PDB entry 6mdn

View 6mdn on RCSB PDB site
Description: the 20s supercomplex engaging the snap-25 n-terminus (class 2)
Deposited on 2018-09-04, released 2018-09-19
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 4.4 Å
R-factor: N/A
AEROSPACI score: 0.04 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Vesicle-fusing ATPase
    Species: Cricetulus griseus [TaxId:10029]
    Gene: NSF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P18708 (Start-746)
      • conflict (481)
      • expression tag (747-761)
  • Chain 'B':
    Compound: Vesicle-fusing ATPase
    Species: Cricetulus griseus [TaxId:10029]
    Gene: NSF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P18708 (24-746)
      • conflict (481)
      • expression tag (747-761)
  • Chain 'C':
    Compound: Vesicle-fusing ATPase
    Species: Cricetulus griseus [TaxId:10029]
    Gene: NSF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P18708 (24-746)
      • expression tag (747-761)
  • Chain 'D':
    Compound: Vesicle-fusing ATPase
    Species: Cricetulus griseus [TaxId:10029]
    Gene: NSF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P18708 (24-746)
      • conflict (481)
      • expression tag (747-761)
  • Chain 'E':
    Compound: Vesicle-fusing ATPase
    Species: Cricetulus griseus [TaxId:10029]
    Gene: NSF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P18708 (24-746)
      • conflict (481)
      • expression tag (747-761)
  • Chain 'F':
    Compound: Vesicle-fusing ATPase
    Species: Cricetulus griseus [TaxId:10029]
    Gene: NSF
    Database cross-references and differences (RAF-indexed):
    • Uniprot P18708 (Start-746)
      • expression tag (747-761)
  • Chain 'H':
    Compound: synaptosomal-associated protein 25
    Species: Rattus norvegicus [TaxId:10116]
    Gene: SNAP25, SNAP
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: syntaxin-1a
    Species: Rattus norvegicus [TaxId:10116]
    Gene: Stx1a, Sap
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: vesicle-associated membrane protein 2
    Species: Rattus norvegicus [TaxId:10116]
    Gene: Vamp2, Syb2
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63045 (Start-99)
      • expression tag (100-116)
  • Chain 'K':
    Compound: Alpha-soluble NSF attachment protein
    Species: Rattus norvegicus [TaxId:10116]
    Gene: Napa, Snap, Snapa
    Database cross-references and differences (RAF-indexed):
    • Uniprot P54921 (Start-295)
      • expression tag (296-310)
  • Chain 'L':
    Compound: Alpha-soluble NSF attachment protein
    Species: Rattus norvegicus [TaxId:10116]
    Gene: Napa, Snap, Snapa
    Database cross-references and differences (RAF-indexed):
    • Uniprot P54921 (Start-295)
      • expression tag (296-310)
  • Heterogens: ATP, ADP

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6mdnA (A:)
mghhhhhhdydipttenlyfqgahmagrsmqaarcptdelslsncavvsekdyqsgqhvi
vrtspnhkyiftlrthpsvvpgsvafslpqrkwaglsigqeievalysfdkakqcigtmt
ieidflqkknidsnpydtdkmaaefiqqfnnqafsvgqqlvfsfndklfgllvkdieamd
psilkgepasgkrqkievglvvgnsqvafekaensslnligkaktkenrqsiinpdwnfe
kmgiggldkefsdifrrafasrvfppeiveqmgckhvkgillygppgcgktllarqigkm
lnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgansglhiiifdeidaickq
rgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdlideallrpgrlevkmeig
lpdekgrlqilhihtarmrghqllsadvdikelavetknfsgaeleglvraaqstamnrh
iiastkvevdmekaeslqvtrgdflaslendikpafgtnqedyasyimngiikwgdpvtr
vlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigf
setakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappq
grklliigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaq
qvkgkkvwigikkllmliemslqmdpeyrvrkflallreegaspldfd

