PDB entry 6m4w

View 6m4w on RCSB PDB site
Description: Crystal structure of MBP fused split FKBP-FRB T2098L mutant in complex with rapamycin
Class: isomerase
Keywords: Rapamycin, complex, kinase, isomerase
Deposited on 2020-03-09, released 2020-08-26
The last revision prior to the SCOPe 2.07 freeze date was dated 2020-09-16, with a file datestamp of 2020-09-11.
Experiment type: XRAY
Resolution: 3.11 Å
R-factor: N/A
AEROSPACI score: 0.2 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: chimera of Maltose/maltodextrin-binding periplasmic protein and Peptidyl-prolyl cis-trans isomerase FKBP1A
    Species: Homo sapiens [TaxId:9606]
    Gene: FKBP1A, FKBP1, FKBP12
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEX9 (3-368)
      • linker (2)
      • engineered mutation (84-85)
      • engineered mutation (174-175)
      • engineered mutation (241)
      • linker (369-372)
    • Uniprot P62942 (373-404)
    Domains in SCOPe 2.07: d6m4wa_
  • Chain 'B':
    Compound: chimera of Maltose/maltodextrin-binding periplasmic protein and Peptidyl-prolyl cis-trans isomerase FKBP1A
    Species: Homo sapiens [TaxId:9606]
    Gene: FKBP1A, FKBP1, FKBP12
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEX9 (Start-368)
      • engineered mutation (84-85)
      • engineered mutation (241)
      • linker (369-372)
    • Uniprot P62942 (373-404)
    Domains in SCOPe 2.07: d6m4wb_
  • Chain 'C':
    Compound: chimera of Maltose/maltodextrin-binding periplasmic protein and Peptidyl-prolyl cis-trans isomerase FKBP1A
    Species: Homo sapiens [TaxId:9606]
    Gene: FKBP1A, FKBP1, FKBP12
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0AEX9 (3-368)
      • engineered mutation (84-85)
      • engineered mutation (174-175)
      • engineered mutation (241)
      • linker (369-372)
    • Uniprot P62942 (373-404)
    Domains in SCOPe 2.07: d6m4wc_
  • Chain 'D':
    Compound: Peptidyl-prolyl cis-trans isomerase FKBP1A
    Species: Homo sapiens [TaxId:9606]
    Gene: FKBP1A, FKBP1, FKBP12
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: Peptidyl-prolyl cis-trans isomerase FKBP1A
    Species: Homo sapiens [TaxId:9606]
    Gene: FKBP1A, FKBP1, FKBP12
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: Peptidyl-prolyl cis-trans isomerase FKBP1A
    Species: Homo sapiens [TaxId:9606]
    Gene: FKBP1A, FKBP1, FKBP12
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Serine/threonine-protein kinase mTOR
    Species: Homo sapiens [TaxId:9606]
    Gene: mTOR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42345 (2-End)
      • expression tag (1)
      • engineered mutation (79)
    Domains in SCOPe 2.07: d6m4wg1, d6m4wg2
  • Chain 'H':
    Compound: Serine/threonine-protein kinase mTOR
    Species: Homo sapiens [TaxId:9606]
    Gene: mTOR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42345 (2-End)
      • expression tag (1)
      • engineered mutation (79)
    Domains in SCOPe 2.07: d6m4wh1, d6m4wh2
  • Chain 'I':
    Compound: Serine/threonine-protein kinase mTOR
    Species: Homo sapiens [TaxId:9606]
    Gene: mTOR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42345 (2-End)
      • expression tag (1)
      • engineered mutation (79)
    Domains in SCOPe 2.07: d6m4wi1, d6m4wi2
  • Heterogens: RAP, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6m4wA (A:)
gsmkieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgp
diifwahdrfggyaqsgllaeitpaaafqdklypftwdavryngkliaypiavealsliy
nkdllpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyaagkyd
ikdvgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidt
savnygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdk
plgavalksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtv
dealkdaqtnaaamgvqvetispgdgrtfpkrgqtcvvhytgmle

