PDB entry 6pa9

View 6pa9 on RCSB PDB site
Description: E. coli L-asparaginase II mutant (T12V) in complex with L-Asn at pH 7.0
Class: hydrolase
Keywords: inactive mutant, hydrolysis of L-asparagine, HYDROLASE
Deposited on 2019-06-11, released 2019-09-04
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-10-02, with a file datestamp of 2019-09-27.
Experiment type: XRAY
Resolution: 1.88 Å
R-factor: N/A
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (8-333)
      • engineered mutation (19)
    Domains in SCOPe 2.08: d6pa9a_
  • Chain 'B':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (8-333)
      • expression tag (7)
      • engineered mutation (19)
    Domains in SCOPe 2.08: d6pa9b1, d6pa9b2
  • Heterogens: ASN, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6pa9A (A:)
mdhhhhhhlpnitilatggviagggdsatksnytvgkvgvenlvnavpqlkdianvkgeq
vvnigsqdmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmv
gamrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtktnttdvatfk
svnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalv
dagydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfv
asgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6pa9A (A:)
lpnitilatggviagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqvvnigsqd
mndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvgamrpsts
msadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtktnttdvatfksvnygplg
yihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgiv
sagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpq
karvllqlaltqtkdpqqiqqifnqy

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6pa9B (B:)
mdhhhhhhlpnitilatggviagggdsatksnytvgkvgvenlvnavpqlkdianvkgeq
vvnigsqdmndnvwltlakkintdcdktdgfvithgtdtmeetayfldltvkcdkpvvmv
gamrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtktnttdvatfk
svnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalv
dagydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfv
asgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6pa9B (B:)
hlpnitilatggviavgvenlvnavpqlkdianvkgeqvvnigsqdmndnvwltlakkin
tdcdktdgfvithgtdtmeetayfldltvkcdkpvvmvgamrpstsmsadgpfnlynavv
taadkasanrgvlvvmndtvldgrdvtktnttdvatfksvnygplgyihngkidyqrtpa
rkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgngnlyksvf
dtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllqlaltqtk
dpqqiqqifnqy