PDB entry 6mus

View 6mus on RCSB PDB site
Description: cryo-em structure of larger csm-crrna-target rna ternary complex in type iii-a crispr-cas system
Deposited on 2018-10-23, released 2018-12-12
The last revision was dated 2019-01-30, with a file datestamp of 2019-01-25.
Experiment type: EM
Resolution: 3.6 Å
R-factor: N/A
AEROSPACI score: 0.09 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Uncharacterized protein Csm1
    Species: THERMOCOCCUS ONNURINEUS [TaxId:342948]
    Gene: TON_0893
    Database cross-references and differences (RAF-indexed):
    • Uniprot B6YWB8 (Start-790)
      • conflict (27-28)
  • Chain 'B':
    Compound: Uncharacterized protein Csm2
    Species: THERMOCOCCUS ONNURINEUS [TaxId:342948]
    Gene: TON_0894
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: Uncharacterized protein Csm3
    Species: THERMOCOCCUS ONNURINEUS [TaxId:342948]
    Gene: TON_0895
    Database cross-references and differences (RAF-indexed):
    • Uniprot B6YWC0
      • engineered mutation (36)
  • Chain 'D':
    Compound: Uncharacterized protein Csm3
    Species: THERMOCOCCUS ONNURINEUS [TaxId:342948]
    Gene: TON_0895
    Database cross-references and differences (RAF-indexed):
    • Uniprot B6YWC0 (1-End)
      • engineered mutation (36)
  • Chain 'E':
    Compound: Uncharacterized protein Csm4
    Species: THERMOCOCCUS ONNURINEUS [TaxId:342948]
    Gene: TON_0896
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: Uncharacterized protein Csm5
    Species: THERMOCOCCUS ONNURINEUS [TaxId:342948]
    Gene: TON_0897
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: RNA (33-mer)
    Species: Thermococcus onnurineus, synthetic [TaxId:342948]
  • Chain 'H':
    Compound: RNA (25-mer)
    Species: Thermococcus onnurineus, synthetic [TaxId:342948]
  • Chain 'J':
    Compound: Uncharacterized protein Csm2
    Species: THERMOCOCCUS ONNURINEUS [TaxId:342948]
    Gene: TON_0894
    Database cross-references and differences (RAF-indexed):
  • Chain 'K':
    Compound: Uncharacterized protein Csm3
    Species: THERMOCOCCUS ONNURINEUS [TaxId:342948]
    Gene: TON_0895
    Database cross-references and differences (RAF-indexed):
  • Heterogens: ZN

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6musA (A:)
mgsshhhhhhsqdpmeideltalggllanigkpvqraglysgdhstqgarflrdlaentg
raeyellslfsefhhkghmkndelmirrikelsperfgltmedvlnalwivyeadnlasg
ereegqpqasrplysvfnpgkaypwaeldfekelpvpgdvfsirsqdyrelvkrlweels
kaklrsdrllpvlekyltfvssvtsegniislydhmrmtsaialamlragctaedvrsgr
crkekrflliegdfsgiqdfiyrvsgkgtlkylrarsayleligwdvvleilsrlgltra
nvvfnagghfmiiaqntpdavkeleeirakavewlyrefesdlylaiewepvsgrefgre
ggknlfaearkrlkhkltvrklkrfgeikglfehghterlaecpvcgrelpegklepsas
dpetkvcptcnrlvslggnlpkllgfgrtakndagvlvegpfsgfvpylqggrpvgeqil
vkntlnpgeipesaqfvpyfvadyfkkdpkggvatfeelsmastgtrrlgvmkgdvdrlg
effssmdspsklatasrfmdyffkgyigaiiegkfgyiigdvpslrdwpeepdivvvyag
gddffivgawdqifelafrvrrafnaytggkltlsvglgyfdertpiyrmadvvserldt
akdegrnrvfvvgrsrpldgkhklsyewnhyeelwrtyapriyagngrlkgkleskkgll
wklleirelyvrdpndvrwayltayllgrhglsdlfpelvgidtkaverkepqpvywvdg
vlkivlmavrr

