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: CRISPR-associated protein Csn2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: csn2
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: CRISPR-associated endonuclease Cas1
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas1
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: CRISPR-associated endonuclease Cas1
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas1
    Database cross-references and differences (RAF-indexed):
  • Chain 'K':
    Compound: CRISPR-associated endoribonuclease Cas2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas2
    Database cross-references and differences (RAF-indexed):
  • Chain 'L':
    Compound: CRISPR-associated endonuclease Cas1
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas1
    Database cross-references and differences (RAF-indexed):
  • Chain 'M':
    Compound: CRISPR-associated endonuclease Cas1
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas1
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: CRISPR-associated endoribonuclease Cas2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas2
    Database cross-references and differences (RAF-indexed):
  • Chain 'O':
    Compound: CRISPR-associated endonuclease Cas1
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas1
    Database cross-references and differences (RAF-indexed):
  • Chain 'P':
    Compound: CRISPR-associated endonuclease Cas1
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas1
    Database cross-references and differences (RAF-indexed):
  • Chain 'Q':
    Compound: CRISPR-associated endoribonuclease Cas2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas2
    Database cross-references and differences (RAF-indexed):
  • Chain 'R':
    Compound: CRISPR-associated endonuclease Cas1
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas1
    Database cross-references and differences (RAF-indexed):
  • Chain 'S':
    Compound: CRISPR-associated endonuclease Cas1
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas1
    Database cross-references and differences (RAF-indexed):
  • Chain 'T':
    Compound: CRISPR-associated endoribonuclease Cas2
    Species: Streptococcus thermophilus [TaxId:1308]
    Gene: cas2
    Database cross-references and differences (RAF-indexed):
  • Chain 'X':
    Compound: DNA (25-mer)
    Species: Escherichia coli BL21(DE3) [TaxId:469008]
  • Chain 'Y':
    Compound: DNA (25-mer)
    Species: Escherichia coli BL21(DE3) [TaxId:469008]
  • Heterogens: CA

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6qxfA (A:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpynn

    Sequence, based on observed residues (ATOM records):
    >6qxfA (A:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpyn

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records:
    >6qxfB (B:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpynn

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6qxfC (C:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpynn

    Sequence, based on observed residues (ATOM records):
    >6qxfC (C:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpyn

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records:
    >6qxfD (D:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpynn

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6qxfE (E:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpynn

    Sequence, based on observed residues (ATOM records):
    >6qxfE (E:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpyn

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records:
    >6qxfF (F:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpynn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >6qxfG (G:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpynn

    Sequence, based on observed residues (ATOM records):
    >6qxfG (G:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpyn

  • Chain 'H':
    Sequence; same for both SEQRES and ATOM records:
    >6qxfH (H:)
mkinfslldepmevnlgtvlviedvsvfaqlvkefyqydeqsnltifdskirsirssell
litdilgydintsqvlkllhtdivsqlndkpevrseidslvslitdiimaecieneldie
ydeitllelikalgvrietksctvfekifeilqifkylvkkrilvfvnslsyfskdeiyq
ileytklsqadvlfleprqiegiqqfildkdyilmpynn

  • Chain 'I':
    Sequence, based on SEQRES records:
    >6qxfI (I:)
magwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvd
enilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyy
lgecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldyg
ytlllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvk
ikkelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefrilesgwshpqfe
ka

    Sequence, based on observed residues (ATOM records):
    >6qxfI (I:)
gwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvden
ilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyylg
ecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldygyt
lllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvkik
kelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefri

  • Chain 'J':
    Sequence, based on SEQRES records:
    >6qxfJ (J:)
magwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvd
enilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyy
lgecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldyg
ytlllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvk
ikkelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefrilesgwshpqfe
ka

    Sequence, based on observed residues (ATOM records):
    >6qxfJ (J:)
gwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvden
ilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyylg
ecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldygyt
lllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvkik
kelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefri

  • Chain 'K':
    Sequence, based on SEQRES records:
    >6qxfK (K:)
msyrymrmilmfdmptdtaeerkayrkfrkfllsegfimhqfsvysklllnhtantamvg
rlkannpkkgnitiltvtekqfarmiylygdkntsianseerlvflgdnycded

