PDB entry 6kuz

View 6kuz on RCSB PDB site
Description: E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSL01
Class: hydrolase
Keywords: beta-galactosidase, fluorescent probe, HYDROLASE
Deposited on 2019-09-03, released 2020-07-08
The last revision prior to the SCOPe 2.07 freeze date was dated 2020-07-08, with a file datestamp of 2020-07-03.
Experiment type: XRAY
Resolution: 2.83 Å
R-factor: N/A
AEROSPACI score: 0.15 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: beta-galactosidase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: lacZ, b0344, JW0335
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00722 (Start-1023)
      • engineered mutation (537)
    Domains in SCOPe 2.07: d6kuza1, d6kuza2, d6kuza3, d6kuza4, d6kuza5
  • Chain 'B':
    Compound: beta-galactosidase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: lacZ, b0344, JW0335
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00722 (Start-1023)
      • engineered mutation (537)
    Domains in SCOPe 2.07: d6kuzb1, d6kuzb2, d6kuzb3, d6kuzb4, d6kuzb5
  • Chain 'C':
    Compound: beta-galactosidase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: lacZ, b0344, JW0335
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00722 (Start-1023)
      • engineered mutation (537)
    Domains in SCOPe 2.07: d6kuzc1, d6kuzc2, d6kuzc3, d6kuzc4, d6kuzc5
  • Chain 'D':
    Compound: beta-galactosidase
    Species: Escherichia coli K-12 [TaxId:83333]
    Gene: lacZ, b0344, JW0335
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00722
      • engineered mutation (537)
    Domains in SCOPe 2.07: d6kuzd1, d6kuzd2, d6kuzd3, d6kuzd4, d6kuzd5
  • Heterogens: DVL, NA, DMS, MG, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6kuzA (A:)
mtmitdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewr
fawfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenp
tgcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflra
genrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsrav
leaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpk
lwsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrheh
hplhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethg
mvpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvd
psrpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqya
hamgnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndr
qfcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvald
gkplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqq
wrlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltp
lrdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlitta
hawqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlg
lgpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfni
srysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlv
wcqk

    Sequence, based on observed residues (ATOM records): (download) 
    >6kuzA (A:)
itdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfaw
fpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgc
ysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragen
rlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravlea
evqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklws
aeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhpl
hgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvp
mnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsr
pvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqyaham
gnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfc
mnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkp
lasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrl
aenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrd
qftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittahaw
qhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgp
qenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisry
sqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcq
k

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6kuzB (B:)
mtmitdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewr
fawfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenp
tgcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflra
genrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsrav
leaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpk
lwsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrheh
hplhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethg
mvpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvd
psrpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqya
hamgnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndr
qfcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvald
gkplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqq
wrlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltp
lrdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlitta
hawqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlg
lgpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfni
srysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlv
wcqk

    Sequence, based on observed residues (ATOM records): (download) 
    >6kuzB (B:)
itdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfaw
fpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgc
ysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragen
rlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravlea
evqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklws
aeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhpl
hgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvp
mnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsr
pvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqyaham
gnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfc
mnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkp
lasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrl
aenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrd
qftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittahaw
qhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgp
qenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisry
sqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcq
k

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >6kuzC (C:)
mtmitdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewr
fawfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenp
tgcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflra
genrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsrav
leaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpk
lwsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrheh
hplhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethg
mvpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvd
psrpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqya
hamgnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndr
qfcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvald
gkplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqq
wrlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltp
lrdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlitta
hawqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlg
lgpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfni
srysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlv
wcqk

    Sequence, based on observed residues (ATOM records): (download) 
    >6kuzC (C:)
itdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfaw
fpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgc
ysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragen
rlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravlea
evqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklws
aeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhpl
hgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvp
mnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsr
pvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqyaham
gnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfc
mnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkp
lasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrl
aenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrd
qftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittahaw
qhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgp
qenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisry
sqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcq
k

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >6kuzD (D:)
mtmitdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewr
fawfpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenp
tgcysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflra
genrlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsrav
leaevqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpk
lwsaeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrheh
hplhgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethg
mvpmnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvd
psrpvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqya
hamgnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndr
qfcmnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvald
gkplasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqq
wrlaenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltp
lrdqftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlitta
hawqhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlg
lgpqenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfni
srysqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlv
wcqk

    Sequence, based on observed residues (ATOM records): (download) 
    >6kuzD (D:)
itdslavvlqrrdwenpgvtqlnrlaahppfaswrnseeartdrpsqqlrslngewrfaw
fpapeavpeswlecdlpeadtvvvpsnwqmhgydapiytnvtypitvnppfvptenptgc
ysltfnvdeswlqegqtriifdgvnsafhlwcngrwvgygqdsrlpsefdlsaflragen
rlavmvlrwsdgsyledqdmwrmsgifrdvsllhkpttqisdfhvatrfnddfsravlea
evqmcgelrdylrvtvslwqgetqvasgtapfggeiiderggyadrvtlrlnvenpklws
aeipnlyravvelhtadgtlieaeacdvgfrevriengllllngkpllirgvnrhehhpl
hgqvmdeqtmvqdillmkqnnfnavrcshypnhplwytlcdryglyvvdeaniethgmvp
mnrltddprwlpamservtrmvqrdrnhpsviiwslgnesghganhdalyrwiksvdpsr
pvqyegggadttatdiicpmyarvdedqpfpavpkwsikkwlslpgetrplilcqyaham
gnslggfakywqafrqyprlqggfvwdwvdqslikydengnpwsayggdfgdtpndrqfc
mnglvfadrtphpalteakhqqqffqfrlsgqtievtseylfrhsdnellhwmvaldgkp
lasgevpldvapqgkqlielpelpqpesagqlwltvrvvqpnatawseaghisawqqwrl
aenlsvtlpaashaiphlttsemdfcielgnkrwqfnrqsgflsqmwigdkkqlltplrd
qftrapldndigvseatridpnawverwkaaghyqaeaallqctadtladavlittahaw
qhqgktlfisrktyridgsgqmaitvdvevasdtphpariglncqlaqvaervnwlglgp
qenypdrltaacfdrwdlplsdmytpyvfpsenglrcgtrelnygphqwrgdfqfnisry
sqqqlmetshrhllhaeegtwlnidgfhmgiggddswspsvsaefqlsagryhyqlvwcq