PDB entry 6mn5

View 6mn5 on RCSB PDB site
Description: crystal structure of aminoglycoside acetyltransferase aac(3)-iva, h154a mutant, in complex with gentamicin c1a
Deposited on 2018-10-01, released 2018-10-24
The last revision was dated 2022-04-06, with a file datestamp of 2022-04-01.
Experiment type: XRAY
Resolution: 2.58 Å
R-factor: N/A
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
    Species: Escherichia coli [TaxId:562]
    Gene: aacC4, aac(3), aac(3)-IV, aac(3)IV, aac3-VI, aac3-VI_1, aac3-VI_2, B9T59_28975, BEN53_26235, BJJ90_27515, BK373_27930, BK400_28640, BVL39_27730, BZL31_07055, BZL69_29365, C5P01_27985, C7235_00480, CR538_26885, CWS33_26775, CXB56_01995, pEC012_00026, SAMEA3472055_02859, SAMEA3472064_03809, SAMEA3472078_05206, SAMEA3472108_05065, SAMEA3472117_05674, SAMEA3472119_04576, SAMEA3472121_05663, SAMEA3485115_04534, SAMEA3751392_04817, SAMEA3752267_04833, SAMEA3752372_05888, SAMEA3752378_04585, SAMEA3752509_02517, SAMEA3752558_04925, SAMEA3752685_02521, SAMEA3753064_04967, SAMEA3753137_02787, SAMEA3753290_03667, SAMEA3753300_05389, SAMEA3753355_02348
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q306W4 (4-261)
      • expression tag (0-3)
      • engineered mutation (157)
  • Chain 'B':
    Compound: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
    Species: Escherichia coli [TaxId:562]
    Gene: aacC4, aac(3), aac(3)-IV, aac(3)IV, aac3-VI, aac3-VI_1, aac3-VI_2, B9T59_28975, BEN53_26235, BJJ90_27515, BK373_27930, BK400_28640, BVL39_27730, BZL31_07055, BZL69_29365, C5P01_27985, C7235_00480, CR538_26885, CWS33_26775, CXB56_01995, pEC012_00026, SAMEA3472055_02859, SAMEA3472064_03809, SAMEA3472078_05206, SAMEA3472108_05065, SAMEA3472117_05674, SAMEA3472119_04576, SAMEA3472121_05663, SAMEA3485115_04534, SAMEA3751392_04817, SAMEA3752267_04833, SAMEA3752372_05888, SAMEA3752378_04585, SAMEA3752509_02517, SAMEA3752558_04925, SAMEA3752685_02521, SAMEA3753064_04967, SAMEA3753137_02787, SAMEA3753290_03667, SAMEA3753300_05389, SAMEA3753355_02348
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q306W4 (4-261)
      • expression tag (0-3)
      • engineered mutation (157)
  • Chain 'C':
    Compound: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
    Species: Escherichia coli [TaxId:562]
    Gene: aacC4, aac(3), aac(3)-IV, aac(3)IV, aac3-VI, aac3-VI_1, aac3-VI_2, B9T59_28975, BEN53_26235, BJJ90_27515, BK373_27930, BK400_28640, BVL39_27730, BZL31_07055, BZL69_29365, C5P01_27985, C7235_00480, CR538_26885, CWS33_26775, CXB56_01995, pEC012_00026, SAMEA3472055_02859, SAMEA3472064_03809, SAMEA3472078_05206, SAMEA3472108_05065, SAMEA3472117_05674, SAMEA3472119_04576, SAMEA3472121_05663, SAMEA3485115_04534, SAMEA3751392_04817, SAMEA3752267_04833, SAMEA3752372_05888, SAMEA3752378_04585, SAMEA3752509_02517, SAMEA3752558_04925, SAMEA3752685_02521, SAMEA3753064_04967, SAMEA3753137_02787, SAMEA3753290_03667, SAMEA3753300_05389, SAMEA3753355_02348
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q306W4 (4-261)
      • expression tag (0-3)
      • engineered mutation (157)
  • Chain 'D':
    Compound: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
    Species: Escherichia coli [TaxId:562]
    Gene: aacC4, aac(3), aac(3)-IV, aac(3)IV, aac3-VI, aac3-VI_1, aac3-VI_2, B9T59_28975, BEN53_26235, BJJ90_27515, BK373_27930, BK400_28640, BVL39_27730, BZL31_07055, BZL69_29365, C5P01_27985, C7235_00480, CR538_26885, CWS33_26775, CXB56_01995, pEC012_00026, SAMEA3472055_02859, SAMEA3472064_03809, SAMEA3472078_05206, SAMEA3472108_05065, SAMEA3472117_05674, SAMEA3472119_04576, SAMEA3472121_05663, SAMEA3485115_04534, SAMEA3751392_04817, SAMEA3752267_04833, SAMEA3752372_05888, SAMEA3752378_04585, SAMEA3752509_02517, SAMEA3752558_04925, SAMEA3752685_02521, SAMEA3753064_04967, SAMEA3753137_02787, SAMEA3753290_03667, SAMEA3753300_05389, SAMEA3753355_02348
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q306W4 (4-261)
      • expression tag (0-3)
      • engineered mutation (157)
  • Chain 'E':
    Compound: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
    Species: Escherichia coli [TaxId:562]
    Gene: aacC4, aac(3), aac(3)-IV, aac(3)IV, aac3-VI, aac3-VI_1, aac3-VI_2, B9T59_28975, BEN53_26235, BJJ90_27515, BK373_27930, BK400_28640, BVL39_27730, BZL31_07055, BZL69_29365, C5P01_27985, C7235_00480, CR538_26885, CWS33_26775, CXB56_01995, pEC012_00026, SAMEA3472055_02859, SAMEA3472064_03809, SAMEA3472078_05206, SAMEA3472108_05065, SAMEA3472117_05674, SAMEA3472119_04576, SAMEA3472121_05663, SAMEA3485115_04534, SAMEA3751392_04817, SAMEA3752267_04833, SAMEA3752372_05888, SAMEA3752378_04585, SAMEA3752509_02517, SAMEA3752558_04925, SAMEA3752685_02521, SAMEA3753064_04967, SAMEA3753137_02787, SAMEA3753290_03667, SAMEA3753300_05389, SAMEA3753355_02348
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q306W4 (4-261)
      • expression tag (0-3)
      • engineered mutation (157)
  • Chain 'F':
    Compound: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
    Species: Escherichia coli [TaxId:562]
    Gene: aacC4, aac(3), aac(3)-IV, aac(3)IV, aac3-VI, aac3-VI_1, aac3-VI_2, B9T59_28975, BEN53_26235, BJJ90_27515, BK373_27930, BK400_28640, BVL39_27730, BZL31_07055, BZL69_29365, C5P01_27985, C7235_00480, CR538_26885, CWS33_26775, CXB56_01995, pEC012_00026, SAMEA3472055_02859, SAMEA3472064_03809, SAMEA3472078_05206, SAMEA3472108_05065, SAMEA3472117_05674, SAMEA3472119_04576, SAMEA3472121_05663, SAMEA3485115_04534, SAMEA3751392_04817, SAMEA3752267_04833, SAMEA3752372_05888, SAMEA3752378_04585, SAMEA3752509_02517, SAMEA3752558_04925, SAMEA3752685_02521, SAMEA3753064_04967, SAMEA3753137_02787, SAMEA3753290_03667, SAMEA3753300_05389, SAMEA3753355_02348
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q306W4 (4-261)
      • expression tag (0-3)
      • engineered mutation (157)
  • Heterogens: LLL, ZN, EPE, GOL, EDO, PE3, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6mn5A (A:)
gqqmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpgg
tlvmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiis
dplplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdg
klvrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdp
liflhppeagceecdaarqsig

