PDB entry 6is7

View 6is7 on RCSB PDB site
Description: structure of 9n-i dna polymerase incorporation with da in the active site
Deposited on 2018-11-15, released 2019-08-28
The last revision was dated 2019-08-28, with a file datestamp of 2019-08-23.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: N/A
AEROSPACI score: 0.16 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA polymerase
    Species: Thermococcus sp. 9oN-7 [TaxId:103799]
    Gene: pol, polA
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q56366 (0-End)
      • engineered mutation (140)
      • engineered mutation (142)
      • engineered mutation (484)
  • Chain 'B':
    Compound: DNA polymerase
    Species: Thermococcus sp. 9oN-7 [TaxId:103799]
    Gene: pol, polA
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q56366 (0-End)
      • engineered mutation (140)
      • engineered mutation (142)
      • engineered mutation (484)
  • Chain 'C':
    Compound: DNA (5'-d(p*gp*cp*gp*gp*ap*cp*tp*gp*cp*tp*tp*ap*cp*cp*a)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'D':
    Compound: DNA (5'-d(*ap*cp*tp*gp*gp*tp*ap*ap*gp*cp*ap*gp*tp*cp*cp*gp*cp*g)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'E':
    Compound: DNA (5'-d(p*gp*cp*gp*gp*ap*cp*tp*gp*cp*tp*tp*ap*cp*cp*a)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'F':
    Compound: DNA (5'-d(*ap*cp*tp*gp*gp*tp*ap*ap*gp*cp*ap*gp*tp*cp*cp*gp*cp*g)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'I':
    Compound: DNA (5'-d(*ap*cp*tp*gp*gp*tp*ap*ap*gp*cp*ap*gp*tp*cp*cp*gp*cp*g)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'K':
    Compound: DNA (5'-d(p*gp*cp*gp*gp*ap*cp*tp*gp*cp*tp*tp*ap*cp*cp*a)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'L':
    Compound: DNA (5'-d(p*gp*cp*gp*gp*ap*cp*tp*gp*cp*tp*tp*ap*cp*cp*a)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Heterogens: CA, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6is7A (A:)
mildtdyitengkpvirvfkkengefkieydrtfepyfyallkddsaiedvkkvtakrhg
tvvkvkraekvqkkflgrpievwklyfnhpqdvpairdrirahpavvdiyeydipfakry
lidkglipmegdeeltmlafaiatlyhegeefgtgpilmisyadgsearvitwkkidlpy
vdvvstekemikrflrvvrekdpdvlityngdnfdfaylkkrceelgikftlgrdgsepk
iqrmgdrfavevkgrihfdlypvirrtinlptytleavyeavfgkpkekvyaeeiaqawe
sgeglervarysmedakvtyelgreffpmeaqlsrligqslwdvsrsstgnlvewfllrk
aykrnelapnkpderelarrrggyaggyvkeperglwdnivyldfrslypsiiithnvsp
dtlnregckeydvapevghkfckdfpgfipsllgdlleerqkikrkmkatvdplekklld
yrqrlikilansfygyygyakarwyckecaesvtawgreyiemvireleekfgfkvlyad
tdglhatipgadaetvkkkakeflkyinpklpglleleyegfyvrgffvtkkkyavidee
gkittrgleivrrdwseiaketqarvleailkhgdveeavrivkevteklskyevppekl
viheqitrdlrdykatgphvavakrlaargvkirpgtvisyivlkgsgrigdraipadef
dptkhrydaeyyienqvlpaverilkafgyrkedlryqktkqvglgawlkvkgkklehhh
hhh

