PDB entry 6n9w

View 6n9w on RCSB PDB site
Description: structure of bacteriophage t7 lagging-strand dna polymerase (d5a/e7a) and gp4 (helicase/primase) bound to dna including rna/dna hybrid, and an incoming dttp (lags2)
Deposited on 2018-12-04, released 2019-03-06
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 4 Å
R-factor: N/A
AEROSPACI score: 0.07 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA primase/helicase
    Species: Enterobacteria phage T7 [TaxId:10760]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03692
      • engineered mutation (342)
  • Chain 'B':
    Compound: DNA primase/helicase
    Species: Enterobacteria phage T7 [TaxId:10760]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03692
      • engineered mutation (342)
  • Chain 'C':
    Compound: DNA primase/helicase
    Species: Enterobacteria phage T7 [TaxId:10760]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03692
      • engineered mutation (342)
  • Chain 'D':
    Compound: DNA primase/helicase
    Species: Enterobacteria phage T7 [TaxId:10760]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03692
      • engineered mutation (342)
  • Chain 'E':
    Compound: DNA primase/helicase
    Species: Enterobacteria phage T7 [TaxId:10760]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P03692
      • engineered mutation (342)
  • Chain 'F':
    Compound: DNA primase/helicase
    Species: Enterobacteria phage T7 [TaxId:10760]
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: DNA-directed DNA polymerase
    Species: Enterobacteria phage T7 [TaxId:10760]
    Database cross-references and differences (RAF-indexed):
  • Chain 'P':
    Compound: Primer
    Species: Enterobacteria phage T7, synthetic [TaxId:10760]
  • Chain 'T':
    Compound: Template
    Species: Enterobacteria phage T7, synthetic [TaxId:10760]
  • Heterogens: ZN, TTP, MG

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6n9wA (A:)
mdnshdsdsvflyhipcdncgssdgnslfsdghtfcyvcekwtagnedtkeraskrkpsg
gkpmtynvwnfgesngrysaltargisketcqkagywiakvdgvmyqvadyrdqngnivs
qkvrdkdknfkttgshksdalfgkhlwnggkkivvtegeidmltvmelqdckypvvslgh
gasaakktcaanyeyfdqfeqiilmfdmdeagrkaveeaaqvlpagkvrvavlpckdane
chlnghdreimeqvwnagpwipdgvvsalslrerirehlsseesvgllfsgctgindktl
garggevimvtsgsgmgkstfvrqqalqwgtamgkkvglamlqesveetaedliglhnrv
rlrqsdslkreiiengkfdqwfdelfgndtfhlydsfaeaetdrllaklaymrsglgcdv
iildhisivvsasgesderkmidnlmtklkgfakstgvvlvvichlknpdkgkaheegrp
vsitdlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtgiagymeynketg
wlepssysgeeeshsestdwsndtdf

    Sequence, based on observed residues (ATOM records):
    >6n9wA (A:)
vflyhipcdncgssdgnslfsdghtfcyvcekwtamtynvwnfgesngrysaltargisk
etcqkagywiakvdgvmyqvadyrdqngnivsqkvrdkdknfkttgshksdalfgkhlwn
ggkkivvtegeidmltvmelqdckypvvslghgasaakktcaanyeyfdqfeqiilmfdm
aaqvlpagkvrvavlpckdanechlnghdreimeqvwnagpwipdgvvsalslrerireh
lsvgllfsgctgindktlgarggevimvtsgsgmgkstfvrqqalqwgtamgkkvglaml
qesveetaedliglhnrvrlrqsdslkreiiengkfdqwfdelfgndtfhlydstdrlla
klaymrsglgcdviildhisivvderkmidnlmtklkgfakstgvvlvvichlknpdkgk
aheegrpvsitdlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtgiagym
eynketgwlepssysg

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6n9wB (B:)
mdnshdsdsvflyhipcdncgssdgnslfsdghtfcyvcekwtagnedtkeraskrkpsg
gkpmtynvwnfgesngrysaltargisketcqkagywiakvdgvmyqvadyrdqngnivs
qkvrdkdknfkttgshksdalfgkhlwnggkkivvtegeidmltvmelqdckypvvslgh
gasaakktcaanyeyfdqfeqiilmfdmdeagrkaveeaaqvlpagkvrvavlpckdane
chlnghdreimeqvwnagpwipdgvvsalslrerirehlsseesvgllfsgctgindktl
garggevimvtsgsgmgkstfvrqqalqwgtamgkkvglamlqesveetaedliglhnrv
rlrqsdslkreiiengkfdqwfdelfgndtfhlydsfaeaetdrllaklaymrsglgcdv
iildhisivvsasgesderkmidnlmtklkgfakstgvvlvvichlknpdkgkaheegrp
vsitdlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtgiagymeynketg
wlepssysgeeeshsestdwsndtdf