    Sequence, based on observed residues (ATOM records):
    >6mdnA (A:)
pdwnfekmgiggldkefsdifrrafasrvfppeiveqmgckhvkgillygppgcgktlla
rqigkmlnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgansglhiiifdei
daickqrgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdlideallrpgrle
vkmeiglpdekgrlqilhihtarmrghqllsadvdikelavetknfsgaeleglvraaqs
tamnrhiiargdflaslendikpafgtnqedyasyimngiikwgdpvtrvlddgellvqq
tknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigfsetakcqamkk
ifddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappqgrklliigtts
rkdvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaqqvkgkkvwigi
kkllmliemslqmdpeyrvrkflallreega

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6mdnB (B:)
mghhhhhhdydipttenlyfqgahmagrsmqaarcptdelslsncavvsekdyqsgqhvi
vrtspnhkyiftlrthpsvvpgsvafslpqrkwaglsigqeievalysfdkakqcigtmt
ieidflqkknidsnpydtdkmaaefiqqfnnqafsvgqqlvfsfndklfgllvkdieamd
psilkgepasgkrqkievglvvgnsqvafekaensslnligkaktkenrqsiinpdwnfe
kmgiggldkefsdifrrafasrvfppeiveqmgckhvkgillygppgcgktllarqigkm
lnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgansglhiiifdeidaickq
rgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdlideallrpgrlevkmeig
lpdekgrlqilhihtarmrghqllsadvdikelavetknfsgaeleglvraaqstamnrh
iiastkvevdmekaeslqvtrgdflaslendikpafgtnqedyasyimngiikwgdpvtr
vlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigf
setakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappq
grklliigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaq
qvkgkkvwigikkllmliemslqmdpeyrvrkflallreegaspldfd

    Sequence, based on observed residues (ATOM records):
    >6mdnB (B:)
magrsmqaarcptdelslsncavvsekdyqsgqhvivrtspnhkyiftlrthpsvvpgsv
afslpqrkwaglsigqeievalysfdkakqcigtmtieidflqkknidsnpydtdkmaae
fiqqfnnqafsvgqqlvfsfndklfgllvkdieamrqkievglvvgnsqvafekaenssl
nligkaktqsiinpdwnfekmgiggldkefsdifrrafasrvfppeiveqmgckhvkgil
lygppgcgktllarqigkmlnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlg
ansglhiiifdeidaickqrgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpd
lideallrpgrlevkmeiglpdekgrlqilhihtarmrghqllsadvdikelavetknfs
gaeleglvraaqstamnrhiiasvtrgdflaslendikpafgtnqedyasyimngiikwg
dpvtrvlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspd
kmigfsetakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvllk
kappqgrklliigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdker
ttiaqqvkgkkvwigikkllmliemslqmdpeyrvrkflallreega

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6mdnC (C:)
mghhhhhhdydipttenlyfqgahmagrsmqaarcptdelslsncavvsekdyqsgqhvi
vrtspnhkyiftlrthpsvvpgsvafslpqrkwaglsigqeievalysfdkakqcigtmt
ieidflqkknidsnpydtdkmaaefiqqfnnqafsvgqqlvfsfndklfgllvkdieamd
psilkgepasgkrqkievglvvgnsqvafekaensslnligkaktkenrqsiinpdwnfe
kmgiggldkefsdifrrafasrvfppeiveqmgckhvkgillygppgcgktllarqigkm
lnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgansglhiiifdeidaickq
rgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdlideallrpgrlevkmeig
lpdekgrlqilhihtarmrghqllsadvdikelavetknfsgaeleglvraaqstamnrh
iiastkvevdmekaeslqvtrgdflaslendikpafgtnqedyasyimngiikwgdpvtr
vlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigf
setakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappq
grklliigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaq
qvkgkkvwigikkllmliemslqmdpeyrvrkflallreegaspldfd

