PDB entry 6i1l

View 6i1l on RCSB PDB site
Description: crystal structure of fncas12a in complex with a crrna guide and ssdna target
Deposited on 2018-10-29, released 2019-01-23
The last revision was dated 2019-02-20, with a file datestamp of 2019-02-15.
Experiment type: XRAY
Resolution: 2.98 Å
R-factor: N/A
AEROSPACI score: 0.15 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated endonuclease Cas12a
    Species: Francisella tularensis subsp. novicida (strain U112) [TaxId:401614]
    Gene: cas12a, cpf1, FTN_1397
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0Q7Q2 (2-End)
      • expression tag (0-1)
      • engineered mutation (1006)
  • Chain 'B':
    Compound: crRNA (40-MER)
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'C':
    Compound: ssDNA target strand
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'D':
    Compound: CRISPR-associated endonuclease Cas12a
    Species: Francisella tularensis subsp. novicida (strain U112) [TaxId:401614]
    Gene: cas12a, cpf1, FTN_1397
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0Q7Q2 (2-1300)
      • expression tag (1)
      • engineered mutation (1006)
  • Chain 'E':
    Compound: crRNA (40-MER)
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'F':
    Compound: ssDNA target strand
    Species: synthetic construct, synthetic [TaxId:32630]
  • Heterogens: MG, CIT, K, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6i1lA (A:)
nasiyqefvnkyslsktlrfelipqgktlenikarglilddekrakdykkakqiidkyhq
ffieeilssvcisedllqnysdvyfklkksdddnlqkdfksakdtikkqiseyikdsekf
knlfnqnlidakkgqesdlilwlkqskdngielfkansditdidealeiiksfkgwttyf
kgfhenrknvyssndiptsiiyrivddnlpkflenkakyeslkdkapeainyeqikkdla
eeltfdidyktsevnqrvfsldevfeianfnnylnqsgitkfntiiggkfvngentkrkg
ineyinlysqqindktlkkykmsvlfkqilsdtesksfvidkleddsdvvttmqsfyeqi
aafktveeksiketlsllfddlkaqkldlskiyfkndksltdlsqqvfddysvigtavle
yitqqiapknldnpskkeqeliakktekakylsletiklaleefnkhrdidkqcrfeeil
anfaaipmifdeiaqnkdnlaqisikyqnqgkkdllqasaeddvkaikdlldqtnnllhk
lkifhisqsedkanildkdehfylvfeecyfelanivplynkirnyitqkpysdekfkln
fenstlangwdknkepdntailfikddkyylgvmnkknnkifddkaikenkgegykkivy
kllpgankmlpkvffsaksikfynpsedilrirnhsthtkngspqkgyekfefniedcrk
fidfykqsiskhpewkdfgfrfsdtqrynsidefyrevenqgykltfenisesyidsvvn
qgklylfqiynkdfsayskgrpnlhtlywkalfdernlqdvvyklngeaelfyrkqsipk
kithpakeaianknkdnpkkesvfeydlikdkrftedkfffhcpitinfkssgankfnde
inlllkekandvhilsidrgerhlayytlvdgkgniikqdtfniigndrmktnyhdklaa
iekdrdsarkdwkkinnikemkegylsqvvheiaklvieynaivvfqdlnfgfkrgrfkv
ekqvyqklekmlieklnylvfkdnefdktggvlrayqltapfetfkkmgkqtgiiyyvpa
gftskicpvtgfvnqlypkyesvsksqeffskfdkicynldkgyfefsfdyknfgdkaak
gkwtiasfgsrlinfrnsdknhnwdtrevyptkelekllkdysieyghgecikaaicges
dkkffakltsvlntilqmrnsktgteldylispvadvngnffdsrqapknmpqdadanga
yhiglkglmllgriknnqegkklnlvikneeyfefvqnrnn

    Sequence, based on observed residues (ATOM records):
    >6i1lA (A:)
nasiyqefvnkyslsktlrfelipqgktlenikarglilddekrakdykkakqiidkyhq
ffieeilssvcisedllqnysdvyfklkksdddnlqkdfksakdtikkqiseyikdsekf
knlfnqnlidakkgqesdlilwlkqskdngielfkansditdidealeiiksfkgwttyf
kgfhenrknvyssndiptsiiyrivddnlpkflenkakyeslkdkapeainyeqikkdla
eeltfdidyktsevnqrvfsldevfeianfnnylnqsgitkfntiiggkfvngentkrkg
ineyinlysqqindktlkkykmsvlfkqilsdtesksfvidkleddsdvvttmqsfyeqi
aafktveeksiketlsllfddlkaqkldlskiyfkndksltdlsqqvfddysvigtavle
yitqqiapknldnpskkeqeliakktekakylsletiklaleefnkhrdidkqcrfeeil
anfaaipmifdeiaqnkdnlaqisikyqnqgkkdllqasaeddvkaikdlldqtnnllhk
lkifhisqseildkdehfylvfeecyfelanivplynkirnyitqkpysdekfklnfens
tlangwdknkepdntailfikddkyylgvmnkknnkifddkaikenkgegykkivykllp
gankmlpkvffsaksikfynpsedilrirnhsthtkngspqkgyekfefniedcrkfidf
ykqsiskhpewkdfgfrfsdtqrynsidefyrevenqgykltfenisesyidsvvnqgkl
ylfqiynkdfsayskgrpnlhtlywkalfdernlqdvvyklngeaelfyrkqsipkkith
pakeaianknkkesvfeydlikdkrftedkfffhcpitinfkssgankfndeinlllkek
andvhilsidrgerhlayytlvdgkgniikqdtfniigndrmktnyhdklaaiekdrdsa
rkdwkkinnikemkegylsqvvheiaklvieynaivvfqdlrgrfkvekqvyqklekmli
eklnylvfkdnefdktggvlrayqltapfetfkkmgkqtgiiyyvpagftskicpvtgfv
nqlypkyesvsksqeffskfdkicynldkgyfefsfdyknfgdkaakgkwtiasfgsrli
nfrwdtrevyptkelekllkdysieyghgecikaaicgesdkkffakltsvlntilqmrn
sktgteldylispvadvngnffdsrqapknmpqdadangayhiglkglmllgriknnkkl
nlvikneeyfefvqnrn