    Sequence, based on observed residues (ATOM records):
    >6mn5A (A:)
qmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpggtl
vmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiisdp
lplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdgkl
vrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdpli
flhppeagceecdaarqsi

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6mn5B (B:)
gqqmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpgg
tlvmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiis
dplplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdg
klvrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdp
liflhppeagceecdaarqsig

    Sequence, based on observed residues (ATOM records):
    >6mn5B (B:)
qmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpggtl
vmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiisdp
lplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdgkl
vrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdpli
flhppeagceecdaarqsi

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6mn5C (C:)
gqqmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpgg
tlvmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiis
dplplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdg
klvrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdp
liflhppeagceecdaarqsig

    Sequence, based on observed residues (ATOM records):
    >6mn5C (C:)
qmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpggtl
vmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiisdp
lplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdgkl
vrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdpli
flhppeagceecdaarqsi

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6mn5D (D:)
gqqmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpgg
tlvmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiis
dplplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdg
klvrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdp
liflhppeagceecdaarqsig

    Sequence, based on observed residues (ATOM records):
    >6mn5D (D:)
qmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpggtl
vmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiisdp
lplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdgkl
vrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdpli
flhppeagceecdaarqsig

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6mn5E (E:)
gqqmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpgg
tlvmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiis
dplplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdg
klvrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdp
liflhppeagceecdaarqsig

    Sequence, based on observed residues (ATOM records):
    >6mn5E (E:)
qmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpggtl
vmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiisdp
lplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdgkl
vrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdpli
flhppeagceecdaarqsi

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6mn5F (F:)
gqqmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpgg
tlvmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiis
dplplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdg
klvrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdp
liflhppeagceecdaarqsig

    Sequence, based on observed residues (ATOM records):
    >6mn5F (F:)
qmmqyewrkaeligqllnlgvtpggvllvhssfrsvrpledgplgliealraalgpggtl
vmpswsglddepfdpatspvtpdlgvvsdtfwrlpnvkrsahpfafaaagpqaeqiisdp
lplpphspaspvarvheldgqvlllgvghdanttlalaelmakvpygvprhctilqdgkl
vrvdylendhccerfaladrwlkekslqkegpvghafarlirsrdivatalgqlgrdpli
flhppeagceecdaarqsi