    Sequence, based on observed residues (ATOM records):
    >6mdnC (C:)
magrsmqaarcptdelslsncavvsekdyqsgqhvivrtspnhkyiftlrthpsvvpgsv
afslpqrkwaglsigqeievalysfdkakqcigtmtieidflqkknidsnpydtdkmaae
fiqqfnnqafsvgqqlvfsfndklfgllvkdieamrqkievglvvgnsqvafekaenssl
nligkaktiinpdwnfekmgiggldkefsdifrrafasrvfppeiveqmgckhvkgilly
gppgcgktllarqigkmlnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgan
sglhiiifdeidaickqrgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdli
deallrpgrlevkmeiglpdekgrlqilhihtarmrghqllsadvdikelavetknfsga
eleglvraaqstamnrhiekaeslqvtrgdflaslendikpafgtnqedyasyimngiik
wgdpvtrvlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikics
pdkmigfsetakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvl
lkkappqgrklliigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdk
erttiaqqvkgkkvwigikkllmliemslqmdpeyrvrkflallreega

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6mdnD (D:)
mghhhhhhdydipttenlyfqgahmagrsmqaarcptdelslsncavvsekdyqsgqhvi
vrtspnhkyiftlrthpsvvpgsvafslpqrkwaglsigqeievalysfdkakqcigtmt
ieidflqkknidsnpydtdkmaaefiqqfnnqafsvgqqlvfsfndklfgllvkdieamd
psilkgepasgkrqkievglvvgnsqvafekaensslnligkaktkenrqsiinpdwnfe
kmgiggldkefsdifrrafasrvfppeiveqmgckhvkgillygppgcgktllarqigkm
lnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgansglhiiifdeidaickq
rgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdlideallrpgrlevkmeig
lpdekgrlqilhihtarmrghqllsadvdikelavetknfsgaeleglvraaqstamnrh
iiastkvevdmekaeslqvtrgdflaslendikpafgtnqedyasyimngiikwgdpvtr
vlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigf
setakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappq
grklliigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaq
qvkgkkvwigikkllmliemslqmdpeyrvrkflallreegaspldfd

    Sequence, based on observed residues (ATOM records):
    >6mdnD (D:)
magrsmqaarcptdelslsncavvsekdyqsgqhvivrtspnhkyiftlrthpsvvpgsv
afslpqrkwaglsigqeievalysfdkakqcigtmtieidflqkknidsnpydtdkmaae
fiqqfnnqafsvgqqlvfsfndklfgllvkdieamrqkievglvvgnsqvafekaenssl
nligkaktiinpdwnfekmgiggldkefsdifrrafasrvfppeiveqmgckhvkgilly
gppgcgktllarqigkmlnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgan
sglhiiifdeidaickqrgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdli
deallrpgrlevkmeiglpdekgrlqilhihtarmrghqllsadvdikelavetknfsga
eleglvraaqstamnrhiivdmekaeslqvtrgdflaslendikpafgtnqedyasyimn
giikwgdpvtrvlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfi
kicspdkmigfsetakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqa
llvllkkappqgrklliigttsrkdvlqememlnafsttihvpniatgeqllealellgn
fkdkerttiaqqvkgkkvwigikkllmliemslqmdpeyrvrkflallreega

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6mdnE (E:)
mghhhhhhdydipttenlyfqgahmagrsmqaarcptdelslsncavvsekdyqsgqhvi
vrtspnhkyiftlrthpsvvpgsvafslpqrkwaglsigqeievalysfdkakqcigtmt
ieidflqkknidsnpydtdkmaaefiqqfnnqafsvgqqlvfsfndklfgllvkdieamd
psilkgepasgkrqkievglvvgnsqvafekaensslnligkaktkenrqsiinpdwnfe
kmgiggldkefsdifrrafasrvfppeiveqmgckhvkgillygppgcgktllarqigkm
lnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgansglhiiifdeidaickq
rgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdlideallrpgrlevkmeig
lpdekgrlqilhihtarmrghqllsadvdikelavetknfsgaeleglvraaqstamnrh
iiastkvevdmekaeslqvtrgdflaslendikpafgtnqedyasyimngiikwgdpvtr
vlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigf
setakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappq
grklliigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaq
qvkgkkvwigikkllmliemslqmdpeyrvrkflallreegaspldfd

