PDB entry 6je3

View 6je3 on RCSB PDB site
Description: crystal structure of nme2cas9 in complex with sgrna and target dna (aggccc pam) with 5 nt overhang
Deposited on 2019-02-03, released 2019-11-06
The last revision was dated 2020-01-08, with a file datestamp of 2020-01-03.
Experiment type: XRAY
Resolution: 2.93 Å
R-factor: N/A
AEROSPACI score: 0.13 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated endonuclease Cas9
    Species: Neisseria meningitidis [TaxId:487]
    Gene: cas9
    Database cross-references and differences (RAF-indexed):
    • PDB 6JE3 (Start-1082)
  • Chain 'B':
    Compound: sgrna
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'C':
    Compound: target DNA strand
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'D':
    Compound: non-target DNA strand
    Species: synthetic construct, synthetic [TaxId:32630]
  • Heterogens: EDO, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6je3A (A:)
smaafkpnpinyilglaigiasvgwamveideeenpirlidlgvrvferaevpktgdsla
marrlarsvrrltrrrahrllrarrllkregvlqaadfdenglikslpntpwqlraaald
rkltplewsavllhlikhrgylsqrknegetadkelgallkgvannahalqtgdfrtpae
lalnkfekesghirnqrgdyshtfsrkdlqaelillfekqkefgnphvsgglkegietll
mtqrpalsgdavqkmlghctfepaepkaakntytaerfiwltklnnlrileqgserpltd
teratlmdepyrkskltyaqarkllgledtaffkglrygkdnaeastlmemkayhaisra
lekeglkdkksplnlsselqdeigtafslfktdeditgrlkdrvqpeileallkhisfdk
fvqislkalrrivplmeqgkrydeacaeiygdhygkknteekiylppipadeirnpvvlr
alsqarkvingvvrrygsparihietarevgksfkdrkeiekrqeenrkdrekaaakfre
yfpnfvgepkskdilklrlyeqqhgkclysgkeinlvrlnekgyveidaalpfsrtwdds
fnnkvlvlgsenqnkgnqtpyeyfngkdnsrewqefkarvetsrfprskkqrillqkfde
dgfkecnlndtryvnrflcqfvadhilltgkgkrrvfasngqitnllrgfwglrkvraen
drhhaldavvvacstvamqqkitrfvrykemnafdgktidketgkvlhqkthfpqpweff
aqevmirvfgkpdgkpefeeadtpeklrtllaeklssrpeavheyvtplfvsrapnrkms
gahkdtlrsakrfvkhnekisvkrvwlteikladlenmvnykngreielyealkarleay
ggnakqafdpkdnpfykkggqlvkavrvektqesgvllnkknaytiadngdmvrvdvfck
vdkkgknqyfivpiyawqvaenilpdidckgyriddsytfcfslhkydliafqkdekskv
efayyincdssngrfylawhdkgskeqqfristqnlvliqkyqvnelgkeirpcrlkkrp
pvr

    Sequence, based on observed residues (ATOM records):
    >6je3A (A:)
npinyilglaigiasvgwamveideeenpirlidlgvrvferaevpktgdslamarrlar
svrrltrrrahrllrarrllkregvlqaadfdenglikslpntpwqlraaaldrkltple
wsavllhlikhrgylsqrkkelgallkgvannahalqtgdfrtpaelalnkfekesghir
nqrgdyshtfsrkdlqaelillfekqkefgnphvsgglkegietllmtqrpalsgdavqk
mlghctfepaepkaakntytaerfiwltklnnlrileqpltdteratlmdepyrksklty
aqarkllgledtaffkglrygkdnaeastlmemkayhaisralekeglkdkksplnlsse
lqdeigtafslfktdeditgrlkdrvqpeileallkhisfdkfvqislkalrrivplmeq
gkrydeacaeiygdhyteekiylppipadeirnpvvlralsqarkvingvvrrygspaih
ietarevgksfkdrkeieflcqfvadhilltgkgkrrvfasngqitnllrgendrhhald
avvvactvampweffaqevmirvfgkpdgkpefeeadtpeklrtllaeklssrpeavhey
vtplfvsrapnrkmsgahkdtlrsakrfvkhnekisvkrvwlteikladlenmvnykngr
eielyealkarleayggnakqafdpkdnpfykkggqlvkavrvektqesgvllnkknayt
iadngdmvrvdvfckvdkkgknqyfivpiyawqvaenilpdidckgyriddsytfcfslh
kydliafqkdekskvefayyincdssngrfylawhdkgskeqqfristqnlvliqkyqvn
elgkeirpcrlkkrppvr

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.