PDB entry 6k4q

View 6k4q on RCSB PDB site
Description: crystal structure of xcas9 in complex with sgrna and dna (cgg pam)
Deposited on 2019-05-26, released 2019-09-25
The last revision was dated 2019-11-13, with a file datestamp of 2019-11-08.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: N/A
AEROSPACI score: 0.17 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: sgrna
    Species: STREPTOCOCCUS PYOGENES, synthetic [TaxId:1314]
  • Chain 'B':
    Compound: crispr-associated endonuclease cas9/csn1
    Species: Streptococcus pyogenes serotype M1 [TaxId:301447]
    Gene: cas9
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q99ZW2
      • engineered mutation (9)
      • engineered mutation (79)
      • engineered mutation (261)
      • engineered mutation (323)
      • engineered mutation (408)
      • engineered mutation (479)
      • engineered mutation (542)
      • engineered mutation (573)
      • engineered mutation (693)
      • engineered mutation (839)
      • engineered mutation (1218)
  • Chain 'C':
    Compound: DNA (28-mer)
    Species: STREPTOCOCCUS PYOGENES, synthetic [TaxId:1314]
  • Chain 'D':
    Compound: DNA (5'-d(*ap*ap*ap*cp*gp*gp*tp*ap*tp*tp*g)-3')
    Species: STREPTOCOCCUS PYOGENES, synthetic [TaxId:1314]
  • Heterogens: PO4, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    No sequence available.

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6k4qB (B:)
mdkkysiglaigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsgetae
atrlkrtarrrytrrknrilylqeifsnemakvddsffhrleesflveedkkherhpifg
nivdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnpdnsd
vdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfgn
lialslgltpnfksnfdlaedtklqlskdtydddldnllaqigdqyadlflaaknlsdai
llsdilrvnteitkaplsasmiklydehhqdltllkalvrqqlpekykeiffdqskngya
gyidggasqeefykfikpilekmdgteellvklnredllrkqrtfdngiiphqihlgelh
ailrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpwnfek
vvdkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrkpafl
sgdqkkaivdllfktnrkvtvkqlkedyfkkieefdsveisgvedrfnaslgtyhdllki
ikdkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrrytgwg
rlsrklingirdkqsgktildflksdgfanrnfiqlihddsltfkediqkaqvsgqgdsl
hehianlagspaikkgilqtvkvvdelvkvmgrhkpeniviemarenqttqkgqknsrer
mkrieegikelgsqilkehpventqlqneklylyylqngrdmyvdqeldinrlsdydvda
ivpqsflkddsidnkvltrsdknrgksdnvpseevvkkmknywrqllnaklitqrkfdnl
tkaergglseldkagfikrqlvetrqitkhvaqildsrmntkydendklirevkvitlks
klvsdfrkdfqfykvreinnyhhahdaylnavvgtalikkypklesefvygdykvydvrk
miakseqeigkatakyffysnimnffkteitlangeirkrplietngetgeivwdkgrdf
atvrkvlsmpqvnivkktevqtggfskesilpkrnsdkliarkkdwdpkkyggfdsptva
ysvlvvakvekgkskklksvkellgitimerssfeknpidfleakgykevkkdliiklpk
yslfelengrkrmlasagvlqkgnelalpskyvnflylashyeklkgspedneqkqlfve
qhkhyldeiieqisefskrviladanldkvlsaynkhrdkpireqaeniihlftltnlga
paafkyfdttidrkrytstkevldatlihqsitglyetridlsqlggd

    Sequence, based on observed residues (ATOM records):
    >6k4qB (B:)
kkysiglaigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsgetaeat
rlkrtarrrytrrknrilylqeifsnemakvddsffhrleesflveedkkherhpifgni
vdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnpdnsdvd
klfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfgnli
alslgltpnfksnfdlaedtklqlskdtydddldnllaqigdqyadlflaaknlsdaill
sdilrvnteitkaplsasmiklydehhqdltllkalvrqqlpekykeiffdqskngyagy
idggasqeefykfikpilekmdgteellvklnredllrkqrtfdngiiphqihlgelhai
lrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpwnfekvv
dkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrkpaflsg
dqkkaivdllfktnrkvtvkqlkedyfkkieefdsveisgvedrfnaslgtyhdllkiik
dkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrrytgwgrl
srklingirdkqsgktildflksdgfanrnfiqlihddsltfkediqkaqvsslhehian
lagspaikkgilqtvkvvdelvkvmgrhkpeniviemarqknsrermkrieegikelgsq
ilkehpventqlqneklylyylqngrdmyvdqeldinrlsdydvdaivpqsflkddsidn
kvltrsdknrgksdnvpseevvkkmknywrqllnaklitqrkfdnltkaergglseldka
gfikrqlvetrqitkhvaqildsrmntkydendklirevkvitlksklvsdfrkdfqfyk
vreinnyhhahdaylnavvgtalikkypklesefvyggkatakyffysnimnffkteikr
plietngetgeivwdkgrdfatvrkvlsmpqvnivkktevqtggfskesilpkrnsdkli
arkkdwdpkkyggfdsptvaysvlvvakvekgkskklksvkellgitimerssfeknpid
fleakgykevkkdliiklpkyslfelengrkrmlasagvlqkgnelalpskyvnflylas
hyekpedneqkqlfveqhkhyldeiieqisefskrviladanldkvlsaynkhrdkpire
qaeniihlftltnlgapaafkyfdttidrkrytstkevldatlihqsitglyetridlsq
lgg

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.