PDB entry 6isr

View 6isr on RCSB PDB site
Description: structure of lipase mutant with Cys-His-Asp catalytic triad
Class: hydrolase
Keywords: calb, hydrolase
Deposited on 2018-11-18, released 2019-07-24
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-08-07, with a file datestamp of 2019-08-02.
Experiment type: XRAY
Resolution: 2.6 Å
R-factor: N/A
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: lipase b
    Species: Pseudozyma antarctica [TaxId:84753]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P41365 (Start-320)
      • engineered mutation (60)
      • engineered mutation (92)
      • engineered mutation (107-108)
      • engineered mutation (152)
      • engineered mutation (284-285)
    Domains in SCOPe 2.08: d6isra_
  • Chain 'B':
    Compound: lipase b
    Species: Pseudozyma antarctica [TaxId:84753]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P41365 (Start-320)
      • engineered mutation (60)
      • engineered mutation (92)
      • engineered mutation (107-108)
      • engineered mutation (152)
      • engineered mutation (284-285)
    Domains in SCOPe 2.08: d6isrb_
  • Heterogens: NI, PEG, PG4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6isrA (A:)
gamalpsgsdpafsqpksvldagltcqgaspssvskpillvpgtgttgpqsfdsnwipls
aqlgytpcwispppfmlndtqvnteymvnaittlyagsgnnklpvltvcqgglvaqwglt
ffpsirskvdrlmafapdykgtvlagpldalagsapsvwqqttgsalttalrnaggltqi
vpttnlysatdeivqpqvsnspldssylfngknvqaqavcgplfvidhagsltsqfsyvv
grsalrsttgqarsadygitdcnplpandltpeqkvaaaallapyyaaivagpkqncepd
lmpyarpfavgkrtcsgivtp

    Sequence, based on observed residues (ATOM records): (download) 
    >6isrA (A:)
gsdpafsqpksvldagltcqgaspssvskpillvpgtgttgpqsfdsnwiplsaqlgytp
cwispppfmlndtqvnteymvnaittlyagsgnnklpvltvcqgglvaqwgltffpsirs
kvdrlmafapdykgtvlagpldalagsapsvwqqttgsalttalrnaggltqivpttnly
satdeivqpqvsnspldssylfngknvqaqavcgplfvidhagsltsqfsyvvgrsalrs
ttgqarsadygitdcnplpandltpeqkvaaaallapyyaaivagpkqncepdlmpyarp
favgkrtcsgivtp

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6isrB (B:)
gamalpsgsdpafsqpksvldagltcqgaspssvskpillvpgtgttgpqsfdsnwipls
aqlgytpcwispppfmlndtqvnteymvnaittlyagsgnnklpvltvcqgglvaqwglt
ffpsirskvdrlmafapdykgtvlagpldalagsapsvwqqttgsalttalrnaggltqi
vpttnlysatdeivqpqvsnspldssylfngknvqaqavcgplfvidhagsltsqfsyvv
grsalrsttgqarsadygitdcnplpandltpeqkvaaaallapyyaaivagpkqncepd
lmpyarpfavgkrtcsgivtp

    Sequence, based on observed residues (ATOM records): (download) 
    >6isrB (B:)
gsdpafsqpksvldagltcqgaspssvskpillvpgtgttgpqsfdsnwiplsaqlgytp
cwispppfmlndtqvnteymvnaittlyagsgnnklpvltvcqgglvaqwgltffpsirs
kvdrlmafapdykgtvlagpldalagsapsvwqqttgsalttalrnaggltqivpttnly
satdeivqpqvsnspldssylfngknvqaqavcgplfvidhagsltsqfsyvvgrsalrs
ttgqarsadygitdcnplpandltpeqkvaaaallapyyaaivagpkqncepdlmpyarp
favgkrtcsgivtp