PDB entry 6kf1

View 6kf1 on RCSB PDB site
Description: Microbial Hormone-sensitive lipase- E53 mutant S162A
Class: hydrolase
Keywords: Esterase, Microbial Hormone-sensitive lipase, hydrolase
Deposited on 2019-07-05, released 2020-07-08
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-08, with a file datestamp of 2020-07-03.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Lipase
    Species: Erythrobacter longus [TaxId:1044]
    Gene: EH31_02760
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A074MDU6 (0-308)
      • engineered mutation (158)
    Domains in SCOPe 2.08: d6kf1a_
  • Chain 'B':
    Compound: Lipase
    Species: Erythrobacter longus [TaxId:1044]
    Gene: EH31_02760
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A074MDU6 (0-308)
      • engineered mutation (158)
    Domains in SCOPe 2.08: d6kf1b_
  • Chain 'C':
    Compound: Lipase
    Species: Erythrobacter longus [TaxId:1044]
    Gene: EH31_02760
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A074MDU6 (0-308)
      • engineered mutation (158)
    Domains in SCOPe 2.08: d6kf1c_
  • Chain 'D':
    Compound: Lipase
    Species: Erythrobacter longus [TaxId:1044]
    Gene: EH31_02760
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A074MDU6 (0-309)
      • engineered mutation (159)
    Domains in SCOPe 2.08: d6kf1d_
  • Heterogens: NPO, 6NA, PGE, EDO, SO4, MES, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >6kf1A (A:)
tpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlscpgp
agdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdyrla
pehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpadvp
vvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdhtaa
pptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdleri
iaamkmmlg

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >6kf1B (B:)
tpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlscpgp
agdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdyrla
pehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpadvp
vvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdhtaa
pptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdleri
iaamkmmlg

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >6kf1C (C:)
tpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlscpgp
agdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdyrla
pehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpadvp
vvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdhtaa
pptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdleri
iaamkmmlg

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >6kf1D (D:)
dtpfirpdmkafleaiaamagptlaemtleearasyvalhgmadrparelavirnlscpg
pagdiplrlydaresreagpvitfyhgggfvigdldthhnlcteiaalmdlpvvavdyrl
apehpfpaaiedceaatrwvasspselgrtasgvipigdaaggnativvsqllgakpadv
pvvlqvpifplasdavgsasleafaegfvltkasieffdtaykadradprgfpilgdhta
apptivatasldpirdsgrdyakalveagrdvvylemegvthsftniraavpstqgdler
iiaamkmmlg