PDB entry 6k5a

View 6k5a on RCSB PDB site
Description: Crystal structure of the E148D/R147A/F317A mutant in presence of 200 mM NaBr
Class: membrane protein
Keywords: Cl- / H+ antiporter, CLC transporter, MEMBRANE PROTEIN
Deposited on 2019-05-28, released 2019-08-28
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-09-11, with a file datestamp of 2019-09-06.
Experiment type: XRAY
Resolution: 3.16 Å
R-factor: N/A
AEROSPACI score: 0.11 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: H(+)/Cl(-) exchange transporter clcA
    Species: Escherichia coli MS 117-3 [TaxId:749539]
    Gene: clcA, eriC, HMPREF9542_03540
    Database cross-references and differences (RAF-indexed):
    • Uniprot E9TIA0
      • engineered mutation (146-147)
      • engineered mutation (316)
    Domains in SCOPe 2.08: d6k5aa_
  • Chain 'B':
    Compound: H(+)/Cl(-) exchange transporter clcA
    Species: Escherichia coli MS 117-3 [TaxId:749539]
    Gene: clcA, eriC, HMPREF9542_03540
    Database cross-references and differences (RAF-indexed):
    • Uniprot E9TIA0
      • engineered mutation (146-147)
      • engineered mutation (316)
    Domains in SCOPe 2.08: d6k5ab_
  • Chain 'C':
    Compound: Fab fragment, heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6K5A (0-221)
    Domains in SCOPe 2.08: d6k5ac_
  • Chain 'D':
    Compound: Fab fragment, light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6K5A (0-210)
    Domains in SCOPe 2.08: d6k5ad1, d6k5ad2
  • Chain 'E':
    Compound: Fab fragment, heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6K5A (Start-221)
    Domains in SCOPe 2.08: d6k5ae_
  • Chain 'F':
    Compound: Fab fragment, light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 6K5A (0-210)
    Domains in SCOPe 2.08: d6k5af1, d6k5af2
  • Heterogens: BR

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6k5aA (A:)
mktdtpsletpqaarlrrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawl
qnqrmgalvhtadnypllltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqr
pvrwwrvlpvkffgglgtlgggmvlgadgptvqiggnigrmvldifrlkgdearhtllat
gaaaglaaafnaplagilfiieemrpqfrytlisikavfigvimstimyrifnhevalid
vgklsdaplntlwlylilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigg
lcgllgfvapatsggganlipiatagnfsmgmlvfifvarvittllcfssgapggifapm
lalgtvlgtafgmvavelfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyq
lilpmiitglgatllaqftggkplysailartlakqeaeqlarskaasasent

    Sequence, based on observed residues (ATOM records): (download) 
    >6k5aA (A:)
rrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadnyp
llltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffggl
gtlgggmvlgadgptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnaplag
ilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwlyl
ilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsggg
anlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmvav
elfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatlla
qftggkplysailartlakq

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6k5aB (B:)
mktdtpsletpqaarlrrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawl
qnqrmgalvhtadnypllltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqr
pvrwwrvlpvkffgglgtlgggmvlgadgptvqiggnigrmvldifrlkgdearhtllat
gaaaglaaafnaplagilfiieemrpqfrytlisikavfigvimstimyrifnhevalid
vgklsdaplntlwlylilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigg
lcgllgfvapatsggganlipiatagnfsmgmlvfifvarvittllcfssgapggifapm
lalgtvlgtafgmvavelfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyq
lilpmiitglgatllaqftggkplysailartlakqeaeqlarskaasasent

    Sequence, based on observed residues (ATOM records): (download) 
    >6k5aB (B:)
rrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadnyp
llltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffggl
gtlgggmvlgadgptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnaplag
ilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwlyl
ilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsggg
anlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmvav
elfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatlla
qftggkplysailartlakq

  • Chain 'C':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >6k5aC (C:)
evrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstiny
tpslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvs
sakttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqa
alytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >6k5aD (D:)
divltqspaimsaapgdkvtmtcsasssvsyihwyqqksgtspkrwiydtskltsgvpvr
fsgsgsgtsysltintmeaedaatyycqqwsshpqtfgggtkleilradaaptvsifpps
seqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstltl
tkdeyerhnsytceathktstspivksfnra

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >6k5aE (E:)
evrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstiny
tpslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvs
sakttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqa
alytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

    Sequence, based on observed residues (ATOM records): (download) 
    >6k5aE (E:)
vrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstinyt
pslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvss
akttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqaa
lytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >6k5aF (F:)
divltqspaimsaapgdkvtmtcsasssvsyihwyqqksgtspkrwiydtskltsgvpvr
fsgsgsgtsysltintmeaedaatyycqqwsshpqtfgggtkleilradaaptvsifpps
seqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstltl
tkdeyerhnsytceathktstspivksfnra