PDB entry 6efu

View 6efu on RCSB PDB site
Description: Crystal structure of the double mutant L167W / P172L of the beta-glucosidase from Trichoderma harzianum
Class: hydrolase
Keywords: gh1, hydrolase
Deposited on 2018-08-17, released 2019-06-26
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-01-01, with a file datestamp of 2019-12-27.
Experiment type: XRAY
Resolution: 2.2 Å
R-factor: N/A
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Beta-glucosidase
    Species: TRICHODERMA HARZIANUM [TaxId:5544]
    Gene: bgl, CI102_8745
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A127SA86 (1-End)
      • expression tag (0)
      • engineered mutation (167)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6efua1, d6efua2
  • Chain 'B':
    Compound: Beta-glucosidase
    Species: TRICHODERMA HARZIANUM [TaxId:5544]
    Gene: bgl, CI102_8745
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A127SA86 (1-End)
      • engineered mutation (167)
      • engineered mutation (172)
    Domains in SCOPe 2.08: d6efub_
  • Heterogens: NO3, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >6efuA (A:)
hmlpkdfqwgfataayqiegaidkdgrgpsiwdtfcaipgkiadgtsgvtacdsynrtae
diallkslgaksyrfsiswsriipkggrddpvnqlgidhyaqfvddlleagitpfitlfh
wdlpeelhqryggllnrtefpldfenyarvmfkalpkvrnwitfnepwcsailgygsgtf
apgrqsttepwivghnllvahgravkvyrdefkdlndgqigivlngdftypwdssdpldr
eaaerrlefftawyadpiylgdypasmrkqlgdrlpeftpeekafvlgsndfygmnhyts
nyirhrtspataddtvgnvdvlfynkegqcigpetesswlrpcpagfrdflvwiskryny
pkiyvtengtslkgendlpkekileddfrvnyyneyiramftaatldgvnvkgyfawslm
dnfewadgyvtrfgvtyvdyengqqrfpkksakslkplfdeliake

    Sequence, based on observed residues (ATOM records): (download) 
    >6efuA (A:)
hmlpkdfqwgfataayqiegaidkdgrgpsiwdtfcaipgkiadgtsgvtacdsynrtae
diallkslgaksyrfsiswsriipkggrddpvnqlgidhyaqfvddlleagitpfitlfh
wdlpeelhqryggllnrtefpldfenyarvmfkalpkvrnwitfnepwcsailgygsgtf
apgrqsttepwivghnllvahgravkvyrdefkdlndgqigivlngdftypwdssdpldr
eaaerrlefftawyadpiylgdypasmrkqlgdrlpeftpeekafvlgsndfygmnhyts
nyirhrtspataddtvgnvdvlfynkegqcigpetesswlrpcpagfrdflvwiskryny
pkiyvtengtslkgendlpkekileddfrvnyyneyiramftaatldgvnvkgyfawslm
dnfewadgyvtrfgvtyvdyengqqrfpkksakslkplfdeliak

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >6efuB (B:)
hmlpkdfqwgfataayqiegaidkdgrgpsiwdtfcaipgkiadgtsgvtacdsynrtae
diallkslgaksyrfsiswsriipkggrddpvnqlgidhyaqfvddlleagitpfitlfh
wdlpeelhqryggllnrtefpldfenyarvmfkalpkvrnwitfnepwcsailgygsgtf
apgrqsttepwivghnllvahgravkvyrdefkdlndgqigivlngdftypwdssdpldr
eaaerrlefftawyadpiylgdypasmrkqlgdrlpeftpeekafvlgsndfygmnhyts
nyirhrtspataddtvgnvdvlfynkegqcigpetesswlrpcpagfrdflvwiskryny
pkiyvtengtslkgendlpkekileddfrvnyyneyiramftaatldgvnvkgyfawslm
dnfewadgyvtrfgvtyvdyengqqrfpkksakslkplfdeliake

    Sequence, based on observed residues (ATOM records): (download) 
    >6efuB (B:)
mlpkdfqwgfataayqiegaidkdgrgpsiwdtfcaipgkiadgtsgvtacdsynrtaed
iallkslgaksyrfsiswsriipkggrddpvnqlgidhyaqfvddlleagitpfitlfhw
dlpeelhqryggllnrtefpldfenyarvmfkalpkvrnwitfnepwcsailgygsgtfa
pgrqsttepwivghnllvahgravkvyrdefkdlndgqigivlngdftypwdssdpldre
aaerrlefftawyadpiylgdypasmrkqlgdrlpeftpeekafvlgsndfygmnhytsn
yirhrtspataddtvgnvdvlfynkegqcigpetesswlrpcpagfrdflvwiskrynyp
kiyvtengtslkgendlpkekileddfrvnyyneyiramftaatldgvnvkgyfawslmd
nfewadgyvtrfgvtyvdyengqqrfpkksakslkplfdeliak