PDB entry 6i5u

View 6i5u on RCSB PDB site
Description: cryo-em informed directed evolution of nitrilase 4 leads to a change in quaternary structure.
Deposited on 2018-11-14, released 2019-11-20
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 3.9 Å
R-factor: N/A
AEROSPACI score: 0.06 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6i5uA (A:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i5uA (A:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6i5uB (B:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i5uB (B:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6i5uC (C:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i5uC (C:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6i5uD (D:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i5uD (D:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6i5uE (E:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i5uE (E:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6i5uF (F:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i5uF (F:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts