PDB entry 6lml

View 6lml on RCSB PDB site
Description: cryo-em structure of the human glucagon receptor in complex with gi1
Deposited on 2019-12-26, released 2020-04-01
The last revision was dated 2020-04-01, with a file datestamp of 2020-03-27.
Experiment type: EM
Resolution: 3.9 Å
R-factor: N/A
AEROSPACI score: 0.04 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Guanine nucleotide-binding protein G(i) subunit alpha-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNAI1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P63096 (Start-353)
      • engineered mutation (46)
      • engineered mutation (202)
      • engineered mutation (244)
      • engineered mutation (325)
  • Chain 'B':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Species: Homo sapiens [TaxId:9606]
    Gene: GNB1
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
    Species: Homo sapiens [TaxId:9606]
    Gene: GNG2
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: scFv16
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6LML (Start-246)
  • Chain 'E':
    Compound: glucagon
    Species: HOMO SAPIENS, synthetic [TaxId:9606]
    Database cross-references and differences (RAF-indexed):
  • Chain 'R':
    Compound: Glucagon receptor
    Species: Homo sapiens [TaxId:9606]
    Gene: GCGR
    Database cross-references and differences (RAF-indexed):
    • Uniprot P47871 (0-End)
      • engineered mutation (99)
      • engineered mutation (173)
      • engineered mutation (339)

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6lmlA (A:)
mgctlsaedkaaverskmidrnlredgekaarevkllllgagesgkntivkqmkiiheag
yseeeckqykavvysntiqsiiaiiramgrlkidfgdsaraddarqlfvlagaaeegfmt
aelagvikrlwkdsgvqacfnrsreyqlndsaayylndldriaqpnyiptqqdvlrtrvk
ttgivethftfkdlhfkmfdvgaqrserkkwihcfegvtaiifcvalsdydlvlaedeem
nrmhasmklfdsicnnkwftdtsiilflnkkdlfeekikksplticypeyagsntyeeaa
ayiqcqfedlnkrkdtkeiythftcstdtknvqfvfdavtdviiknnlkdcglf

    Sequence, based on observed residues (ATOM records):
    >6lmlA (A:)
lsaedkaaverskmidrnlredgekaarevkllllgagesgkntivkqmkitgivethft
fkdlhfkmfdvgaqrserkkwihcfegvtaiifcvalsdydlvlaedeemnrmhasmklf
dsicnnkwftdtsiilflnkkdlfeekikksplticypeyagsntyeeaaayiqcqfedl
nkrkdtkeiythftcstdtknvqfvfdavtdviiknnlkdcglf

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6lmlB (B:)
mhhhhhhgsllqseldqlrqeaeqlknqirdarkacadatlsqitnnidpvgriqmrtrr
tlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaiplrsswvmtcayapsg
nyvacggldnicsiynlktregnvrvsrelaghtgylsccrflddnqivtssgdttcalw
dietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvregmcrqtftghesdi
naicffpngnafatgsddatcrlfdlradqelmtyshdniicgitsvsfsksgrlllagy
ddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswdsflkiwn

    Sequence, based on observed residues (ATOM records):
    >6lmlB (B:)
nnidpvgriqmrtrrtlrghlakiyamhwgtdsrllvsasqdgkliiwdsyttnkvhaip
lrsswvmtcayapsgnyvacggldnicsiynlktregnvrvsrelaghtgylsccrfldd
nqivtssgdttcalwdietgqqtttftghtgdvmslslapdtrlfvsgacdasaklwdvr
egmcrqtftghesdinaicffpngnafatgsddatcrlfdlradqelmtyshdniicgit
svsfsksgrlllagyddfncnvwdalkadragvlaghdnrvsclgvtddgmavatgswds
flkiwn

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6lmlC (C:)
masnntasiaqarklveqlkmeanidrikvskaaadlmayceahakedplltpvpasenp
frekkffcail

    Sequence, based on observed residues (ATOM records):
    >6lmlC (C:)
kvskaaadlmayceahakedplltpvpasenpf

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6lmlD (D:)
dvqlvesggglvqpggsrklscsasgfafssfgmhwvrqapekglewvayissgsgtiyy
adtvkgrftisrddpkntlflqmtslrsedtamyycvrsiyyygsspfdfwgqgttltvs
sggggsggggsggggsdivmtqatssvpvtpgesvsiscrssksllhsngntylywflqr
pgqspqlliyrmsnlasgvpdrfsgsgsgtaftltisrleaedvgvyycmqhleypltfg
agtklel

    Sequence, based on observed residues (ATOM records):
    >6lmlD (D:)
vqlvesggglvqpggsrklscsasgfafssfgmhwvrqapekglewvayissgsgtiyya
dtvkgrftisrddpkntlflqmtslrsedtamyycvrsiyyygsspfdfwgqgttltvss
sdivmtqatssvpvtpgesvsiscrssksllhsngntylywflqrpgqspqlliyrmsnl
asgvpdrfsgsgsgtaftltisrleaedvgvyycmqhleypltfgagtklel

  • Chain 'E':
    Sequence; same for both SEQRES and ATOM records:
    >6lmlE (E:)
hsqgtftsdyskyldsrraqdfvqwlmnt

  • Chain 'R':
    Sequence, based on SEQRES records:
    >6lmlR (R:)
qvmdflfekwklygdqchhnlsllppptelvcnrtfdkyscwpdtpanttaniscpwylp
whhkvqhrfvfkrcgpdgqwvrgprgqpwrdasqcqmdgreievqkevakmyssfqvmyt
vgyslslgalllalailgglsklhctrnaihanlfasfvlkassvlvidgllrwrysqki
gddlsvstwlsdgavagcrvaavfmqygivanycwllveglylhnllglatlpersffsl
ylgigwgapmlfvvpwavvkclfenvqcwtsndnmgfwwilrfpvflailinffifvriv
qllvaklrarqmhhtdykfrlakstltlipllgvhevvfmfvtdehaqgtlrsaklffdl
flssfqgllvavlycflnkevqselrrrwhrwrlgkvlweerntsngsgsedqvdprlid
gk

    Sequence, based on observed residues (ATOM records):
    >6lmlR (R:)
qvmdflfekwklygdqchhnlsllppptelvcnrtfdkyscwpdtpanttaniscpwylp
whhkvqhrfvfkrcgpdgqwvrgprgqpwrdasqcqmdgreievqkevakmyssfqvmyt
vgyslslgalllalailgglsklhctrnaihanlfasfvlkassvlvidgllrwrysqki
gddlsvstwlsdgavagcrvaavfmqygivanycwllveglylhnllglatlpersffsl
ylgigwgapmlfvvpwavvkclfenvqcwtsndnmgfwwilrfpvflailinffifvriv
qllvaklrarqmhhtdykfrlakstltlipllgvhevvfmfvtdehaqgtlrsaklffdl
flssfqgllvavlycflnkevqselrrrwhrwrlg