PDB entry 6j35

View 6j35 on RCSB PDB site
Description: Crystal structure of ligand-free of PulA-G680L mutant from Klebsiella pneumoniae
Class: hydrolase
Keywords: Pullulanase, Klebsiella pneumoniae, ligand-free, G680, HYDROLASE
Deposited on 2019-01-09, released 2019-09-18
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-09-18, with a file datestamp of 2019-09-13.
Experiment type: XRAY
Resolution: 1.84 Å
R-factor: N/A
AEROSPACI score: 0.35 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: pullulanase
    Species: Klebsiella pneumoniae [TaxId:573]
    Gene: pulA
    Database cross-references and differences (RAF-indexed):
    • Uniprot W9BQ28 (1-1052)
      • see sequence details (198)
      • engineered mutation (649)
    Domains in SCOPe 2.08: d6j35a1, d6j35a2, d6j35a3, d6j35a4, d6j35a5
  • Chain 'B':
    Compound: pullulanase
    Species: Klebsiella pneumoniae [TaxId:573]
    Gene: pulA
    Database cross-references and differences (RAF-indexed):
    • Uniprot W9BQ28 (1-1052)
      • initiating methionine (0)
      • see sequence details (198)
      • engineered mutation (649)
    Domains in SCOPe 2.08: d6j35b1, d6j35b2, d6j35b3, d6j35b4, d6j35b5
  • Heterogens: MG, GOL, CA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6j35A (A:)
mdvvvrlpdvavpgeavqasarqavihlvdiagitsstpadyatknlylwnnetcdalsa
pvadwndvsttptgsdkygpywvipltkesgcinvivrdgtnklidsdlrvsfsdftdrt
vsviagnsavydsradafraafgvaladahwvdkttllwpggenkpivrlyyshsskvaa
dsngefsdkyvkltpttvsqqvsmrfphlasypafklpddvnvdellqgetvaiaaesdg
ilssatqvqtagvlddtyaaaaealsygaqltdsgvtfrvwaptaqqvelviysadkkvi
ashpmtrdsasgawswqggsdlkgafyryamtvyhpqsrkveqyevtdpyahslstnsey
sqvvdlndsalkpegwdgltmphaqktkadlakmtiheshirdlsawdqtvpaelrgkyl
altaqesnmvqhlkqlsasgvthiellpvfdlatvnefsdkvadiqqpfsrlcevnsavk
ssefagycdsgstveevltqlkqndskdnpqvqalntlvaqtdsynwgydpfhytvpegs
yatdpegtarikefrtmiqaikqdlgmnvimdvvynhtnaagptdrtsvldkivpwyyqr
lnettgsvesatccsdsapehrmfakliadslavwttdykidgfrfdlmlyhpkaqilsa
werikalnpdiyffgegwdsnqsdrfeiasqinlkgtgigtfsdrlrdavrgggpfdsgd
alrqnqgvgsgagvlpnelttlsddqarhladltrlgmagnladfvlidkdgavkrgsei
dyngapggyaadptevvnyvskhdnqtlwdmisykaaqeadldtrvrmqavslatvmlgq
giafdqqgsellrsksftrdsydsgdwfnrvdyslqdnnynvgmprssddgsnydiiarv
kdavatpgetelkqmtafyqeltalrkssplftlgdgatvmkrvdfrntgadqqtgllvm
tiddgmqagasldsrvdgivvainaapesrtlqdfagtslqlsaiqqaagdrslasgvqv
aadgsvtlpawsvavlelpqgesqgaglpvssk

