PDB entry 6kus

View 6kus on RCSB PDB site
Description: Crystal strcuture of PETase S121E, D186H, S242T, N246D mutant from Ideonella sakaiensis
Class: hydrolase
Keywords: PETase, PET hydrolase, HYDROLASE
Deposited on 2019-09-02, released 2020-09-02
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-09-02, with a file datestamp of 2020-08-28.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: N/A
AEROSPACI score: 0.45 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Poly(ethylene terephthalate) hydrolase
    Species: Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6) [TaxId:1547922]
    Gene: ISF6_4831
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A0K8P6T7 (21-277)
      • expression tag (17-20)
      • engineered mutation (108)
      • engineered mutation (173)
      • engineered mutation (229)
      • engineered mutation (233)
      • expression tag (278)
    Domains in SCOPe 2.08: d6kusa1, d6kusa2, d6kusa3
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6kusA (A:)
mgsshhhhhhssglvprgshmrgpnptaasleasagpftvrsftvsrpsgygagtvyypt
naggtvgaiaivpgytarqssikwwgprlashgfvvitidtnstldqpesrssqqmaalr
qvaslngtssspiygkvdtarmgvmgwsmggggslisaannpslkaaapqapwhsstnfs
svtvptlifacendsiapvnssalpiydsmsrnakqfleinggshscantgnsdqaligk
kgvawmkrfmdndtrystfacenpnstrvsdfrtancsledpaankarkeaelaaataeq

    Sequence, based on observed residues (ATOM records): (download) 
    >6kusA (A:)
gshmrgpnptaasleasagpftvrsftvsrpsgygagtvyyptnaggtvgaiaivpgyta
rqssikwwgprlashgfvvitidtnstldqpesrssqqmaalrqvaslngtssspiygkv
dtarmgvmgwsmggggslisaannpslkaaapqapwhsstnfssvtvptlifacendsia
pvnssalpiydsmsrnakqfleinggshscantgnsdqaligkkgvawmkrfmdndtrys
tfacenpnstrvsdfrtancsl