    Sequence, based on observed residues (ATOM records):
    >6musA (A:)
eideltalggllanigkpvqraglysgdhstqgarflrdlaentgraeyellslfsefhh
kghmkndelmirrikelsperfgltmedvlnalwivyeadnlasgerpqasrplysvfnp
gkaypwaeldfekelpvpgdvfsirsqdyrelvkrlweelskaklrsdrllpvlekyltf
vssvtsegniislydhmrmtsaialamlragctaedvrsgrcrkekrflliegdfsgiqd
fiyrvsgkgtlkylrarsayleligwdvvleilsrlgltranvvfnagghfmiiaqntpd
avkeleeirakavewlyrefesdlylaiewepvsgrefgreggknlfaearkrlkhkltv
rklkrfgeikglfehghterlaecpvcgrelpegklepsasdpetkvcptcnrlvslggn
lpkllgfgrtakndagvlvegpfsgfvpylqggrpvgeqilvkntlnpgeipesaqfvpy
fvadyfkkdpkggvatfeelsmastgtrrlgvmkgdvdrlgeffssmdspsklatasrfm
dyffkgyigaiiegkfgyiigdvpslrdwpeepdivvvyaggddffivgawdqifelafr
vrrafnaytggkltlsvglgyfdertpiyrmadvvserldtakdegrnrvfvvgrsrpld
gkhklsyewnhyeelwrtyapriyagngrlkgkleskkgllwklleirelyvrdpndvrw
ayltayllgrhglsdlfpelvgidtkaverkepqpvywvdgvlkivlmavrr

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6musB (B:)
smayhqkhggygrggygrqdrpqvdasrlfgespdvvgikkmlegkgkqweaiqpyfdnv
vreaknflewspnkrlanavtvaayltsqglktnqvrkildmarttelkvkrgegdikdd
lvkmryllaytvgkatgqskysldafhrildpmlevlmgspkkenfekfydflqavvayh
kffgggd

    Sequence, based on observed residues (ATOM records):
    >6musB (B:)
kgkqweaiqpyfdnvvreaknflewspnkrlanavtvaayltsqglktnqvrkildmart
telkvkrgegdikddlvkmryllaytvgkatgqskysldafhrildpmlevlmgspkken
fekfydflqavvayhkffggg

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6musC (C:)
smdrrfygkivikgkikavtglhigsqrdiseiggianpvikdphtglpyipgsslkgrl
rslfeilvnsrlgewrekypslanyspgscrpdnqencgkffnrkinrgwihvcpdyeta
lacpvcrlfgasgkesnfpsriivrdafltkeweekwrageaiteakievgidrvtsqan
prtnervvagaefefeiiynventthwrddiknlltamalledsylggsgsrgygkvkfi
fdsfefrpldyyrtgkdedivsidareksvsdilsgfdslfsevegkleag

    Sequence, based on observed residues (ATOM records):
    >6musC (C:)
rfygkivikgkikavtglhigsqrianpvikdphtglpyipgsslkgrlrslfeilvnsr
lgewrekypslanyspgscrpdnqencgkffnrkinrgwihvcpdyetalacpvcrlfga
sgkesnfpsriivrdafltkeweekwrageaiteakievgidrvtsqanprtnervvaga
efefeiiynventthwrddiknlltamalledsylggsgsrgygkvkfifdsfefrpldy
yrtgkdedivsidareksvsdilsgfdslfsevegkl

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6musD (D:)
smdrrfygkivikgkikavtglhigsqrdiseiggianpvikdphtglpyipgsslkgrl
rslfeilvnsrlgewrekypslanyspgscrpdnqencgkffnrkinrgwihvcpdyeta
lacpvcrlfgasgkesnfpsriivrdafltkeweekwrageaiteakievgidrvtsqan
prtnervvagaefefeiiynventthwrddiknlltamalledsylggsgsrgygkvkfi
fdsfefrpldyyrtgkdedivsidareksvsdilsgfdslfsevegkleag

    Sequence, based on observed residues (ATOM records):
    >6musD (D:)
mdrrfygkivikgkikavtglhigsqrdiseiggianpvikdphtglpyipgsslkgrlr
slfeilvnsrlgewrekypslanyspgscrpdnqencgkffnrkinrgwihvcpdyetal
acpvcrlfgasgkesnfpsriivrdafltkeweekwrageaiteakievgidrvtsqanp
rtnervvagaefefeiiynventthwrddiknlltamalledsylggsgsrgygkvkfif
dsfefrpldyyrtgkdedivsidareksvsdilsgfdslfsevegkl

