PDB entry 6ot0

View 6ot0 on RCSB PDB site
Description: structure of human smoothened-gi complex
Deposited on 2019-05-02, released 2019-06-12
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 3.9 Å
R-factor: N/A
AEROSPACI score: 0.04 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(i) subunit alpha-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAI1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63096 (0-353)
      • conflict (46)
      • conflict (202)
      • conflict (244)
      • conflict (325)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: Fab heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6OT0
  • Chain 'L':
    Compound: Fab light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6OT0
  • Chain 'R':
    Compound: Smoothened homolog
    Species: Homo sapiens [TaxId:9606]
    Gene: SMO, SMOH
    Database cross-references and differences (RAF-indexed):
  • Heterogens: CO1

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records:
    >6ot0A (A:)
mgctlsaedkaaverskmidrnlredgekaarevkllllgagesgkntivkqmkiiheag
yseeeckqykavvysntiqsiiaiiramgrlkidfgdsaraddarqlfvlagaaeegfmt
aelagvikrlwkdsgvqacfnrsreyqlndsaayylndldriaqpnyiptqqdvlrtrvk
ttgivethftfkdlhfkmfdvgaqrserkkwihcfegvtaiifcvalsdydlvlaedeem
nrmhasmklfdsicnnkwftdtsiilflnkkdlfeekikksplticypeyagsntyeeaa
ayiqcqfedlnkrkdtkeiythftcstdtknvqfvfdavtdviiknnlkdcglf

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6ot0B (B:)
mhhhhhhgsllqseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrr
tlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsg
nyvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalw
dietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdi
naicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagy
ddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >6ot0B (B:)
seldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyam
hwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnic
siynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttft
ghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnaf
atgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalk
adragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >6ot0G (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records):
    >6ot0G (G:)
ntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'H':
    Sequence, based on SEQRES records:
    >6ot0H (H:)
eisevqlvesggglvqpggslrlscaasgfnfyyssihwvrqapgkglewvasiysysgs
tsyadsvkgrftisadtskntaylqmnslraedtavyycarypwywwmekpylslygmdy
wgqgtlvtvssastkgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsg
vhtfpavlqssglyslssvvtvpssslgtqtyicnvnhkpsntkvdkkvepkscdktht

    Sequence, based on observed residues (ATOM records):
    >6ot0H (H:)
vqlvesggglvqpggslrlscaasgfnfyyssihwvrqapgkglewvasiysysgstsya
dsvkgrftisadtskntaylqmnslraedtavyycarypwywwmekpylslygmdywgqg
tlvtvs

  • Chain 'L':
    Sequence, based on SEQRES records:
    >6ot0L (L:)
sdiqmtqspsslsasvgdrvtitcrasqsvssavawyqqkpgkapklliysasslysgvp
srfsgsrsgtdftltisslqpedfatyycqqsssslitfgqgtkveikrtvaapsvfifp
psdsqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstl
tlskadyekhkvyacevthqglsspvtksfnrgec

    Sequence, based on observed residues (ATOM records):
    >6ot0L (L:)
mtqspsslsasvgdrvtitcrasqsvssavawyqqkpgkapklliysasslysgvpsrfs
gsrsgtdftltisslqpedfatyycqqsssslitfgqgtkveik

  • Chain 'R':
    Sequence, based on SEQRES records:
    >6ot0R (R:)
maaarpargpelpllglllllllgdpgrgaassgnatgpgprsaggsarrsaavtgpppp
lshcgraapceplrynvclgsvlpygatstllagdsdsqeeahgklvlwsglrnaprcwa
viqpllcavympkcendrvelpsrtlcqatrgpcaiverergwpdflrctpdrfpegctn
evqnikfnssgqcevplvrtdnpkswyedvegcgiqcqnplfteaehqdmhsyiaafgav
tglctlftlatfvadwrnsnrypavilfyvnacffvgsigwlaqfmdgarreivcradgt
mrlgeptsnetlscviifvivyyalmagvvwfvvltyawhtsfkalgttyqplsgktsyf
hlltwslpfvltvailavaqvdgdsvsgicfvgyknyryragfvlapiglvlivggyfli
rgvmtlfsiksnhpgllsekaaskinetmlrlgifgflafgfvlitfschfydffnqaew
ersfrdyvlcqanvtiglptkqpipdceiknrpsllvekinlfamfgtgiamstwvwtka
tlliwrrtwcrltgqdykddddk

    Sequence, based on observed residues (ATOM records):
    >6ot0R (R:)
sgqcevplvrtdnpkswyedvegcgiqcqnplfteaehqdmhsyiaafgavtglctlftl
atfvadwrnsnrypavilfyvnacffvgsigwlaqfmdgarreivcradgtmrlgeptsn
etlscviifvivyyalmagvvwfvvltyawhtsfkalgttyqplsgktsyfhlltwslpf
vltvailavaqvdgdsvsgicfvgyknyryragfvlapiglvlivggyflirgvmtlfsi
ksnhpgllsekaaskinetmlrlgifgflafgfvlitfschfydffnqaewersfrdyvl
cqanvtiglptkqpipdceiknrpsllvekinlfamfgtgiamstwvwtkatlliwrrtw
crlt