PDB entry 6pa6

View 6pa6 on RCSB PDB site
Description: ECAII(T89V,K162T) MUTANT IN COMPLEX WITH L-ASN AT PH 8.3 in space group C2
Class: hydrolase
Keywords: inactive mutant, hydrolysis of L-asparagine, HYDROLASE
Deposited on 2019-06-11, released 2019-09-04
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-10-02, with a file datestamp of 2019-09-27.
Experiment type: XRAY
Resolution: 2.12 Å
R-factor: N/A
AEROSPACI score: 0.28 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (8-333)
      • engineered mutation (96)
      • engineered mutation (169)
    Domains in SCOPe 2.08: d6pa6a_
  • Chain 'B':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (8-333)
      • engineered mutation (96)
      • engineered mutation (169)
    Domains in SCOPe 2.08: d6pa6b_
  • Chain 'C':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (8-333)
      • engineered mutation (96)
      • engineered mutation (169)
    Domains in SCOPe 2.08: d6pa6c_
  • Chain 'D':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (8-333)
      • engineered mutation (96)
      • engineered mutation (169)
    Domains in SCOPe 2.08: d6pa6d_
  • Heterogens: ASN, IMD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6pa6A (A:)
mdhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeq
vvnigsqdmndnvwltlakkintdcdktdgfvithgvdtmeetayfldltvkcdkpvvmv
gamrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfk
svnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalv
dagydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfv
asgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6pa6A (A:)
lpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqvvnigsqd
mndnvwltlakkintdcdktdgfvithgvdtmeetayfldltvkcdkpvvmvgamrpsts
msadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplg
yihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgiv
sagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpq
karvllqlaltqtkdpqqiqqifnqy

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6pa6B (B:)
mdhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeq
vvnigsqdmndnvwltlakkintdcdktdgfvithgvdtmeetayfldltvkcdkpvvmv
gamrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfk
svnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalv
dagydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfv
asgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6pa6B (B:)
lpnitilatggtiagvgvenlvnavpqlkdianvkgeqvvnigsqdmndnvwltlakkin
tdcdktdgfvithgvdtmeetayfldltvkcdkpvvmvgamrpstsmsadgpfnlynavv
taadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplgyihngkidyqrtpa
rkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgngnlyksvf
dtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllqlaltqtk
dpqqiqqifnqy

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6pa6C (C:)
mdhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeq
vvnigsqdmndnvwltlakkintdcdktdgfvithgvdtmeetayfldltvkcdkpvvmv
gamrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfk
svnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalv
dagydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfv
asgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6pa6C (C:)
lpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqvvnigsqd
mndnvwltlakkintdcdktdgfvithgvdtmeetayfldltvkcdkpvvmvgamrpsts
msadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplg
yihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgiv
sagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpq
karvllqlaltqtkdpqqiqqifnqy

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6pa6D (D:)
mdhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeq
vvnigsqdmndnvwltlakkintdcdktdgfvithgvdtmeetayfldltvkcdkpvvmv
gamrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfk
svnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalv
dagydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfv
asgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6pa6D (D:)
lpnitilatggtiaggkvgvenlvnavpqlkdianvkgeqvvnigsqdmndnvwltlakk
intdcdktdgfvithgvdtmeetayfldltvkcdkpvvmvgamrpstsmsadgpfnlyna
vvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplgyihngkidyqrt
parkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgngnlyks
vfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllqlaltq
tkdpqqiqqifnqy