PDB entry 6pb0

View 6pb0 on RCSB PDB site
Description: cryo-em structure of urocortin 1-bound corticotropin-releasing factor 1 receptor in complex with gs protein and nb35
Deposited on 2019-06-12, released 2020-02-12
The last revision was dated 2020-02-19, with a file datestamp of 2020-02-14.
Experiment type: EM
Resolution: 3 Å
R-factor: N/A
AEROSPACI score: 0.16 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAS, GNAS1, GSP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63092
    • Uniprot P63096 (Start-188)
    • Uniprot P63092 (189-377)
      • engineered mutation (209)
      • engineered mutation (349)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: nanobody 35
    Species: synthetic construct, synthetic [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 6PB0
  • Chain 'R':
    Compound: Corticotropin-releasing factor receptor 1
    Species: Homo sapiens [TaxId:9606]
    Gene: CRHR1, CRFR, CRFR1, CRHR
    Database cross-references and differences (RAF-indexed):
  • Chain 'U':
    Compound: Urocortin
    Species: Homo sapiens [TaxId:9606]
    Gene: UCN
    Database cross-references and differences (RAF-indexed):
  • Heterogens: CLR, PLM

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6pb0A (A:)
mgclgnsktedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkstivkqm
rilhvngyseeeckqykavvysntiqsiiaiiramgrlkidfgdsaraddarqlfvlaga
aeegfmtaelagvikrlwkdsgvqacfnrsreyqlndsaayylndldriaqpnyiptqqd
vlrtrvkttgifetkfqvdkvnfhmfdvgaqrderrkwiqcfndvtaiifvvasssynmv
irednqtnrlqealnlfksiwnnrwlrtisvilflnkqdllaekvlagkskiedyfpefa
ryttpedatpepgedprvtrakyfirdeflristasgdgrhycyphftcsvdtenirrvf
ndcrdiiqrmhlrqyell

    Sequence, based on observed residues (ATOM records):
    >6pb0A (A:)
dqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkstivkqmtgifetkfqv
dkvnfhmfdvgaqrderrkwiqcfndvtaiifvvassqtnrlqealnlfksiwnnrwlrt
isvilflnkqdllaekvlakiedyfpefaryttpedatpepgedprvtrakyfirdeflr
istasgdgrhycyphftcsvdtenirrvfndcrdiiqrmhlrqyell

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6pb0B (B:)
mgsllqseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghl
akiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacg
gldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgq
qtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicff
pngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncn
vwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >6pb0B (B:)
eldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyamh
wgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnics
iynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttftg
htgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnafa
tgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalka
dragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >6pb0G (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records):
    >6pb0G (G:)
tasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'N':
    Sequence, based on SEQRES records:
    >6pb0N (N:)
maqvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasi
sytgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayr
gqgtqvtvsshhhhhhepea

    Sequence, based on observed residues (ATOM records):
    >6pb0N (N:)
qvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisy
tgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgq
gtqvtv

  • Chain 'R':
    Sequence, based on SEQRES records:
    >6pb0R (R:)
slqdqhceslslasnisglqcnasvdligtcwprspagqlvvrpcpaffygvrynttnng
yreclangswaarvnysecqeilneekkskvhyhvaviinylghcislvallvafvlflr
lrsirclrniihwnlisafilrnatwfvvqltmspevhqsnvgwcrlvtaaynyfhvtnf
fwmfgegcylhtaivltystdrlrkwmficigwgvpfpiivawaigklyydnekcwfgkr
pgvytdyiyqgpmilvllinfiflfnivrilmtklrasttsetiqyrkavkatlvllpll
gitymlffvnpgedevsrvvfiyfnsflesfqgffvsvfycflnsevrsairkrwhrwqd
khsirarvaramsip

    Sequence, based on observed residues (ATOM records):
    >6pb0R (R:)
lneekkskvhyhvaviinylghcislvallvafvlflrlrsirclrniihwnlisafilr
natwfvvqltmspevhqsnvgwcrlvtaaynyfhvtnffwmfgegcylhtaivltystdr
lrkwmficigwgvpfpiivawaigklyydnekcwfgkrpgvytdyiyqgpmilvllinfi
flfnivrilmtklrasttsetiqyrkavkatlvllpllgitymlffvnpgedevsrvvfi
yfnsflesfqgffvsvfycflnsevrsairkrwhrwqdkhs

  • Chain 'U':
    Sequence, based on SEQRES records:
    >6pb0U (U:)
dnpslsidltfhllrtllelartqsqreraeqnriifdsv

    Sequence, based on observed residues (ATOM records):
    >6pb0U (U:)
npslsidltfhllrtllelartqsqreraeqnriifdsv