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6i1lD (D:)
nasiyqefvnkyslsktlrfelipqgktlenikarglilddekrakdykkakqiidkyhq
ffieeilssvcisedllqnysdvyfklkksdddnlqkdfksakdtikkqiseyikdsekf
knlfnqnlidakkgqesdlilwlkqskdngielfkansditdidealeiiksfkgwttyf
kgfhenrknvyssndiptsiiyrivddnlpkflenkakyeslkdkapeainyeqikkdla
eeltfdidyktsevnqrvfsldevfeianfnnylnqsgitkfntiiggkfvngentkrkg
ineyinlysqqindktlkkykmsvlfkqilsdtesksfvidkleddsdvvttmqsfyeqi
aafktveeksiketlsllfddlkaqkldlskiyfkndksltdlsqqvfddysvigtavle
yitqqiapknldnpskkeqeliakktekakylsletiklaleefnkhrdidkqcrfeeil
anfaaipmifdeiaqnkdnlaqisikyqnqgkkdllqasaeddvkaikdlldqtnnllhk
lkifhisqsedkanildkdehfylvfeecyfelanivplynkirnyitqkpysdekfkln
fenstlangwdknkepdntailfikddkyylgvmnkknnkifddkaikenkgegykkivy
kllpgankmlpkvffsaksikfynpsedilrirnhsthtkngspqkgyekfefniedcrk
fidfykqsiskhpewkdfgfrfsdtqrynsidefyrevenqgykltfenisesyidsvvn
qgklylfqiynkdfsayskgrpnlhtlywkalfdernlqdvvyklngeaelfyrkqsipk
kithpakeaianknkdnpkkesvfeydlikdkrftedkfffhcpitinfkssgankfnde
inlllkekandvhilsidrgerhlayytlvdgkgniikqdtfniigndrmktnyhdklaa
iekdrdsarkdwkkinnikemkegylsqvvheiaklvieynaivvfqdlnfgfkrgrfkv
ekqvyqklekmlieklnylvfkdnefdktggvlrayqltapfetfkkmgkqtgiiyyvpa
gftskicpvtgfvnqlypkyesvsksqeffskfdkicynldkgyfefsfdyknfgdkaak
gkwtiasfgsrlinfrnsdknhnwdtrevyptkelekllkdysieyghgecikaaicges
dkkffakltsvlntilqmrnsktgteldylispvadvngnffdsrqapknmpqdadanga
yhiglkglmllgriknnqegkklnlvikneeyfefvqnrnn

    Sequence, based on observed residues (ATOM records):
    >6i1lD (D:)
asiyqefvnkyslsktlrfelipqgktlenikarglilddekrakdykkakqiidkyhqf
fieeilssvcisedllqnysdvyfklkksdddnlqkdfksakdtikkqiseyikdsekfk
nlfnqnlidakkgqesdlilwlkqskdngielfkansditdidealeiiksfkgwttyfk
gfhenrknvyssndiptsiiyrivddnlpkflenkakyeslkdkapeainyeqikkdlae
eltfdidyktsevnqrvfsldevfeianfnnylnqsgitkfntiiggkfvngentkrkgi
neyinlysqqindktlkkykmsvlfkqilsdtesksfvidkleddsdvvttmqsfyeqia
afktveeksiketlsllfddlkaqkldlskiyfkndksltdlsqqvfddysvigtavley
itqqiapiakktekakylsletiklaleefnkhrdidkqcrfeeilanfaaipmifdeia
qnkdnlaqisikyqnqgkkdllqasaeddvkaikdlldqtnnllhklkifhisqsenild
kdehfylvfeecyfelanivplynkirnyitqkpysdekfklnfenstlangwdknkepd
ntailfikddkyylgvmnkknnkifddkaikenkgegykkivykllpgankmlpkvffsa
ksikfynpsedilrirnhsthtkngspqkgyekfefniedcrkfidfykqsiskhpewkd
fgfrfsdtqrynsidefyrevenqgykltfenisesyidsvvnqgklylfqiynkdfsay
skgrpnlhtlywkalfdernlqdvvyklngeaelfyrkqsipkkithpakeaianknkdn
pkkesvfeydlikdkrftedkfffhcpitinfkssgankfndeinlllkekandvhilsi
drgerhlayytlvdgkgniikqdtfniigmktnyhdklaaiekdrdsarkdwkkinnike
mkegylsqvvheiaklvieynaivvfqdlfkvekqvyqklekmlieklnylvfkdnefdk
tggvlrayqltapfetfkkmgkqtgiiyyvpagftskicpvtgfvnqlypkyesvsksqe
ffskfdkicynldkgyfefsfdyknfgdkaakgkwtiasfgsrlitrevyptkelekllk
dysieyghgecikaaicgesdkkffakltsvlntilqmrnsktdylispvadvngnffds
rqapknmpqdadangayhiglkglmllgriknnqklnlvikneeyfefvqnrnn

  • Chain 'E':
    No sequence available.

  • Chain 'F':
    No sequence available.