PDB entry 6ifo

View 6ifo on RCSB PDB site
Description: crystal structure of acriia2-spycas9-sgrna ternary complex
Deposited on 2018-09-20, released 2019-01-23
The last revision was dated 2019-02-20, with a file datestamp of 2019-02-15.
Experiment type: XRAY
Resolution: 3.31 Å
R-factor: N/A
AEROSPACI score: 0.1 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: crispr-associated endonuclease cas9/csn1
    Species: Streptococcus pyogenes serotype M1 [TaxId:301447]
    Gene: cas9, csn1, SPy_1046
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q99ZW2 (1-1368)
      • expression tag (0)
  • Chain 'B':
    Compound: crispr-associated endonuclease cas9/csn1
    Species: Streptococcus pyogenes serotype M1 [TaxId:301447]
    Gene: cas9, csn1, SPy_1046
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q99ZW2 (1-1368)
      • expression tag (0)
  • Chain 'C':
    Compound: RNA (99-mer)
    Species: Streptococcus pyogenes M1 GAS [TaxId:160490]
  • Chain 'D':
    Compound: RNA (99-mer)
    Species: Streptococcus pyogenes M1 GAS [TaxId:160490]
  • Chain 'E':
    Compound: AcrIIA2
    Species: Listeria monocytogenes [TaxId:1639]
    Database cross-references and differences (RAF-indexed):
    • PDB 6IFO (Start-122)
  • Chain 'F':
    Compound: AcrIIA2
    Species: Listeria monocytogenes [TaxId:1639]
    Database cross-references and differences (RAF-indexed):
    • PDB 6IFO

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6ifoA (A:)
smdkkysigldigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsgeta
eatrlkrtarrrytrrknricylqeifsnemakvddsffhrleesflveedkkherhpif
gnivdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnpdns
dvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfg
nlialslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknlsda
illsdilrvnteitkaplsasmikrydehhqdltllkalvrqqlpekykeiffdqskngy
agyidggasqeefykfikpilekmdgteellvklnredllrkqrtfdngsiphqihlgel
hailrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpwnfe
evvdkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrkpaf
lsgeqkkaivdllfktnrkvtvkqlkedyfkkiecfdsveisgvedrfnaslgtyhdllk
iikdkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrrytgw
grlsrklingirdkqsgktildflksdgfanrnfmqlihddsltfkediqkaqvsgqgds
lhehianlagspaikkgilqtvkvvdelvkvmgrhkpeniviemarenqttqkgqknsre
rmkrieegikelgsqilkehpventqlqneklylyylqngrdmyvdqeldinrlsdydvd
hivpqsflkddsidnkvltrsdknrgksdnvpseevvkkmknywrqllnaklitqrkfdn
ltkaergglseldkagfikrqlvetrqitkhvaqildsrmntkydendklirevkvitlk
sklvsdfrkdfqfykvreinnyhhahdaylnavvgtalikkypklesefvygdykvydvr
kmiakseqeigkatakyffysnimnffkteitlangeirkrplietngetgeivwdkgrd
fatvrkvlsmpqvnivkktevqtggfskesilpkrnsdkliarkkdwdpkkyggfdsptv
aysvlvvakvekgkskklksvkellgitimerssfeknpidfleakgykevkkdliiklp
kyslfelengrkrmlasagelqkgnelalpskyvnflylashyeklkgspedneqkqlfv
eqhkhyldeiieqisefskrviladanldkvlsaynkhrdkpireqaeniihlftltnlg
apaafkyfdttidrkrytstkevldatlihqsitglyetridlsqlggd

    Sequence, based on observed residues (ATOM records):
    >6ifoA (A:)
smdkkysigldigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsgeta
eatrlkrtarrrytrrknricylqeifsnemakvddsffhrleesflveedkkherhpif
gnivdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnpdns
dvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfg
nlialslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknlsda
illsdilrvnteitkaplsasmikrydehhqdltllkalvrqqlpekykeiffdqskngy
agyidggasqeefykfikpilekmdgteellvklnredllrkqrtfdngsiphqihlgel
hailrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpwnfe
evvdkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrkpaf
lsgeqkkaivdllfktnrkvtvkqlkedyfkkiecfdsveisgvedrfnaslgtyhdllk
iikdkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrrytgw
grlsrklingirdkqsgktildflksdgfanrnfmqlihddsltfkediqkaqvsdslhe
hianlagspaikkgilqtvkvvdelvkvmgrhkpeniviemarenqttqknsrermkrie
egikelgsqilkehpventqlqneklylyylqngrdmyvdqeldinrlsdydvdhivpqs
flkddsidnkvltrsdknrgksdnvpseevvkkmknywrqllnaklitqrkfdnltkaer
gglseldkagfikrqlvetrqitkhvaqildsrmntkydendklirevkvitlksklvsd
frkdfqfykvreinnyhhahdaylnavvgtalikkypklesefvygdykvydvrkmiaks
eqeitakyffysnimnffkteitlangeirkrplietngetgeivwdkgrdfatvrkvls
mpqvnivkktevqtggfskesilpkrnsdkliarkkdwdpkkyggfdsptvaysvlvvak
vekgkskklksvkellgitimerssfeknpidfleakgykevkkdliiklpkyslfelen
grkrmlasagelqkgnelalpskyvnflylashyeklkgspedneqkqlfveqhkhylde
iieqisefskrviladanldkvlsaynkhrdkpireqaeniihlftltnlgapaafkyfd
ttidrkrytstkevldatlihqsitglyetridlsqlggd

