PDB entry 6nx8

View 6nx8 on RCSB PDB site
Description: ecaii(d90t,k162t) mutant in complex with citrate at ph 6.2
Class: hydrolase
Keywords: inactive mutant, hydrolysis of L-asparagine, HYDROLASE
Deposited on 2019-02-08, released 2019-08-07
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-08-19, with a file datestamp of 2020-08-14.
Experiment type: XRAY
Resolution: 1.85 Å
R-factor: N/A
AEROSPACI score: 0.44 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • expression tag (5-6)
      • engineered mutation (96)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6nx8a1, d6nx8a2
  • Chain 'B':
    Compound: L-asparaginase 2
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: ansB, b2957, JW2924
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00805 (7-332)
      • engineered mutation (96)
      • engineered mutation (168)
    Domains in SCOPe 2.08: d6nx8b_
  • Heterogens: ACY, CIT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6nx8A (A:)
mhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtttmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6nx8A (A:)
hhlpnitilatggtiaggvenlvnavpqlkdianvkgeqvvnigsqdmndnvwltlakki
ntdcdktdgfvithgtttmeetayfldltvkcdkpvvmvgamrpstsmsadgpfnlynav
vtaadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplgyihngkidyqrtp
arkhtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgngnlyksv
fdtlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllqlaltqt
kdpqqiqqifnqy

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6nx8B (B:)
mhhhhhhlpnitilatggtiagggdsatksnytvgkvgvenlvnavpqlkdianvkgeqv
vnigsqdmndnvwltlakkintdcdktdgfvithgtttmeetayfldltvkcdkpvvmvg
amrpstsmsadgpfnlynavvtaadkasanrgvlvvmndtvldgrdvtttnttdvatfks
vnygplgyihngkidyqrtparkhtsdtpfdvsklnelpkvgivynyanasdlpakalvd
agydgivsagvgngnlyksvfdtlataaktgtavvrssrvptgattqdaevddakygfva
sgtlnpqkarvllqlaltqtkdpqqiqqifnqy

    Sequence, based on observed residues (ATOM records): (download) 
    >6nx8B (B:)
lpnitilatggtiaggvenlvnavpqlkdianvkgeqvvnigsqdmndnvwltlakkint
dcdktdgfvithgtttmeetayfldltvkcdkpvvmvgamrpstsmsadgpfnlynavvt
aadkasanrgvlvvmndtvldgrdvtttnttdvatfksvnygplgyihngkidyqrtpar
khtsdtpfdvsklnelpkvgivynyanasdlpakalvdagydgivsagvgngnlyksvfd
tlataaktgtavvrssrvptgattqdaevddakygfvasgtlnpqkarvllqlaltqtkd
pqqiqqifnqy