PDB entry 6nxc

View 6nxc on RCSB PDB site
Description: ecai(t162a) mutant in complex with citrate at ph 4
Class: hydrolase
Keywords: hydrolysis of L-asparagine, HYDROLASE
Deposited on 2019-02-08, released 2019-08-07
The last revision prior to the SCOPe 2.07 freeze date was dated 2019-08-07, with a file datestamp of 2019-08-02.
Experiment type: XRAY
Resolution: 1.74 Å
R-factor: N/A
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: L-asparaginase 1
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansA, b1767, JW1756
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A962 (Start-356)
      • engineered mutation (181)
    Domains in SCOPe 2.07: d6nxca_
  • Chain 'B':
    Compound: L-asparaginase 1
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansA, b1767, JW1756
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A962 (20-356)
      • expression tag (13-19)
      • engineered mutation (181)
    Domains in SCOPe 2.07: d6nxcb1, d6nxcb2
  • Chain 'C':
    Compound: L-asparaginase 1
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansA, b1767, JW1756
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A962 (Start-356)
      • engineered mutation (181)
    Domains in SCOPe 2.07: d6nxcc_
  • Chain 'D':
    Compound: L-asparaginase 1
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansA, b1767, JW1756
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0A962 (20-356)
      • expression tag (13-19)
      • engineered mutation (181)
    Domains in SCOPe 2.07: d6nxcd1, d6nxcd2
  • Heterogens: ASN, CIT, EDO, CL, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6nxcA (A:)
mgsshhhhhhssglvprgshmqkksiyvaytggtigmqrseqgyipvsghlqrqlalmpe
fhrpempdftiheytplmdssdmtpedwqhiaedikahyddydgfvilhgtdtmaytasa
lsfmlenlgkpvivtgsqiplaelrsdgqinllnalyvaanypinevtlffnnrlyrgnr
takahadgfdafaspnlpplleagihirrlntppaphgegelivhpitpqpigvvtiypg
isadvvrnflrqpvkalilrsygvgnapqnkaflqelqeasdrgivvvnltqcmsgkvnm
ggyatgnalahagviggadmtveatltklhyllsqeldtetirkamsqnlrgeltpd

    Sequence, based on observed residues (ATOM records): (download) 
    >6nxcA (A:)
kksiyvaytggtigmqrsipvsghlqrqlalmpefhrpempdftiheytplmdssdmtpe
dwqhiaedikahyddydgfvilhgtdtmaytasalsfmlenlgkpvivtgsqiplaelrs
dgqinllnalyvaanypinevtlffnnrlyrgnrtakahadgfdafaspnlpplleagih
irrlntppaphgegelivhpitpqpigvvtiypgisadvvrnflrqpvkalilrsygvgn
apqnkaflqelqeasdrgivvvnltqcmsgkvnmgnalahagviggadmtveatltklhy
llsqeldtetirkamsqnlrgeltpd

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6nxcB (B:)
mgsshhhhhhssglvprgshmqkksiyvaytggtigmqrseqgyipvsghlqrqlalmpe
fhrpempdftiheytplmdssdmtpedwqhiaedikahyddydgfvilhgtdtmaytasa
lsfmlenlgkpvivtgsqiplaelrsdgqinllnalyvaanypinevtlffnnrlyrgnr
takahadgfdafaspnlpplleagihirrlntppaphgegelivhpitpqpigvvtiypg
isadvvrnflrqpvkalilrsygvgnapqnkaflqelqeasdrgivvvnltqcmsgkvnm
ggyatgnalahagviggadmtveatltklhyllsqeldtetirkamsqnlrgeltpd

    Sequence, based on observed residues (ATOM records): (download) 
    >6nxcB (B:)
lvprgshmqkksiyvaytggtigmqrsghlqrqlalmpefhrpempdftiheytplmdss
dmtpedwqhiaedikahyddydgfvilhgtdtmaytasalsfmlenlgkpvivtgsqipl
aelrsdgqinllnalyvaanypinevtlffnnrlyrgnrtakahadgfdafaspnlppll
eagihirrlntppaphgegelivhpitpqpigvvtiypgisadvvrnflrqpvkalilrs
ygvgnapqnkaflqelqeasdrgivvvnltqcmsgkvnmggyatgnalahagviggadmt
veatltklhyllsqeldtetirkamsqnlrgeltpd

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6nxcC (C:)
mgsshhhhhhssglvprgshmqkksiyvaytggtigmqrseqgyipvsghlqrqlalmpe
fhrpempdftiheytplmdssdmtpedwqhiaedikahyddydgfvilhgtdtmaytasa
lsfmlenlgkpvivtgsqiplaelrsdgqinllnalyvaanypinevtlffnnrlyrgnr
takahadgfdafaspnlpplleagihirrlntppaphgegelivhpitpqpigvvtiypg
isadvvrnflrqpvkalilrsygvgnapqnkaflqelqeasdrgivvvnltqcmsgkvnm
ggyatgnalahagviggadmtveatltklhyllsqeldtetirkamsqnlrgeltpd

    Sequence, based on observed residues (ATOM records): (download) 
    >6nxcC (C:)
kksiyvaytggtigmqripvsghlqrqlalmpefhrpempdftiheytplmdssdmtped
wqhiaedikahyddydgfvilhgtdtmaytasalsfmlenlgkpvivtgsqiplaelrsd
gqinllnalyvaanypinevtlffnnrlyrgnrtakahadgfdafaspnlpplleagihi
rrlntppaphgegelivhpitpqpigvvtiypgisadvvrnflpvkalilrsygvgnapq
nkaflqelqeasdrgivvvnltqcmsgkvnmgnalahagviggadmtveatltklhylls
qeldtetirkamsqnlrgeltpd

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6nxcD (D:)
mgsshhhhhhssglvprgshmqkksiyvaytggtigmqrseqgyipvsghlqrqlalmpe
fhrpempdftiheytplmdssdmtpedwqhiaedikahyddydgfvilhgtdtmaytasa
lsfmlenlgkpvivtgsqiplaelrsdgqinllnalyvaanypinevtlffnnrlyrgnr
takahadgfdafaspnlpplleagihirrlntppaphgegelivhpitpqpigvvtiypg
isadvvrnflrqpvkalilrsygvgnapqnkaflqelqeasdrgivvvnltqcmsgkvnm
ggyatgnalahagviggadmtveatltklhyllsqeldtetirkamsqnlrgeltpd

    Sequence, based on observed residues (ATOM records): (download) 
    >6nxcD (D:)
lvprgshmqkksiyvaytggtigmqryipvsghlqrqlalmpefhrpempdftiheytpl
mdssdmtpedwqhiaedikahyddydgfvilhgtdtmaytasalsfmlenlgkpvivtgs
qiplaelrsdgqinllnalyvaanypinevtlffnnrlyrgnrtakahadgfdafaspnl
pplleagihirrlntppaphgegelivhpitpqpigvvtiypgisadvvrnflrqpvkal
ilrsygvgnapqnkaflqelqeasdrgivvvnltqcmsgkvnmnalahagviggadmtve
atltklhyllsqeldtetirkamsqnlrgeltpd