    Sequence, based on observed residues (ATOM records):
    >6qxfK (K:)
eerlvflgdny

  • Chain 'L':
    Sequence, based on SEQRES records:
    >6qxfL (L:)
magwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvd
enilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyy
lgecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldyg
ytlllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvk
ikkelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefrilesgwshpqfe
ka

    Sequence, based on observed residues (ATOM records):
    >6qxfL (L:)
gwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvden
ilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyylg
ecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldygyt
lllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvkik
kelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefri

  • Chain 'M':
    Sequence, based on SEQRES records:
    >6qxfM (M:)
magwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvd
enilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyy
lgecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldyg
ytlllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvk
ikkelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefrilesgwshpqfe
ka

    Sequence, based on observed residues (ATOM records):
    >6qxfM (M:)
gwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvden
ilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyylg
ecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldygyt
lllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvkik
kelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefri

  • Chain 'N':
    Sequence, based on SEQRES records:
    >6qxfN (N:)
msyrymrmilmfdmptdtaeerkayrkfrkfllsegfimhqfsvysklllnhtantamvg
rlkannpkkgnitiltvtekqfarmiylygdkntsianseerlvflgdnycded

    Sequence, based on observed residues (ATOM records):
    >6qxfN (N:)
eerlvflgdny

  • Chain 'O':
    Sequence, based on SEQRES records:
    >6qxfO (O:)
magwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvd
enilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyy
lgecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldyg
ytlllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvk
ikkelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefrilesgwshpqfe
ka

    Sequence, based on observed residues (ATOM records):
    >6qxfO (O:)
gwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvden
ilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyylg
ecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldygyt
lllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvkik
kelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefri

  • Chain 'P':
    Sequence, based on SEQRES records:
    >6qxfP (P:)
magwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvd
enilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyy
lgecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldyg
ytlllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvk
ikkelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefrilesgwshpqfe
ka

    Sequence, based on observed residues (ATOM records):
    >6qxfP (P:)
gwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvden
ilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyylg
ecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldygyt
lllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvkik
kelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefri

  • Chain 'Q':
    Sequence, based on SEQRES records:
    >6qxfQ (Q:)
msyrymrmilmfdmptdtaeerkayrkfrkfllsegfimhqfsvysklllnhtantamvg
rlkannpkkgnitiltvtekqfarmiylygdkntsianseerlvflgdnycded

    Sequence, based on observed residues (ATOM records):
    >6qxfQ (Q:)
eerlvflgdny

  • Chain 'R':
    Sequence, based on SEQRES records:
    >6qxfR (R:)
magwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvd
enilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyy
lgecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldyg
ytlllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvk
ikkelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefrilesgwshpqfe
ka

    Sequence, based on observed residues (ATOM records):
    >6qxfR (R:)
gwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvden
ilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyylg
ecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldygyt
lllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvkik
kelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefri

  • Chain 'S':
    Sequence, based on SEQRES records:
    >6qxfS (S:)
magwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvd
enilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyy
lgecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldyg
ytlllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvk
ikkelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefrilesgwshpqfe
ka

    Sequence, based on observed residues (ATOM records):
    >6qxfS (S:)
gwrtvvvnihsklsyknnhlifrnsyktemihlseidilllettdivlttmlvkrlvden
ilvifcddkrlptafltpyyarhdsslqiarqiawkenvkcevwtaiiaqkilnqsyylg
ecsffeksqsimelyhglerfdpsnreghsariyfntlfgndftresdndinaaldygyt
lllsmfarevvvcgcmtqiglkhanqfnqfnlasdimepfrpiidrivyqnrhnnfvkik
kelfsifsetylyngkemylsnivsdytkkvikalnqlgeeipefri

  • Chain 'T':
    Sequence, based on SEQRES records:
    >6qxfT (T:)
msyrymrmilmfdmptdtaeerkayrkfrkfllsegfimhqfsvysklllnhtantamvg
rlkannpkkgnitiltvtekqfarmiylygdkntsianseerlvflgdnycded

    Sequence, based on observed residues (ATOM records):
    >6qxfT (T:)
eerlvflgdny

  • Chain 'X':
    No sequence available.

  • Chain 'Y':
    No sequence available.