    Sequence, based on observed residues (ATOM records):
    >6mdnE (E:)
magrsmqaarcptdelslsncavvsekdyqsgqhvivrtspnhkyiftlrthpsvvpgsv
afslpqrkwaglsigqeievalysfdkakqcigtmtieidflqkknidsnpydtdkmaae
fiqqfnnqafsvgqqlvfsfndklfgllvkdieamrqkievglvvgnsqvafekaenssl
nligkaktfekmgiggldkefsdifrrafasrvfckhvkgillygppgcgktllarqigk
mlnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgansglhiiifdeidaick
qrgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdlideallrpgrlevkmei
glpdekgrlqilhihtarmrghqllsadvdikelavetknfsgaeleglvraaqstamnr
hiivdmekaeslqvtrgdflaslendikpafgtnqedyasyimngiikwgdpvtrvlddg
ellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigfsetak
cqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappqgrkll
iigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaqqvkgk
kvwigikkllmliemslqmdpeyrvrkflallreega

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6mdnF (F:)
mghhhhhhdydipttenlyfqgahmagrsmqaarcptdelslsncavvsekdyqsgqhvi
vrtspnhkyiftlrthpsvvpgsvafslpqrkwaglsigqeievalysfdkakqcigtmt
ieidflqkknidsnpydtdkmaaefiqqfnnqafsvgqqlvfsfndklfgllvkdieamd
psilkgepasgkrqkievglvvgnsqvafekaensslnligkaktkenrqsiinpdwnfe
kmgiggldkefsdifrrafasrvfppeiveqmgckhvkgillygppgcgktllarqigkm
lnarepkvvngpeilnkyvgeseanirklfadaeeeqrrlgansglhiiifdeidaickq
rgsmagstgvhdtvvnqllskidgveqlnnilvigmtnrpdlideallrpgrlevkmeig
lpdekgrlqilhihtarmrghqllsadvdikelavetknfsgaeleglvraaqstamnrh
iiastkvevdmekaeslqvtrgdflaslendikpafgtnqedyasyimngiikwgdpvtr
vlddgellvqqtknsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigf
setakcqamkkifddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappq
grklliigttsrkdvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaq
qvkgkkvwigikkllmliemslqmdpeyrvrkflallreegaspldfd

    Sequence, based on observed residues (ATOM records):
    >6mdnF (F:)
giggldkefsdifrrafasrvkhvkgillygppgcgktllarqigkmlnarepkvvngpe
ilnkyvgeseanirklfadaeeeqrrlgansglhiiifdeihdtvvnqllskidgveqln
nilvigmtpdekgrlqilhihtarmadvdikelavetknfsgaeleglvraaqstamnrh
ieslqvtrgdflaslendikpafgtnqedyasyimngiikwgdpvtrvlddgellvqqtk
nsdrtplvsvllegpphsgktalaakiaeesnfpfikicspdkmigfsetakcqamkkif
ddayksqlscvvvddierlldyvpigprfsnlvlqallvllkkappqgrklliigttsrk
dvlqememlnafsttihvpniatgeqllealellgnfkdkerttiaqqvkgkkvwigikk
llmliemslqmdpeyrvrkflallreega

  • Chain 'H':
    Sequence, based on SEQRES records:
    >6mdnH (H:)
masmaedadmrneleemqrradqladeslestrrmlqlveeskdagirtlvmldeqgeql
drveegmnhinqdmkeaeknlkdlgkccglficpcnklkssdaykkawgnnqdgvvasqp
arvvdereqmaisggfirrvtndarenemdenleqvsgiignlrhmaldmgneidtqnrq
idrimekadsnktrideanqratkmlg

    Sequence, based on observed residues (ATOM records):
    >6mdnH (H:)
maedadmrneleemqrradqladeslestrrmlqlveeskdagirtlvmldeqgeqldrv
eegmnhinqdmkeaeknlkdlgkarenemdenleqvsgiignlrhmaldmgneidtqnrq
idrimekadsnktrideanqratkmlg