    Sequence, based on observed residues (ATOM records):
    >6n9wB (B:)
tynvwnfgesngrysaltargisketcqkagywiakvdgvmyqvadyrdqngnivsqkvr
dkdknfkttgshksdalfgkhlwnggkkivvtegeidmltvmelqdckypvvslghgasa
akktcaanyeyfdqfeqiilmfdmdeagrkaveeaaqvlpagkvrvavlpckdanechln
ghdreimeqvwnagpwipdgvvsalslrerirehlsssvgllfsgctgindktlgargge
vimvtsgsgmgkstfvrqqalqwgtamgkkvglamlqesveetaedliglhnrvrlrqsd
slkreiiengkfdqwfdelfgndtfhlydstdrllaklaymrsglgcdviildhisivvs
sderkmidnlmtklkgfakstgvvlvvichlknpdkgkaheegrpvsitdlrgsgalrql
sdtiialernqqgdmpnlvlvrilkcrftgdtgiagymeynketgwlepssysg

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6n9wC (C:)
mdnshdsdsvflyhipcdncgssdgnslfsdghtfcyvcekwtagnedtkeraskrkpsg
gkpmtynvwnfgesngrysaltargisketcqkagywiakvdgvmyqvadyrdqngnivs
qkvrdkdknfkttgshksdalfgkhlwnggkkivvtegeidmltvmelqdckypvvslgh
gasaakktcaanyeyfdqfeqiilmfdmdeagrkaveeaaqvlpagkvrvavlpckdane
chlnghdreimeqvwnagpwipdgvvsalslrerirehlsseesvgllfsgctgindktl
garggevimvtsgsgmgkstfvrqqalqwgtamgkkvglamlqesveetaedliglhnrv
rlrqsdslkreiiengkfdqwfdelfgndtfhlydsfaeaetdrllaklaymrsglgcdv
iildhisivvsasgesderkmidnlmtklkgfakstgvvlvvichlknpdkgkaheegrp
vsitdlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtgiagymeynketg
wlepssysgeeeshsestdwsndtdf

    Sequence, based on observed residues (ATOM records):
    >6n9wC (C:)
dgvvsalslrerirehlsssvgllfsgctgindktlgarggevimvtsgsgmgkstfvrq
qalqwgtamgkkvglamlqesveetaedliglhnrvrlrqsdslkreiiengkfdqwfde
lfgndtfhlydsetdrllaklaymrsglgcdviildhisivvsasgesderkmidnlmtk
lkgfakstgvvlvvichlknpdkgkaheegrpvsitdlrgsgalrqlsdtiialernqmp
nlvlvrilkcrftgdtgiagymeynketgwlepssy

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6n9wD (D:)
mdnshdsdsvflyhipcdncgssdgnslfsdghtfcyvcekwtagnedtkeraskrkpsg
gkpmtynvwnfgesngrysaltargisketcqkagywiakvdgvmyqvadyrdqngnivs
qkvrdkdknfkttgshksdalfgkhlwnggkkivvtegeidmltvmelqdckypvvslgh
gasaakktcaanyeyfdqfeqiilmfdmdeagrkaveeaaqvlpagkvrvavlpckdane
chlnghdreimeqvwnagpwipdgvvsalslrerirehlsseesvgllfsgctgindktl
garggevimvtsgsgmgkstfvrqqalqwgtamgkkvglamlqesveetaedliglhnrv
rlrqsdslkreiiengkfdqwfdelfgndtfhlydsfaeaetdrllaklaymrsglgcdv
iildhisivvsasgesderkmidnlmtklkgfakstgvvlvvichlknpdkgkaheegrp
vsitdlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtgiagymeynketg
wlepssysgeeeshsestdwsndtdf

    Sequence, based on observed residues (ATOM records):
    >6n9wD (D:)
erirehlsvgllfsgctgindktlgarggevimvtsgsgmgkstfvrqqalqwgtamgkk
vglamlqesveetaedliglhnrvrlrqsdslkreiiengkfdqwfdelfgndtfhlyds
tdrllaklaymrsglgcdviildhisivvsrkmidnlmtklkgfakstgvvlvvichlkn
pdkgkaheegrpvsitdlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtg
iagymeynketgwlepssysg

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6n9wE (E:)
mdnshdsdsvflyhipcdncgssdgnslfsdghtfcyvcekwtagnedtkeraskrkpsg
gkpmtynvwnfgesngrysaltargisketcqkagywiakvdgvmyqvadyrdqngnivs
qkvrdkdknfkttgshksdalfgkhlwnggkkivvtegeidmltvmelqdckypvvslgh
gasaakktcaanyeyfdqfeqiilmfdmdeagrkaveeaaqvlpagkvrvavlpckdane
chlnghdreimeqvwnagpwipdgvvsalslrerirehlsseesvgllfsgctgindktl
garggevimvtsgsgmgkstfvrqqalqwgtamgkkvglamlqesveetaedliglhnrv
rlrqsdslkreiiengkfdqwfdelfgndtfhlydsfaeaetdrllaklaymrsglgcdv
iildhisivvsasgesderkmidnlmtklkgfakstgvvlvvichlknpdkgkaheegrp
vsitdlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtgiagymeynketg
wlepssysgeeeshsestdwsndtdf

