PDB entry 6gtd

View 6gtd on RCSB PDB site
Description: transient state structure of crispr-cpf1 (cas12a) i2 conformation
Deposited on 2018-06-18, released 2018-12-19
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 4.24 Å
R-factor: N/A
AEROSPACI score: -0.02 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: CRISPR-associated endonuclease Cas12a
    Species: Francisella tularensis subsp. novicida (strain U112) [TaxId:401614]
    Gene: cas12a, cpf1, FTN_1397
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0Q7Q2 (0-End)
      • conflict (1005)
  • Chain 'B':
    Compound: RNA (28-mer)
    Species: Francisella tularensis subsp. novicida U112, synthetic [TaxId:401614]
  • Chain 'C':
    Compound: DNA (5'-d(p*tp*gp*ap*cp*tp*tp*cp*tp*cp*tp*ap*ap*cp*ap*ap*gp*cp*tp*cp*g)-3')
    Species: Francisella tularensis subsp. novicida U112, synthetic [TaxId:401614]
  • Chain 'D':
    Compound: DNA (5'-d(p*cp*gp*ap*gp*cp*tp*cp*gp*tp*tp*ap*gp*ap*gp*ap*ap*gp*t)-3')
    Species: Francisella tularensis subsp. novicida U112, synthetic [TaxId:401614]

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6gtdA (A:)
    msiyqefvnkyslsktlrfelipqgktlenikarglilddekrakdykkakqiidkyhqf
    fieeilssvcisedllqnysdvyfklkksdddnlqkdfksakdtikkqiseyikdsekfk
    nlfnqnlidakkgqesdlilwlkqskdngielfkansditdidealeiiksfkgwttyfk
    gfhenrknvyssndiptsiiyrivddnlpkflenkakyeslkdkapeainyeqikkdlae
    eltfdidyktsevnqrvfsldevfeianfnnylnqsgitkfntiiggkfvngentkrkgi
    neyinlysqqindktlkkykmsvlfkqilsdtesksfvidkleddsdvvttmqsfyeqia
    afktveeksiketlsllfddlkaqkldlskiyfkndksltdlsqqvfddysvigtavley
    itqqiapknldnpskkeqeliakktekakylsletiklaleefnkhrdidkqcrfeeila
    nfaaipmifdeiaqnkdnlaqisikyqnqgkkdllqasaeddvkaikdlldqtnnllhkl
    kifhisqsedkanildkdehfylvfeecyfelanivplynkirnyitqkpysdekfklnf
    enstlangwdknkepdntailfikddkyylgvmnkknnkifddkaikenkgegykkivyk
    llpgankmlpkvffsaksikfynpsedilrirnhsthtkngspqkgyekfefniedcrkf
    idfykqsiskhpewkdfgfrfsdtqrynsidefyrevenqgykltfenisesyidsvvnq
    gklylfqiynkdfsayskgrpnlhtlywkalfdernlqdvvyklngeaelfyrkqsipkk
    ithpakeaianknkdnpkkesvfeydlikdkrftedkfffhcpitinfkssgankfndei
    nlllkekandvhilsidrgerhlayytlvdgkgniikqdtfniigndrmktnyhdklaai
    ekdrdsarkdwkkinnikemkegylsqvvheiaklvieynaivvfqdlnfgfkrgrfkve
    kqvyqklekmlieklnylvfkdnefdktggvlrayqltapfetfkkmgkqtgiiyyvpag
    ftskicpvtgfvnqlypkyesvsksqeffskfdkicynldkgyfefsfdyknfgdkaakg
    kwtiasfgsrlinfrnsdknhnwdtrevyptkelekllkdysieyghgecikaaicgesd
    kkffakltsvlntilqmrnsktgteldylispvadvngnffdsrqapknmpqdadangay
    higlkglmllgriknnqegkklnlvikneeyfefvqnrnngsefelenlyfqgelrrqas
    alehhhhhh
    

    Sequence, based on observed residues (ATOM records):
    >6gtdA (A:)
    msiyqefvnkyslsktlrfelipqgktlenikarglilddekrakdykkakqiidkyhqf
    fieeilssvcisedllqnysdvyfklkksdddnlqkdfksakdtikkqiseyikdsekfk
    nlfnqnlidakkgqesdlilwlkqskdngielfkansditdidealeiiksfkgwttyfk
    gfhenrknvyssndiptsiiyrivddnlpkflenkakyeslkdkapeainyeqikkdlae
    eltfdidyktsevnqrvfsldevfeianfnnylnqsgitkfntiiggkfvngentkrkgi
    neyinlysqqindktlkkykmsvlfkqilsdtdkleddsdvvttmqsfyeqiaafktvee
    ksiketlsllfddlkaqkldlskiyfkndksltdlsqqvfddysvigtavleyitqqiap
    knqeliakktekakylsletiklaleefnkhrdidkqcrfeeilanfaaipmifdeiaqn
    kdnlaqisikyqnqgkkdllqasaeddvkaikdlldqtnnllhklkifhisqsedkanil
    dkdehfylvfeecyfelanivplynkirnyitqkpysdekfklnfenstlangwdknkep
    dntailfikddkyylgvmnkknnkifddkaikenkgegykkivykllpgankmlpkvffs
    aksikfynpsedilrirnhsthtkngspqkgyekfefniedcrkfidfykqsiskhpewk
    dfgfrfsdtqrynsidefyrevenqgykltfenisesyidsvvnqgklylfqiynkdfsa
    yskgrpnlhtlywkalfdernlqdvvyklngeaelfyrkqsipkkithpakeaianknkd
    npkkesvfeydlikdkrftedkfffhcpitinfkssgankfndeinlllkekandvhils
    idrgerhlayytlvdgkgniikqdtfniigndrmktnyhdklaaiekdrdsarkdwkkin
    nikemkegylsqvvheiaklvieynaivvfqdlnkqvyqklekmlieklnylvfkdnefd
    ktggvlrayqltapfetfkkmgkqtgiiyyvpagftskicpvtgfvnqlypkyesvsksq
    effskfdkicynldkgyfefsfdyknfgdkaakgkwtiasfgsrlinfrnsnwdtrevyp
    tkelekllkdysieyghgecikaaicgesdkkffakltsvlntilqmrnsktgteldyli
    spvadvngnffdsrqapknmpqdadangayhiglkglmllgriknnqegkklnlviknee
    yfefvqnrn
    

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.

  • Chain 'D':
    No sequence available.