PDB entry 6i00

View 6i00 on RCSB PDB site
Description: cryo-em informed directed evolution of nitrilase 4 leads to a change in quaternary structure.
Deposited on 2018-10-24, released 2019-07-24
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 3.4 Å
R-factor: N/A
AEROSPACI score: 0.09 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'I':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'K':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):
  • Chain 'L':
    Compound: Bifunctional nitrilase/nitrile hydratase NIT4
    Species: Arabidopsis thaliana [TaxId:3702]
    Gene: NIT4, At5g22300, MWD9.8
    Database cross-references and differences (RAF-indexed):

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6i00A (A:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00A (A:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6i00B (B:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00B (B:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6i00C (C:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00C (C:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6i00D (D:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00D (D:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6i00E (E:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00E (E:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6i00F (F:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00F (F:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'G':
    Sequence, based on SEQRES records:
    >6i00G (G:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00G (G:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'H':
    Sequence, based on SEQRES records:
    >6i00H (H:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00H (H:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'I':
    Sequence, based on SEQRES records:
    >6i00I (I:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00I (I:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'J':
    Sequence, based on SEQRES records:
    >6i00J (J:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00J (J:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'K':
    Sequence, based on SEQRES records:
    >6i00K (K:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00K (K:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts

  • Chain 'L':
    Sequence, based on SEQRES records:
    >6i00L (L:)
msmqqetshmtaapqtnghqifpeidmsagdsssivratvvqastvfydtpatldkaerl
lseaaengsqlvvfpeafiggyprgstfelaigsrtakgrddfrkyhasaidvpgpever
lalmakkykvylvmgvieregytlyctvlffdsqglflgkhrklmptalerciwgfgdgs
tipvfdtpigkigaaicwenrmpslrtamyakgieiycaptadsretwlasmthialegg
cfvlsanqfcrrkdypsppeymfsgseesltpdsvvcaggssiisplgivlagpnyrgea
litadldlgdiarakfdfdvvghysrpevfslnirehprkavsfktskvmedesvhhhhh
h

    Sequence, based on observed residues (ATOM records):
    >6i00L (L:)
ivratvvqastvfydtpatldkaerllseaaengsqlvvfpeafiggyprgstfelaigs
rtakgrddfrkyhasaidvpgpeverlalmakkykvylvmgvieregytlyctvlffdsq
glflgkhrklmptalerciwgfgdgstipvfdtpigkigaaicwenrmpslrtamyakgi
eiycaptadsretwlasmthialeggcfvlsanqfcrvcaggssiisplgivlagpnyrg
ealitadldlgdiarakfdfdvvghysrpevfslnirehprkavsfkts