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6ifoB (B:)
smdkkysigldigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsgeta
eatrlkrtarrrytrrknricylqeifsnemakvddsffhrleesflveedkkherhpif
gnivdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnpdns
dvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfg
nlialslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknlsda
illsdilrvnteitkaplsasmikrydehhqdltllkalvrqqlpekykeiffdqskngy
agyidggasqeefykfikpilekmdgteellvklnredllrkqrtfdngsiphqihlgel
hailrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpwnfe
evvdkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrkpaf
lsgeqkkaivdllfktnrkvtvkqlkedyfkkiecfdsveisgvedrfnaslgtyhdllk
iikdkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrrytgw
grlsrklingirdkqsgktildflksdgfanrnfmqlihddsltfkediqkaqvsgqgds
lhehianlagspaikkgilqtvkvvdelvkvmgrhkpeniviemarenqttqkgqknsre
rmkrieegikelgsqilkehpventqlqneklylyylqngrdmyvdqeldinrlsdydvd
hivpqsflkddsidnkvltrsdknrgksdnvpseevvkkmknywrqllnaklitqrkfdn
ltkaergglseldkagfikrqlvetrqitkhvaqildsrmntkydendklirevkvitlk
sklvsdfrkdfqfykvreinnyhhahdaylnavvgtalikkypklesefvygdykvydvr
kmiakseqeigkatakyffysnimnffkteitlangeirkrplietngetgeivwdkgrd
fatvrkvlsmpqvnivkktevqtggfskesilpkrnsdkliarkkdwdpkkyggfdsptv
aysvlvvakvekgkskklksvkellgitimerssfeknpidfleakgykevkkdliiklp
kyslfelengrkrmlasagelqkgnelalpskyvnflylashyeklkgspedneqkqlfv
eqhkhyldeiieqisefskrviladanldkvlsaynkhrdkpireqaeniihlftltnlg
apaafkyfdttidrkrytstkevldatlihqsitglyetridlsqlggd

    Sequence, based on observed residues (ATOM records):
    >6ifoB (B:)
smdkkysigldigtnsvgwavitdeykvpskkfkvlgntdrhsikknligallfdsgeta
eatrlkrtarrrytrrknricylqeifsnemakvddsffhrleesflveedkkherhpif
gnivdevayhekyptiyhlrkklvdstdkadlrliylalahmikfrghfliegdlnpdns
dvdklfiqlvqtynqlfeenpinasgvdakailsarlsksrrlenliaqlpgekknglfg
nlialslgltpnfksnfdlaedaklqlskdtydddldnllaqigdqyadlflaaknlsda
illsdilrvnteitkaplsasmikrydehhqdltllkalvrqqlpekykeiffdqskngy
agyidggasqeefykfikpilekmdgteellvklnredllrkqrtfdngsiphqihlgel
hailrrqedfypflkdnrekiekiltfripyyvgplargnsrfawmtrkseetitpwnfe
evvdkgasaqsfiermtnfdknlpnekvlpkhsllyeyftvyneltkvkyvtegmrkpaf
lsgeqkkaivdllfktnrkvtvkqlkedyfkkiecfdsveisgvedrfnaslgtyhdllk
iikdkdfldneenediledivltltlfedremieerlktyahlfddkvmkqlkrrrytgw
grlsrklingirdkqsgktildflksdgfanrnfmqlihddsltfkediqkaqvsdslhe
hianlagspaikkgilqtvkvvdelvkvmgrhkpeniviemarenqttqknsrermkrie
egikelgsqilkehpventqlqneklylyylqngrdmyvdqeldinrlsdydvdhivpqs
flkddsidnkvltrsdknrgksdnvpseevvkkmknywrqllnaklitqrkfdnltkaer
gglseldkagfikrqlvetrqitkhvaqildsrmntkydendklirevkvitlksklvsd
frkdfqfykvreinnyhhahdaylnavvgtalikkypklesefvygdykvydvrkmiaks
eqeitakyffysnimnffkteitlangeirkrplietngetgeivwdkgrdfatvrkvls
mpqvnivkktevqtggfskesilpkrnsdkliarkkdwdpkkyggfdsptvaysvlvvak
vekgkskklksvkellgitimerssfeknpidfleakgykevkkdliiklpkyslfelen
grkrmlasagelqkgnelalpskyvnflylashyeklkgspedneqkqlfveqhkhylde
iieqisefskrviladanldkvlsaynkhrdkpireqaeniihlftltnlgapaafkyfd
ttidrkrytstkevldatlihqsitglyetridlsqlggd

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6ifoE (E:)
mtltraqkkyaeamhefinmvddfeestpdfakevlhdsdyvvitknekyavalcslstd
eceydtnlyldeklvdystvdvngvtyyinivetndiddleiatdedemksgnqeiilks
elk

    Sequence, based on observed residues (ATOM records):
    >6ifoE (E:)
tltraqkkyaeamhefinmvddfeestpdfakevlhdsdyvvitknekyavalcslstnl
yldeklvdystvdvngvtyyinivetndiddleiatdedemksgnqeiilkselk

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6ifoF (F:)
mtltraqkkyaeamhefinmvddfeestpdfakevlhdsdyvvitknekyavalcslstd
eceydtnlyldeklvdystvdvngvtyyinivetndiddleiatdedemksgnqeiilks
elk

    Sequence, based on observed residues (ATOM records):
    >6ifoF (F:)
tltraqkkyaeamhefinmvddfeestpdfakevlhdsdyvvitknekyavalcslstde
ydtnlyldeklvdystvdvngvtyyinivetndiddleiatdedemksgnqeiilkse