    Sequence, based on observed residues (ATOM records): (download) 
    >6m4wA (A:)
mkieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdi
ifwahdrfggyaqsgllaeitpaaafqdklypftwdavryngkliaypiavealsliynk
dllpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyaagkydik
dvgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtsa
vnygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkpl
gavalksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvde
alkdaqtnaaamgvqvetispgdgrtfpkrgqtcvvhytgmle

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6m4wB (B:)
gsmkieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgp
diifwahdrfggyaqsgllaeitpaaafqdklypftwdavryngkliaypiavealsliy
nkdllpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyaagkyd
ikdvgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidt
savnygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdk
plgavalksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtv
dealkdaqtnaaamgvqvetispgdgrtfpkrgqtcvvhytgmle

    Sequence, based on observed residues (ATOM records): (download) 
    >6m4wB (B:)
ieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdiif
wahdrfggyaqsgllaeitpaaafqdklypftwdavryngkliaypiavealsliynkdl
lpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkygkydikdvgv
dnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtsavnyg
vtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplgava
lksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdealkd
aqtnaaamgvqvetispgdgrtfpkrgqtcvvhytgmle

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6m4wC (C:)
gsmkieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgp
diifwahdrfggyaqsgllaeitpaaafqdklypftwdavryngkliaypiavealsliy
nkdllpnppktweeipaldkelkakgksalmfnlqepyftwpliaadggyafkyaagkyd
ikdvgvdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidt
savnygvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdk
plgavalksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtv
dealkdaqtnaaamgvqvetispgdgrtfpkrgqtcvvhytgmle

    Sequence, based on observed residues (ATOM records): (download) 
    >6m4wC (C:)
kieegklviwingdkgynglaevgkkfekdtgikvtvehpdkleekfpqvaatgdgpdii
fwahdrfggyaqsgllaeitpaaafqdklypftwdavryngkliaypiavealsliynkd
llpnppktweeipaldkelkaksalmfnlqepyftwpliaadggyafkyaagkydikdvg
vdnagakagltflvdliknkhmnadtdysiaeaafnkgetamtingpwawsnidtsavny
gvtvlptfkgqpskpfvgvlsaginaaspnkelakeflenylltdegleavnkdkplgav
alksyeeelakdpriaatmenaqkgeimpnipqmsafwyavrtavinaasgrqtvdealk
daqtnaaamgvqvetispgdgrfpkrgqtcvvhytgmle

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >6m4wG (G:)
gsilwhemwhegleeasrlyfgernvkgmfevleplhammergpqtlketsfnqaygrdl
meaqewcrkymksgnvkdllqawdlyyhvfrrisk

    Sequence, based on observed residues (ATOM records): (download) 
    >6m4wG (G:)
silwhemwhegleeasrlyfgernvkgmfevleplhammergpqtlketsfnqaygrdlm
eaqewcrkymksgnvkdllqawdlyyhvfrris

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >6m4wH (H:)
gsilwhemwhegleeasrlyfgernvkgmfevleplhammergpqtlketsfnqaygrdl
meaqewcrkymksgnvkdllqawdlyyhvfrrisk

    Sequence, based on observed residues (ATOM records): (download) 
    >6m4wH (H:)
silwhemwhegleeasrlyfgernvkgmfevleplhammergpqtlketsfnqaygrdlm
eaqewcrkymksgnvkdllqawdlyyhvfrris

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >6m4wI (I:)
gsilwhemwhegleeasrlyfgernvkgmfevleplhammergpqtlketsfnqaygrdl
meaqewcrkymksgnvkdllqawdlyyhvfrrisk

    Sequence, based on observed residues (ATOM records): (download) 
    >6m4wI (I:)
silwhemwhegleeasrlyfgernvkgmfevleplhammergpqtlketsfnqaygrdlm
eaqewcrkymksgnvkdllqawdlyyhvfrris