  • Chain 'I':
    Sequence, based on SEQRES records:
    >6mdnI (I:)
mkdrtqelrtakdsdddddvtvtvdrdrfmdeffeqveeirgfidkiaenveevkrkhsa
ilaspnpdektkeeleelmsdikktankvrsklksieqsieqeeglnrssadlrirktqh
stlsrkfvevmseynatqsdyrerskgriqrqleitgrtttseeledmlesgnpaifasg
iimdssiskqalseietrhseiiklensirelhdmfmdmamlvesqgemidrieynveha
vdyveravsdtkkavk

    Sequence, based on observed residues (ATOM records):
    >6mdnI (I:)
alseietrhseiiklensirelhdmfmdmamlvesqgemidrieynvehavdyveravsd
tkkavk

  • Chain 'J':
    Sequence, based on SEQRES records:
    >6mdnJ (J:)
masyyhhhhhhdydiptsenlyfqgashmsataatvppaapageggppapppnltsnrrl
qqtqaqvdevvdimrvnvdkvlerdqklselddradalqagasqfetsaaklkrkyw

    Sequence, based on observed residues (ATOM records):
    >6mdnJ (J:)
nrrlqqtqaqvdevvdimrvnvdkvlerdqklselddradalqagasqfetsaaklkrky
w

  • Chain 'K':
    Sequence, based on SEQRES records:
    >6mdnK (K:)
mhhhhhhhhhhenlyfqgmdtsgkqaeamallaeaerkvknsqsffsglfggsskieeac
eiyaraanmfkmaknwsaagnafcqaaqlhlqlqskhdaatcfvdagnafkkadpqeain
clmraieiytdmgrftiaakhhisiaeiyetelvdvekaiahyeqsadyykgeesnssan
kcllkvagyaaqleqyqkaidiyeqvgtsamdspllkysakdyffkaalchfcidmlnak
lavqkyeelfpafsdsrecklmkklleaheeqnvdsytesvkeydsisrldqwlttmllr
ikktiqgdeedlr

    Sequence, based on observed residues (ATOM records):
    >6mdnK (K:)
aeamallaeaerkvknsqsffsglfggsskieeaceiyaraanmfkmaknwsaagnafcq
aaqlhlqlqskhdaatcfvdagnafkkadpqeainclmraieiytdmgrftiaakhhisi
aeiyetelvdvekaiahyeqsadyykgeesnssankcllkvagyaaqleqyqkaidiyeq
vgtsamdspllkysakdyffkaalchfcidmlnaklavqkyeelfpafsdsrecklmkkl
leaheeqnvdsytesvkeydsisrldqwlttmllrikktiqgdeed

  • Chain 'L':
    Sequence, based on SEQRES records:
    >6mdnL (L:)
mhhhhhhhhhhenlyfqgmdtsgkqaeamallaeaerkvknsqsffsglfggsskieeac
eiyaraanmfkmaknwsaagnafcqaaqlhlqlqskhdaatcfvdagnafkkadpqeain
clmraieiytdmgrftiaakhhisiaeiyetelvdvekaiahyeqsadyykgeesnssan
kcllkvagyaaqleqyqkaidiyeqvgtsamdspllkysakdyffkaalchfcidmlnak
lavqkyeelfpafsdsrecklmkklleaheeqnvdsytesvkeydsisrldqwlttmllr
ikktiqgdeedlr

    Sequence, based on observed residues (ATOM records):
    >6mdnL (L:)
aeamallaeaerkvknsqsffsglfggsskieeaceiyaraanmfkmaknwsaagnafcq
aaqlhlqlqskhdaatcfvdagnafkkadpqeainclmraieiytdmgrftiaakhhisi
aeiyetelvdvekaiahyeqsadyykgeesnssankcllkvagyaaqleqyqkaidiyeq
vgtsamdspllkysakdyffkaalchfcidmlnaklavqkyeelfpafsdsrecklmkkl
leaheeqnvdsytesvkeydsisrldqwlttmllrikktiqgdeed