PDB entry 6p4b

View 6p4b on RCSB PDB site
Description: HyHEL10 fab variant HyHEL10-4x (heavy chain mutations L4F, Y33H, S56N, and Y58F) bound to hen egg lysozyme variant HEL2x-flex (mutations R21Q, R73E, C76S, and C94S)
Class: hydrolase/immune system
Keywords: HEL2x-flex, HyHEL10-4x, Fab, antigen-antibody, HYDROLASE-IMMUNE SYSTEM complex
Deposited on 2019-05-27, released 2020-05-27
The last revision prior to the SCOPe 2.07 freeze date was dated 2020-05-27, with a file datestamp of 2020-05-22.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: N/A
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: HyHEL10 Fab light chain
    Species: Mus musculus [TaxId:10090]
    Gene: LC
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d6p4ba1, d6p4ba2
  • Chain 'B':
    Compound: HyHEL10 Fab heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6P4B (0-End)
  • Chain 'C':
    Compound: Lysozyme C
    Species: Gallus gallus [TaxId:9031]
    Gene: LYZ
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00698 (0-128)
      • engineered mutation (20)
      • engineered mutation (72)
      • engineered mutation (75)
      • engineered mutation (93)
    Domains in SCOPe 2.07: d6p4bc_
  • Chain 'D':
    Compound: Lysozyme C
    Species: Gallus gallus [TaxId:9031]
    Gene: LYZ
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00698 (0-128)
      • engineered mutation (20)
      • engineered mutation (72)
      • engineered mutation (75)
      • engineered mutation (93)
    Domains in SCOPe 2.07: d6p4bd_
  • Chain 'H':
    Compound: HyHEL10 Fab heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6P4B (0-End)
  • Chain 'L':
    Compound: HyHEL10 Fab light chain
    Species: Mus musculus [TaxId:10090]
    Gene: LC
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d6p4bl1, d6p4bl2
  • Heterogens: CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >6p4bA (A:)
    divltqspatlsvtpgnsvslscrasqsignnlhwyqqkshesprllikyasqsisgips
    rfsgsgsgtdftlsinsvetedfgmyfcqqsnswpytfgggtkleikradaaptvsifpp
    sseqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstlt
    ltkdeyerhnsytceathktstspivksfnrnec
    

    Sequence, based on observed residues (ATOM records): (download)
    >6p4bA (A:)
    divltqspatlsvtpgnsvslscrasqsignnlhwyqqkshesprllikyasqsisgips
    rfsgsgsgtdftlsinsvetedfgmyfcqqsnswpytfgggtkleikradaaptvsifpp
    sseqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstlt
    ltkdeyerhnsytceathspivksfnrne
    

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download)
    >6p4bC (C:)
    kvfgrcelaaamkrhgldnyqgyslgnwvcaakfesnfntqatnrntdgstdygilqins
    rwwcndgrtpgsenlsnipcsallssditasvnsakkivsdgngmnawvawrnrckgtdv
    qawirgcrl
    

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download)
    >6p4bD (D:)
    kvfgrcelaaamkrhgldnyqgyslgnwvcaakfesnfntqatnrntdgstdygilqins
    rwwcndgrtpgsenlsnipcsallssditasvnsakkivsdgngmnawvawrnrckgtdv
    qawirgcrl
    

  • Chain 'H':
    No sequence available.

  • Chain 'L':
    Sequence, based on SEQRES records: (download)
    >6p4bL (L:)
    divltqspatlsvtpgnsvslscrasqsignnlhwyqqkshesprllikyasqsisgips
    rfsgsgsgtdftlsinsvetedfgmyfcqqsnswpytfgggtkleikradaaptvsifpp
    sseqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstlt
    ltkdeyerhnsytceathktstspivksfnrnec
    

    Sequence, based on observed residues (ATOM records): (download)
    >6p4bL (L:)
    divltqspatlsvtpgnsvslscrasqsignnlhwyqqkshesprllikyasqsisgips
    rfsgsgsgtdftlsinsvetedfgmyfcqqsnswpytfgggtkleikradaaptvsifpp
    sseqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstlt
    ltkdeyerhnsytceathkspivksfnrne