    Sequence, based on observed residues (ATOM records): (download) 
    >6j35A (A:)
dvvvrlpdvavpgeavqasarqavihlvdiagitsstpadyatknlylwnnetcdalsap
vadwndvsttptgsdkygpywvipltkesgcinvivrdgtnklidsdlrvsfsdftdrtv
sviagnsavydsradafraafgvaladahwvdkttllwpggenkpivrlyyshsskvaad
sngefsdkyvkltpttvsqqvsmrfphlasypafklpddvnvdellqgetvaiaaesdgi
lssatqvqtagvlddtyaaaaealsygaqltdsgvtfrvwaptaqqvelviysadkkvia
shpmtrdsasgawswqggsdlkgafyryamtvyhpqsrkveqyevtdpyahslstnseys
qvvdlndsalkpegwdgltmphaqktkadlakmtiheshirdlsawdqtvpaelrgkyla
ltaqesnmvqhlkqlsasgvthiellpvfdlatvnefsdkvadiqqpfsrlcevnsavks
sefagycdsgstveevltqlkqndskdnpqvqalntlvaqtdsynwgydpfhytvpegsy
atdpegtarikefrtmiqaikqdlgmnvimdvvynhtnaagptdrtsvldkivpwyyqrl
nettgsvesatccsdsapehrmfakliadslavwttdykidgfrfdlmlyhpkaqilsaw
erikalnpdiyffgegwdsnqsdrfeiasqinlkgtgigtfsdrlrdavrgggpfdsgda
lrqnqgvgsgagvlpnelttlsddqarhladltrlgmagnladfvlidkdgavkrgseid
yngapggyaadptevvnyvskhdnqtlwdmisykaaqeadldtrvrmqavslatvmlgqg
iafdqqgsellrsksftrdsydsgdwfnrvdyslqdnnynvgmprssddgsnydiiarvk
davatpgetelkqmtafyqeltalrkssplftlgdgatvmkrvdfrntgadqqtgllvmt
iddgmqagasldsrvdgivvainaapesrtlqdfagtslqlsaiqqaagdrslasgvqva
adgsvtlpawsvavlelpqgesqgaglpvssk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6j35B (B:)
mdvvvrlpdvavpgeavqasarqavihlvdiagitsstpadyatknlylwnnetcdalsa
pvadwndvsttptgsdkygpywvipltkesgcinvivrdgtnklidsdlrvsfsdftdrt
vsviagnsavydsradafraafgvaladahwvdkttllwpggenkpivrlyyshsskvaa
dsngefsdkyvkltpttvsqqvsmrfphlasypafklpddvnvdellqgetvaiaaesdg
ilssatqvqtagvlddtyaaaaealsygaqltdsgvtfrvwaptaqqvelviysadkkvi
ashpmtrdsasgawswqggsdlkgafyryamtvyhpqsrkveqyevtdpyahslstnsey
sqvvdlndsalkpegwdgltmphaqktkadlakmtiheshirdlsawdqtvpaelrgkyl
altaqesnmvqhlkqlsasgvthiellpvfdlatvnefsdkvadiqqpfsrlcevnsavk
ssefagycdsgstveevltqlkqndskdnpqvqalntlvaqtdsynwgydpfhytvpegs
yatdpegtarikefrtmiqaikqdlgmnvimdvvynhtnaagptdrtsvldkivpwyyqr
lnettgsvesatccsdsapehrmfakliadslavwttdykidgfrfdlmlyhpkaqilsa
werikalnpdiyffgegwdsnqsdrfeiasqinlkgtgigtfsdrlrdavrgggpfdsgd
alrqnqgvgsgagvlpnelttlsddqarhladltrlgmagnladfvlidkdgavkrgsei
dyngapggyaadptevvnyvskhdnqtlwdmisykaaqeadldtrvrmqavslatvmlgq
giafdqqgsellrsksftrdsydsgdwfnrvdyslqdnnynvgmprssddgsnydiiarv
kdavatpgetelkqmtafyqeltalrkssplftlgdgatvmkrvdfrntgadqqtgllvm
tiddgmqagasldsrvdgivvainaapesrtlqdfagtslqlsaiqqaagdrslasgvqv
aadgsvtlpawsvavlelpqgesqgaglpvssk

    Sequence, based on observed residues (ATOM records): (download) 
    >6j35B (B:)
mdvvvrlpdvavpgeavqasarqavihlvdiadyatknlylwnnetcdalsapvadwndv
sttptgsdkygpywvipltkesgcinvivrdgtnklidsdlrvsfsdftdrtvsviagns
avydsradafraafgvaladahwvdkttllwpggenkpivrlyyshsskvaadsngefsd
kyvkltpttvsqqvsmrfphlasypafklpddvnvdellqgetvaiaaesdgilssatqv
qtagvlddtyaaaaealsygaqltdsgvtfrvwaptaqqvelviysadkkviashpmtrd
sasgawswqggsdlkgafyryamtvyhpqsrkveqyevtdpyahslstnseysqvvdlnd
salkpegwdgltmphaqktkadlakmtiheshirdlsawdqtvpaelrgkylaltaqesn
mvqhlkqlsasgvthiellpvfdlatvnefsdkvadiqqpfsrlcevnsavkssefagyc
dsgstveevltqlkqndskdnpqvqalntlvaqtdsynwgydpfhytvpegsyatdpegt
arikefrtmiqaikqdlgmnvimdvvynhtnaagptdrtsvldkivpwyyqrlnettgsv
esatccsdsapehrmfakliadslavwttdykidgfrfdlmlyhpkaqilsawerikaln
pdiyffgegwdsnqsdrfeiasqinlkgtgigtfsdrlrdavrgggpfdsgdalrqnqgv
gsgagvlpnelttlsddqarhladltrlgmagnladfvlidkdgavkrgseidyngapgg
yaadptevvnyvskhdnqtlwdmisykaaqeadldtrvrmqavslatvmlgqgiafdqqg
sellrsksftrdsydsgdwfnrvdyslqdnnynvgmprssddgsnydiiarvkdavatpg
etelkqmtafyqeltalrkssplftlgdgatvmkrvdfrntgadqqtgllvmtiddgmqa
gasldsrvdgivvainaapesrtlqdfagtslqlsaiqqaagdrslasgvqvaadgsvtl
pawsvavlelpqgesqgaglpvssk