PDB entry 6jfu

View 6jfu on RCSB PDB site
Description: crystal structure of nme2cas9 in complex with sgrna and target dna (aggccc pam)
Deposited on 2019-02-12, released 2019-11-06
The last revision was dated 2020-01-08, with a file datestamp of 2020-01-03.
Experiment type: XRAY
Resolution: 3.2 Å
R-factor: N/A
AEROSPACI score: 0.1 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated endonuclease Cas9
    Species: Neisseria meningitidis [TaxId:487]
    Gene: cas9
    Database cross-references and differences (RAF-indexed):
    • PDB 6JFU (Start-1082)
  • Chain 'B':
    Compound: sgrna
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'C':
    Compound: target-strand DNA
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'D':
    Compound: non-target strand
    Species: synthetic construct, synthetic [TaxId:32630]
  • Heterogens: EDO

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6jfuA (A:)
    smaafkpnpinyilglaigiasvgwamveideeenpirlidlgvrvferaevpktgdsla
    marrlarsvrrltrrrahrllrarrllkregvlqaadfdenglikslpntpwqlraaald
    rkltplewsavllhlikhrgylsqrknegetadkelgallkgvannahalqtgdfrtpae
    lalnkfekesghirnqrgdyshtfsrkdlqaelillfekqkefgnphvsgglkegietll
    mtqrpalsgdavqkmlghctfepaepkaakntytaerfiwltklnnlrileqgserpltd
    teratlmdepyrkskltyaqarkllgledtaffkglrygkdnaeastlmemkayhaisra
    lekeglkdkksplnlsselqdeigtafslfktdeditgrlkdrvqpeileallkhisfdk
    fvqislkalrrivplmeqgkrydeacaeiygdhygkknteekiylppipadeirnpvvlr
    alsqarkvingvvrrygsparihietarevgksfkdrkeiekrqeenrkdrekaaakfre
    yfpnfvgepkskdilklrlyeqqhgkclysgkeinlvrlnekgyveidaalpfsrtwdds
    fnnkvlvlgsenqnkgnqtpyeyfngkdnsrewqefkarvetsrfprskkqrillqkfde
    dgfkecnlndtryvnrflcqfvadhilltgkgkrrvfasngqitnllrgfwglrkvraen
    drhhaldavvvacstvamqqkitrfvrykemnafdgktidketgkvlhqkthfpqpweff
    aqevmirvfgkpdgkpefeeadtpeklrtllaeklssrpeavheyvtplfvsrapnrkms
    gahkdtlrsakrfvkhnekisvkrvwlteikladlenmvnykngreielyealkarleay
    ggnakqafdpkdnpfykkggqlvkavrvektqesgvllnkknaytiadngdmvrvdvfck
    vdkkgknqyfivpiyawqvaenilpdidckgyriddsytfcfslhkydliafqkdekskv
    efayyincdssngrfylawhdkgskeqqfristqnlvliqkyqvnelgkeirpcrlkkrp
    pvr
    

    Sequence, based on observed residues (ATOM records):
    >6jfuA (A:)
    npinyilglaigiasvgwamveideeenpirlidlgvrvferaevpktgdslamarrlar
    svrrltrrrahrllrarrllkregvlqaadfdenglikslpntpwqlraaaldrkltple
    wsavllhlikhrgylsqrknegetadkelgallkgvannahalqtgdfrtpaelalnkfe
    kesghirnqrgdyshtfsrkdlqaelillfekqkefgnphvsgglkegietllmtqrpal
    sgdavqkmlghctfepaepkaakntytaerfiwltklnnlrileqgserpltdteratlm
    depyrkskltyaqarkllgledtaffkglreastlmemkayhaisralekeglkdkkspl
    nlsselqdeigtafslfktdeditgrlkdrvqpeileallkhisfdkfvqislkalrriv
    plmeqgkrydeacaeiygdhygkknteekiylppipadeirnpvvlralsqarkvingvv
    rrygsparihietarevgksfkdrkeiedtryvnrflcqfvadhivfasngqitnllrgk
    vraendrhhaldavvvacstvamqqkitrfvrykthfpqpweffaqevmirvfgkpdgkp
    efeeadtpeklrtllaeklpeavheyvtplfvsrapnrkmsgahkdtlrsakrfvkhnek
    isvkrvwlteikladlenmvnykngreielyealkarleayggnakqafdpkdnpfykkg
    gqlvkavrvektqesgvllnkknaytiadngdmvrvdvfckvdkkgknqyfivpiyawqv
    aenilpdidckgyriddsytfcfslhkydliafqkdekskvefayyincdssngrfylaw
    hdkgskeqqfristqnlvliqkyqvnelgkeirpcrlkkrppvr
    

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.