    Sequence, based on observed residues (ATOM records):
    >6is7A (A:)
mildtdyitengkpvirvfkkengefkieydrtfepyfyallkddsaiedvkkvtakrhg
tvvkvkraekvqkkflgrpievwklyfnhpqdvpairdrirahpavvdiyeydipfakry
lidkglipmegdeeltmlafaiatlyhegeefgtgpilmisyadgsearvitwkkidlpy
vdvvstekemikrflrvvrekdpdvlityngdnfdfaylkkrceelgikftlgrdgsepk
iqrmgdrfavevkgrihfdlypvirrtinlptytleavyeavfgkpkekvyaeeiaqawe
sgeglervarysmedakvtyelgreffpmeaqlsrligqslwdvsrsstgnlvewfllrk
aykrnelapnkpderelarrrggyaggyvkeperglwdnivyldfrslypsiiithnvsp
dtlnregckeydvapevghkfckdfpgfipsllgdlleerqkikrkmkatvdplekklld
yrqrlikilansfygyygyakarwyckecaesvtawgreyiemvireleekfgfkvlyad
tdglhatipgadaetvkkkakeflkyinpklpglleleyegfyvrgffvtkkkyavidee
gkittrgleivrrdwseiaketqarvleailkhgdveeavrivkevteklskyevppekl
viheqitrdlrdykatgphvavakrlaargvkirpgtvisyivlkgsgrigdraipadef
dptkhrydaeyyienqvlpaverilkafgyrkedlry

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6is7B (B:)
mildtdyitengkpvirvfkkengefkieydrtfepyfyallkddsaiedvkkvtakrhg
tvvkvkraekvqkkflgrpievwklyfnhpqdvpairdrirahpavvdiyeydipfakry
lidkglipmegdeeltmlafaiatlyhegeefgtgpilmisyadgsearvitwkkidlpy
vdvvstekemikrflrvvrekdpdvlityngdnfdfaylkkrceelgikftlgrdgsepk
iqrmgdrfavevkgrihfdlypvirrtinlptytleavyeavfgkpkekvyaeeiaqawe
sgeglervarysmedakvtyelgreffpmeaqlsrligqslwdvsrsstgnlvewfllrk
aykrnelapnkpderelarrrggyaggyvkeperglwdnivyldfrslypsiiithnvsp
dtlnregckeydvapevghkfckdfpgfipsllgdlleerqkikrkmkatvdplekklld
yrqrlikilansfygyygyakarwyckecaesvtawgreyiemvireleekfgfkvlyad
tdglhatipgadaetvkkkakeflkyinpklpglleleyegfyvrgffvtkkkyavidee
gkittrgleivrrdwseiaketqarvleailkhgdveeavrivkevteklskyevppekl
viheqitrdlrdykatgphvavakrlaargvkirpgtvisyivlkgsgrigdraipadef
dptkhrydaeyyienqvlpaverilkafgyrkedlryqktkqvglgawlkvkgkklehhh
hhh

    Sequence, based on observed residues (ATOM records):
    >6is7B (B:)
mildtdyitengkpvirvfkkengefkieydrtfepyfyallkddsaiedvkkvtakrhg
tvvkvkraekvqkkflgrpievwklyfnhpqdvpairdrirahpavvdiyeydipfakry
lidkglipmegdeeltmlafaiatlyhegeefgtgpilmisyadgsearvitwkkidlpy
vdvvstekemikrflrvvrekdpdvlityngdnfdfaylkkrceelgikftlgrdgsepk
iqrmgdrfavevkgrihfdlypvirrtinlptytleavyeavfgkpkekvyaeeiaqawe
sgeglervarysmedakvtyelgreffpmeaqlsrligqslwdvsrsstgnlvewfllrk
aykrnelapnkpderelarrrggyaggyvkeperglwdnivyldfrslypsiiithnvsp
dtlnregckeydvapevghkfckdfpgfipsllgdlleerqkikrkmkatvdplekklld
yrqrlikilansfygyygyakarwyckecaesvtawgreyiemvireleekfgfkvlyad
tdglhatipgadaetvkkkakeflkyinpklpglleleyegfyvrgffvtkkkyavidee
gkittrgleivrrdwseiaketqarvleailkhgdveeavrivkevteklskyevppekl
viheqitrdlrdykatgphvavakrlaargvkirpgtvisyivlkgsgrigdraipadef
dptkhrydaeyyienqvlpaverilkafgyrkedlr

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.

  • Chain 'I':
    No sequence available.

  • Chain 'K':
    No sequence available.

  • Chain 'L':
    No sequence available.