PDB entry 6kc7

View 6kc7 on RCSB PDB site
Description: crystal structure of nme1cas9 in complex with sgrna and target dna (atatgatt pam) in seed-base paring state
Deposited on 2019-06-27, released 2019-11-06
The last revision was dated 2020-01-08, with a file datestamp of 2020-01-03.
Experiment type: XRAY
Resolution: 3.3 Å
R-factor: N/A
AEROSPACI score: 0.09 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated endonuclease Cas9
    Species: Neisseria meningitidis 8013 [TaxId:604162]
    Gene: cas9, NMV_1993
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9X1G5 (Start-1082)
      • engineered mutation (588)
  • Chain 'B':
    Compound: sgrna
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'C':
    Compound: DNA (5'-d(*tp*ap*ap*ap*ap*tp*cp*ap*tp*ap*tp*gp*tp*ap*ap*ap*gp*tp*t)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'D':
    Compound: DNA (5'-d(*ap*tp*ap*tp*gp*ap*tp*tp*tp*tp*a)-3')
    Species: synthetic construct, synthetic [TaxId:32630]

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6kc7A (A:)
smaafkpnsinyilgldigiasvgwamveideeenpirlidlgvrvferaevpktgdsla
marrlarsvrrltrrrahrllrtrrllkregvlqaanfdenglikslpntpwqlraaald
rkltplewsavllhlikhrgylsqrknegetadkelgallkgvagnahalqtgdfrtpae
lalnkfekesghirnqrsdyshtfsrkdlqaelillfekqkefgnphvsgglkegietll
mtqrpalsgdavqkmlghctfepaepkaakntytaerfiwltklnnlrileqgserpltd
teratlmdepyrkskltyaqarkllgledtaffkglrygkdnaeastlmemkayhaisra
lekeglkdkksplnlspelqdeigtafslfktdeditgrlkdriqpeileallkhisfdk
fvqislkalrrivplmeqgkrydeacaeiygdhygkknteekiylppipadeirnpvvlr
alsqarkvingvvrrygsparihietarevgksfkdrkeiekrqeenrkdrekaaakfre
yfpnfvgepkskdilklrlyeqqhgkclysgkeinlgrlnekgyveidaalpfsrtwdds
fnnkvlvlgsenqnkgnqtpyeyfngkdnsrewqefkarvetsrfprskkqrillqkfde
dgfkernlndtryvnrflcqfvadrmrltgkgkkrvfasngqitnllrgfwglrkvraen
drhhaldavvvacstvamqqkitrfvrykemnafdgktidketgevlhqkthfpqpweff
aqevmirvfgkpdgkpefeeadtleklrtllaeklssrpeavheyvtplfvsrapnrkms
gqghmetvksakrldegvsvlrvpltqlklkdlekmvnrerepklyealkarleahkddp
akafaepfykydkagnrtqqvkavrveqvqktgvwvrnhngiadnatmvrvdvfekgdky
ylvpiyswqvakgilpdravvqgkdeedwqliddsfnfkfslhpndlvevitkkarmfgy
faschrgtgninirihdldhkigkngilegigvktalsfqkyqidelgkeirpcrlkkrp
pvr

    Sequence, based on observed residues (ATOM records):
    >6kc7A (A:)
sinyilgldigiasvgwamveideeenpirlidlgvrvferaevpktgdslamarrlars
vrrltrrrahrllrtrrllkregvlqaanfdenglikslpntpwqlraaaldrkltplew
savllhlikhrgylsqelgallkgvagnahalqtgdfrtpaelalnkfekesghirnqrs
dyshtfsrkdlqaelillfekqkefgnphvsgglkegietllmtqrpalpkaakntytae
rfiwltklnnlrileqgserpltdteratlmdepyrksktyaqarkllastlmemkayha
isrsplnlspelqdeigtafslfktdeditgrlkdriisfdkfvqiekiylppipadeir
npvvlralsqarkvingvvrrygsparihietarevgksfkdrkeiekrqeenrkdreka
aakfreyfpnfvgepkskdilklrlyeqqhgkclysgkeinlgrlnekgyveidaalpfs
rtwddsfnnkvlvlgsenqnkgnqtpyeyfngkdnsrewqefkarvetsrfprskkqril
lqkfdedgfkernlndtryvnrflcqfvadrmrltgkgkkrvfasngqitnllrgfwglr
kvraendrhhaldavvvacstvamqqkitrfvrykemnafdgktidlhqkthfpqpweff
aqevmirvfgkpdgkpefeeadtleklrtllaeklssrpeavheyvtplfvsrapnrkms
gqghmetvksakrldegvsvlrvpltqlklkdlekmvnrerepklyealkarleahkddp
akafaepfykydkagnrtqqvkavrveqvqktgvwvrnhngiadnatmvrvdvfekgdky
ylvpiyswqvakgilpdravvqgkdeedwqliddsfnfkfslhpndlvevitkkarmfgy
faschrgtgninirihdldhkigkngilegigvktalsfqkyqidelgkeirpcrlkkrp
pvr

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.