PDB entry 6nxa

View 6nxa on RCSB PDB site
Description: ecaii(d90t,k162t) mutant at ph 7
Class: hydrolase
Keywords: inactive mutant, hydrolysis of L-asparagine, HYDROLASE
Deposited on 2019-02-08, released 2019-08-07
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-08-19, with a file datestamp of 2020-08-14.
Experiment type: XRAY
Resolution: 1.93 Å
R-factor: N/A
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • expression tag (6)
      • conflict (96)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6nxaa1, d6nxaa2
  • Chain 'B':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • expression tag (6)
      • conflict (96)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6nxab1, d6nxab2
  • Chain 'C':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • expression tag (5-6)
      • conflict (96)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6nxac1, d6nxac2
  • Chain 'D':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • expression tag (5-6)
      • conflict (96)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6nxad1, d6nxad2
  • Heterogens: ACY, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6nxaA (A:)
mhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtttmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6nxaA (A:)
hlpnitilatggtiagnytvgkvgvenlvnavpqlkdianvkgeqvvnigsqdmndnvwl
tlakkintdcdktdgfvithgtttmeetayfldltvkcdkpvvmvgamrpstsmsadgpf
nlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplgyihngki
dyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgng
nlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllq
laltqtkdpqqiqqifnqy

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6nxaB (B:)
mhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtttmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6nxaB (B:)
hlpnitilatggtiagvenlvnavpqlkdianvkgeqvvnigsqdmndnvwltlakkint
dcdktdgfvithgtttmeetayfldltvkcdkpvvmvgamrpstsmsadgpfnlynavvt
aadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplgyihngkidyqrtpar
khtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgngnlyksvfd
tlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllqlaltqtkd
pqqiqqifnqy

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6nxaC (C:)
mhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtttmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6nxaC (C:)
hhlpnitilatggtiaggvenlvnavpqlkdianvkgeqvvnigsqdmndnvwltlakki
ntdcdktdgfvithgtttmeetayfldltvkcdkpvvmvgamrpstsmsadgpfnlynav
vtaadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplgyihngkidyqrtp
arkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgngnlyksv
fdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllqlaltqt
kdpqqiqqifnqy

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6nxaD (D:)
mhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtttmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6nxaD (D:)
hhlpnitilatggtiaggvenlvnavpqlkdianvkgeqvvnigsqdmndnvwltlakki
ntdcdktdgfvithgtttmeetayfldltvkcdkpvvmvgamrpstsmsadgpfnlynav
vtaadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplgyihngkidyqrtp
arkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgngnlyksv
fdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllqlaltqt
kdpqqiqqifnqy