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6musE (E:)
mpkfiavklipkgpfrdipradtlfgaignaisaihgqsaveelvdafvggarissafpy
sgdtyylpkplsvepalegiltgldeeeryttakrlrkakyldlknfelalrlrpftipe
eipyarvdvprvvldrvtqdssiyfweeirfreksgvyflysgprevfdgyiapamrflg
dtgiggkstwgaglfevefhemkidapgseysvtlsnalptktpvlwrllrkggwsfgrr
kprmtfiaegsivkndpggmerlelglshevyvygltfplgvelpegle

    Sequence, based on observed residues (ATOM records):
    >6musE (E:)
pkfiavklipkgpfrdipradtlfgaignaisaihgqsaveelvdafvggarissafpys
gdtyylpkplsvepalegilryttakrlrkakyldlknfelalrlrpftipeeipyarvd
vprvvldrvtqdssiyfweeirfreksgvyflysgprevfdgyiapamrflgdtgiggks
twgaglfevefhemkidapgseysvtlsnalptktpvlwrllrkggwsfgrrkprmtfia
egsivkndpggmerlelglshevyvygltfplgvelpeg

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6musF (F:)
mtertlkvlsplhigtgneltpvdiypreniihvldterlvndlmnlgvelneilallkn
ppgdayiwkgyieefhldpsdysiytlkihgkigrksmqikefiklngrpyipgsslkga
irtavlykalkecgdaravmrvvskvngdvardigrsedvldyymsflsraridrkradd
lleaivfgmepdrrskiryepkrdpmkalivrdskpvgrkhlavyhvevignpqpipiwv
eaiepgaatdveihvdtealrlnadyfngllweclkergepgevfedflweavdefytav
mkyetievqkfgrytsqvrsfyasledhsghvlrlgwgsgwlamtiglllvekgykwenv
rkklglgkkpggsgfsrefpktrrladgmpmgwvvlehhhhhh

    Sequence, based on observed residues (ATOM records):
    >6musF (F:)
mtertlkvlsplhigtgneltpvdiypreniihvldterlvndlmnlgelneilallknp
payiwkgyieefhldpsdysiytlkihgrksmqikefiklngrpyipgsslkgairtavl
ykalkecdaravmrvvskvngdvardigrdvldyymsflsrkraddlleaivfgmepdrr
skiryepkrdpmkalivrdskpvgrkhlavyhvevignpqpipiwveaiepgaatdveih
vdtealrlnadyfngllweclkergepgevfedflweavdefytavmkyetievqkfsqv
rsfyasledhsghvlrlgwgsgwlamtiglllvekgykwenvrkklglgkkgsgfsrefp
ktrrladgmpmgwvvle

  • Chain 'G':
    No sequence available.

  • Chain 'H':
    No sequence available.

  • Chain 'J':
    Sequence, based on SEQRES records:
    >6musJ (J:)
smayhqkhggygrggygrqdrpqvdasrlfgespdvvgikkmlegkgkqweaiqpyfdnv
vreaknflewspnkrlanavtvaayltsqglktnqvrkildmarttelkvkrgegdikdd
lvkmryllaytvgkatgqskysldafhrildpmlevlmgspkkenfekfydflqavvayh
kffgggd

    Sequence, based on observed residues (ATOM records):
    >6musJ (J:)
weaiqpyfdnvvreaknflewspnkrlanavtvaaktnqvrkildmarttelkikddlvk
mryllaytvgkatgqskysldafhrildpmlevlmgspkkenfekfydflqavvayhkff
g

  • Chain 'K':
    Sequence, based on SEQRES records:
    >6musK (K:)
smdrrfygkivikgkikavtglhigsqrdiseiggianpvikdphtglpyipgsslkgrl
rslfeilvnsrlgewrekypslanyspgscrpdnqencgkffnrkinrgwihvcpdyeta
lacpvcrlfgasgkesnfpsriivrdafltkeweekwrageaiteakievgidrvtsqan
prtnervvagaefefeiiynventthwrddiknlltamalledsylggsgsrgygkvkfi
fdsfefrpldyyrtgkdedivsidareksvsdilsgfdslfsevegkleag

    Sequence, based on observed residues (ATOM records):
    >6musK (K:)
mdrrfygkivikgkikavtglhignpvikdphtglpyipgsslkgrlrslfeilvnsrlg
ewrekypslanyspgscrpdnqencgkffnrkinrgwihvcpdyetalacpvcrlfgasg
kesnfpsriivrdafltkeweekwrageaiteakievgidrvtsqanprtnervvagaef
efeiiynventthwrddiknlltamalledsylggsgsrgygkvkfifdsfefrpldyyr
tgkdedivsidareksvsdilsgfdslfsevegkl