    Sequence, based on observed residues (ATOM records):
    >6n9wE (E:)
vgllfsgctgindktlgarggevimvtsgsgmgkstfvrqqalqwgtamgkkvglamlqe
sveetaedliglhnrvrlrqsdslkreiikfdqwfdelfgndtfhlydrllaklaymrsg
lgcdviildhisivrkmidnlmtklkgfakstgvvlvvichlknpdkgkaheegrpvsit
dlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtgiagymeynketgwlep
s

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6n9wF (F:)
mdnshdsdsvflyhipcdncgssdgnslfsdghtfcyvcekwtagnedtkeraskrkpsg
gkpmtynvwnfgesngrysaltargisketcqkagywiakvdgvmyqvadyrdqngnivs
qkvrdkdknfkttgshksdalfgkhlwnggkkivvtegeidmltvmelqdckypvvslgh
gasaakktcaanyeyfdqfeqiilmfdmdeagrkaveeaaqvlpagkvrvavlpckdane
chlnghdreimeqvwnagpwipdgvvsalslrerirehlsseesvgllfsgctgindktl
garggevimvtsgsgmgkstfvrqqalqwgtamgkkvglamlqesveetaedliglhnrv
rlrqsdslkreiiengkfdqwfdelfgndtfhlydsfaeaetdrllaklaymrsglgcdv
iildhisivvsasgesderkmidnlmtklkgfakstgvvlvvichlknpdkgkaheegrp
vsitdlrgsgalrqlsdtiialernqqgdmpnlvlvrilkcrftgdtgiagymeynketg
wlepssysgeeeshsestdwsndtdf

    Sequence, based on observed residues (ATOM records):
    >6n9wF (F:)
gvvsalslrerirehls

  • Chain 'H':
    Sequence, based on SEQRES records:
    >6n9wH (H:)
mivsdieanallesvtkfhcgviydystaeyvsyrpsdfgayldaleaevargglivfhn
ghkydvpaltklaklqlnrefhlprencidtlvlsrlihsnlkdtdmgllrsgklpgkrf
gshaleawgyrlgemkgeykddfkrmleeqgeeyvdgmewwnfneemmdynvqdvvvtka
llekllsdkhyfppeidftdvgyttfwsesleavdiehraawllakqerngfpfdtkaie
elyvelaarrsellrkltetfgswyqpkggtemfchprtgkplpkypriktpkvggifkk
pknkaqregrepceldtreyvagapytpvehvvfnpssrdhiqkklqeagwvptkytdkg
apvvddevlegvrvddpekqaaidlikeylmiqkrigqsaegdkawlryvaedgkihgsv
npngavtgrathafpnlaqipgvrspygeqcraafgaehhldgitgkpwvqagidasgle
lrclahfmarfdngeyaheilngdihtknqiaaelptrdnaktfiygflygagdekigqi
vgagkergkelkkkflentpaiaalresiqqtlvessqwvageqqvkwkrrwikgldgrk
vhvrsphaalntllqsagalicklwiikteemlvekglkhgwdgdfaymawvhdeiqvgc
rteeiaqvvietaqeamrwvgdhwnfrclldtegkmgpnwaich

    Sequence, based on observed residues (ATOM records):
    >6n9wH (H:)
mivsdieanallesvtkfhcgviydystaeyvsyrpsdfgayldaleaevargglivfhn
ghkydvpaltklaklqlnrefhlprencidtlvlsrlihsnlkdtdmgllrklpgkrfgs
haleawgyrlgemkgeykddfkrmleeqgeeyvdgmewwnfneemmdynvqdvvvtkall
ekllsdkhyfppeidftdvgyttfwsesleavdiehraawllakqerngfpfdtkaieel
yvelaarrsellrkltetfgswyqpkytpvehvvfnpssrdhiqkklqeagwvptkytdk
gapvvddevlegvrvddpekqaaidlikeylmiqkrigqsaegdkawlryvaedgkihgs
vnpngavtgrathafpnlaqipgvrspygeqcraafgaehhldgitgkpwvqagidasgl
elrclahfmarfdngeyaheilngdihtknqiaaelptrdnaktfiygflygagdekigq
ivgagkergkelkkkflentpaiaalresiqqtlvessqwvageqqvkwkrrwikgldgr
kvhvrsphaalntllqsagalicklwiikteemlvekglkhgwdgdfaymawvhdeiqvg
crteeiaqvvietaqeamrwvgdhwnfrclldtegkmgpnwaich

  • Chain 'P':
    No sequence available.

  • Chain 'T':
    No sequence available.