PDB entry 6nbi

View 6nbi on RCSB PDB site
Description: cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein
Deposited on 2018-12-07, released 2019-04-17
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 4 Å
R-factor: N/A
AEROSPACI score: 0.07 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Gs protein alpha subunit
    Species: Bos taurus [TaxId:9913]
    Database cross-references and differences (RAF-indexed):
    • PDB 6NBI (Start-377)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Rattus norvegicus [TaxId:10116]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Bos taurus [TaxId:9913]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'N':
    Compound: Nanobody-35
    Species: synthetic construct, synthetic [TaxId:32630]
    Database cross-references and differences (RAF-indexed):
    • PDB 6NBI (0-125)
  • Chain 'P':
    Compound: Long-acting parathyroid hormone analog
    Species: Homo sapiens [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
    • PDB 6NBI (0-End)
  • Chain 'R':
    Compound: Parathyroid hormone/parathyroid hormone-related peptide receptor
    Species: Homo sapiens [TaxId:9606]
    Gene: PTH1R, PTHR, PTHR1
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q03431
      • engineered mutation (161)
  • Heterogens: CLR

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6nbiA (A:)
mgclgnsktedqrneekaqreankkiekqlqkdkqvyrathrllllgagesgkstivkqm
rilhvngyseeeckqykavvysntiqsiiaiiramgrlkidfgdsaraddarqlfvlaga
aeegfmtaelagvikrlwkdsgvqacfnrsreyqlndsaayylndldriaqpnyiptqqd
vlrtrvkttgifetkfqvdkvnfhmfdvggqrderrkwiqcfndvtaiifvvasssynmv
irednqtnrlqealnlfksiwnnrwlrtisvilflnkqdllaekvlagkskiedyfpefa
ryttpedatpepgedprvtrakyfirdeflristasgdgrhycyphftcavdtenirrvf
ndcrdiiqrmhlrqyell

    Sequence, based on observed residues (ATOM records):
    >6nbiA (A:)
dqrneekaqreankkiekqlqkdkqvyrathrllllgagesgksttgifetkfqvdkvnf
hmfdvggqrderrkwiqcfndvtaiifvvassqtnrlqealnlfksiwnnrwlrtisvil
flnkqdllaekvlaiedyfpefaryttpedatpepgedprvtrakyfirdeflristasg
dgrhycyphftcavdtenirrvfndcrdiiqrmhlrqyell

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6nbiB (B:)
mgsllqseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghl
akiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacg
gldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgq
qtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicff
pngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncn
vwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >6nbiB (B:)
eldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrrtlrghlakiyamh
wgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsgnyvacggldnics
iynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalwdietgqqtttftg
htgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdinaicffpngnafa
tgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagyddfncnvwdalka
dragvlaghdnrvsclgvtddgmavatgswdsflkiwn

  • Chain 'G':
    Sequence, based on SEQRES records:
    >6nbiG (G:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records):
    >6nbiG (G:)
tasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenpfr

  • Chain 'N':
    Sequence; same for both SEQRES and ATOM records:
    >6nbiN (N:)
qvqlqesggglvqpggslrlscaasgftfsnykmnwvrqapgkglewvsdisqsgasisy
tgsvkgrftisrdnakntlylqmnslkpedtavyycarcpapftrdcfdvtsttyayrgq
gtqvtv

  • Chain 'P':
    Sequence, based on SEQRES records:
    >6nbiP (P:)
avaeiqlmhqrakwiqdarrraflhkliaeihtaei

    Sequence, based on observed residues (ATOM records):
    >6nbiP (P:)
avaeiqlmhqrakwiqdarrraflhkliae

  • Chain 'R':
    Sequence, based on SEQRES records:
    >6nbiR (R:)
daddvmtkeeqifllhraqaqcekrlkevlqrpasimesdkgwtsastsgkprkdkasgk
lypeseedkeaptgsryrgrpclpewdhilcwplgapgevvavpcpdyiydfnhkghayr
rcdrngswelvpghnrtwanysecvkfltnetrerevfdrlamiytvgysvslasltvav
lilayfrrlhctrnyihmhlflsfmlravsifvkdavlysgatldeaerlteeelraiaq
appppataaagyagcrvavtfflyflatnyywilveglylhslifmaffsekkylwgftv
fgwglpavfvavwvsvratlantgcwdlssgnkkwiiqvpilasivlnfilfinivrvla
tklretnagrcdtrqqyrkllkstlvlmplfgvhyivfmatpytevsgtlwqvqmhyeml
fnsfqgffvaiiycfcngevqaeikkswsrwtlaldfkrkarsgsssysygpmvshef

    Sequence, based on observed residues (ATOM records):
    >6nbiR (R:)
mtkeeqifllhraqaqcekrlkrpclpewdhilcwplgapgevvavpcpdyiydfnhkgh
ayrrcdrngsweltwanysecvkfltnetrerevfdrlamiytvgysvslasltvavlil
ayfrrlhctrnyihmhlflsfmlravsifvkdavlysgatltaaagyagcrvavtfflyf
latnyywilveglylhslifmaffsekkylwgftvfgwglpavfvavwvsvratlantgc
wdlssgnkkwiiqvpilasivlnfilfinivrvlatklretnagtrqqyrkllkstlvlm
plfgvhyivfmatpytevsgtlwqvqmhyemlfnsfqgffvaiiycfcngevqaeikksw
srwtlaldf