PDB entry 6fbu

View 6fbu on RCSB PDB site
Description: crystal structure of the dna repair enzyme endonuclease-viii (nei) from e. coli (e2q) in complex with ap-site containing dna substrate
Deposited on 2017-12-19, released 2019-01-30
The last revision was dated 2019-01-30, with a file datestamp of 2019-01-25.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Endonuclease 8
    Species: Escherichia coli [TaxId:83333]
    Gene: nei, b0714, JW0704
    Database cross-references and differences (RAF-indexed):
    • Uniprot P50465 (0-261)
      • engineered mutation (1)
      • engineered mutation (33)
      • engineered mutation (111)
  • Chain 'B':
    Compound: DNA (5'-d(p*gp*gp*cp*tp*tp*cp*ap*tp*cp*cp*tp*g)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Chain 'C':
    Compound: DNA (5'-d(p*cp*cp*ap*gp*gp*ap*(ped)p*gp*ap*ap*gp*cp*c)-3')
    Species: synthetic construct, synthetic [TaxId:32630]
  • Heterogens: SO4, ZN, ACT, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6fbuA (A:)
    pqgpeirraadnleaaikgkpltdvwfafpqlktyqsqligqhvthvetrgkallthfsn
    dltlyshnqlygvwrvvdtgeepqttrvlrvklqtadktillysasdiemlrpeqltthp
    flqrvgpdvldpnltpevvkerllsprfrnrqfagllldqaflaglgnylrveilwqvgl
    tgnhkakdlnaaqldalahalleiprfsyatrgqvdenkhhgalfrfkvfhrdgepcerc
    gsiiekttlssrpfywcpgcqh
    

    Sequence, based on observed residues (ATOM records):
    >6fbuA (A:)
    pqgpeirraadnleaaikgkpltdvwfafpqlktyqsqligqhvthvetrgkallthfsn
    dltlyshnqlygvwrvvdtgeepqttrvlrvklqtadktillysasdiemlrpeqltthp
    flqrvgpdvldpnltpevvkerllsprfrnrqfagllldqaflaglgnylrveilwqvgl
    tgnhkakdlnaaqldalahalleiprfsyatrgqalfrfkvfhrdgepcercgsiiektt
    lssrpfywcpgcqh
    

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.