PDB entry 6m4l

View 6m4l on RCSB PDB site
Description: X-ray crystal structure of the E249Q mutant of alpha-amylase I from Eisenia fetida
Class: hydrolase
Keywords: TIM barrel, GH family 13, carbohydrase, cold-active, HYDROLASE
Deposited on 2020-03-07, released 2020-09-16
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-09-16, with a file datestamp of 2020-09-11.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: N/A
AEROSPACI score: 0.52 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: alpha-amylase
    Species: Eisenia fetida [TaxId:6396]
    Gene: alpha-amylase
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A173N065 (4-496)
      • expression tag (3)
      • engineered mutation (235)
      • expression tag (497-498)
    Domains in SCOPe 2.08: d6m4la1, d6m4la2, d6m4la3, d6m4la4
  • Heterogens: CA, CL, SO4, ACT, TRS, PG4, EDO, PGE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6m4lA (A:)
eaefqyfgsyycqpgrdvivhlfewkwtdierecqwladhnycgvqvsppnehrivtdpp
ypwwqryqpvsytmnsrsgsetqfrnmvttcnnlgvyiyvdgvlnhmtgggsgtgsdgns
fdgdslqypgvpygpndfhgsddcstsdgeihdynnptevrncrlsglrdldgsanyvrd
veaefanrligwgvagfrldackhmwpgdleailgrlnqlntqwfpagrnayiyqqvidl
ggeavkateytylgrvtefkhgqnlgnvvrknngqrlanlvnfgegwgqlsgfdalvfid
nhdnqrghgaggfgtiltffeanmykiatafelawdyghvrlmssynwprnivngvdtnd
wvgpptngggdtkdvecfngewicehrwremynmvrfhnvaignpvsnwwdngfqaiafg
rgnrgfifinnedfaitqtlqtglpggeycdviscdnnrppcgnsggacratvivngdgt
atfdvpngenpmiaihvfleqkliseedlnsavdhhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >6m4lA (A:)
fqyfgsyycqpgrdvivhlfewkwtdierecqwladhnycgvqvsppnehrivtdppypw
wqryqpvsytmnsrsgsetqfrnmvttcnnlgvyiyvdgvlnhmtgggsgtgsdgnsfdg
dslqypgvpygpndfhgsddcstsdgeihdynnptevrncrlsglrdldgsanyvrdvea
efanrligwgvagfrldackhmwpgdleailgrlnqlntqwfpagrnayiyqqvidlgge
avkateytylgrvtefkhgqnlgnvvrknngqrlanlvnfgegwgqlsgfdalvfidnhd
nqrghgaggfgtiltffeanmykiatafelawdyghvrlmssynwprnivngvdtndwvg
pptngggdtkdvecfngewicehrwremynmvrfhnvaignpvsnwwdngfqaiafgrgn
rgfifinnedfaitqtlqtglpggeycdviscdnnrppcgnsggacratvivngdgtatf
dvpngenpmiaihvfl